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Magnesium in PDB 6dp4: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C, PDB code: 6dp4 was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.92 / 1.37
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.525, 37.674, 62.050, 90.00, 96.52, 90.00
R / Rfree (%) 15.7 / 17.6

Other elements in 6dp4:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C also contains other interesting chemical elements:

Potassium (K) 3 atoms
Iodine (I) 3 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C (pdb code 6dp4). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C, PDB code: 6dp4:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dp4

Go back to Magnesium Binding Sites List in 6dp4
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:21.4
occ:1.00
OD2 A:ASP71 2.0 21.9 1.0
OP3 b:C5 2.0 26.5 0.8
O A:HOH301 2.0 26.2 0.2
OD1 A:ASP192 2.1 24.0 1.0
O A:HOH311 2.1 23.1 1.0
O A:HOH310 2.1 24.6 1.0
OP1 b:C5 2.3 30.7 0.2
OP1 b:C5 2.4 26.4 0.8
P b:C5 2.8 24.1 0.8
CG A:ASP71 3.0 17.6 1.0
HA A:ASP192 3.2 27.7 1.0
CG A:ASP192 3.2 24.0 1.0
P b:C5 3.4 25.1 0.2
OD1 A:ASP71 3.4 18.5 1.0
HB2 A:ASP192 3.6 28.3 1.0
H A:TYR193 3.6 29.6 1.0
OP2 b:C5 3.7 30.0 0.2
CB A:ASP192 3.7 23.6 1.0
MG A:MG202 3.8 20.6 1.0
NZ A:LYS196 3.8 31.8 0.8
CA A:ASP192 3.9 23.1 1.0
OP2 b:C5 3.9 26.3 0.8
O5' b:C5 3.9 20.7 0.8
K A:K203 3.9 27.2 0.1
OP2 b:G6 4.0 27.1 1.0
OE2 A:GLU188 4.0 50.6 1.0
OD2 A:ASP132 4.0 32.2 1.0
O A:VAL72 4.0 20.6 1.0
O b:HOH101 4.1 29.2 0.9
HG22 A:ILE189 4.1 26.3 1.0
O5' b:C5 4.2 28.1 0.2
C5' b:C5 4.2 23.4 1.0
OD2 A:ASP192 4.2 25.6 1.0
N A:TYR193 4.3 24.7 1.0
CB A:ASP71 4.4 18.7 1.0
HB3 A:ASP71 4.5 22.5 1.0
K A:K204 4.5 28.5 0.4
HD1 A:TYR193 4.5 25.9 1.0
O3' B:U4 4.7 21.0 0.2
HB3 A:ASP192 4.7 28.3 1.0
HG21 A:ILE189 4.7 26.3 1.0
HB2 A:ASP71 4.7 22.5 1.0
C A:ASP192 4.7 24.9 1.0
CG2 A:ILE189 4.8 21.9 1.0
HB2 A:TYR193 4.9 34.3 1.0
N A:ASP192 5.0 22.6 1.0
HG23 A:ILE189 5.0 26.3 1.0
CE A:LYS196 5.0 36.3 0.8

Magnesium binding site 2 out of 2 in 6dp4

Go back to Magnesium Binding Sites List in 6dp4
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 20 Mm MG2+ and 75 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg202

b:20.6
occ:1.00
OD1 A:ASP71 2.0 18.5 1.0
OE1 A:GLU109 2.1 21.3 1.0
OP1 b:C5 2.1 26.4 0.8
OP1 b:C5 2.1 30.7 0.2
OD1 A:ASP132 2.2 22.8 1.0
OD2 A:ASP132 2.4 32.2 1.0
CG A:ASP132 2.6 25.6 1.0
O3' B:U4 2.6 21.0 0.2
P b:C5 2.9 25.1 0.2
CD A:GLU109 3.0 18.6 1.0
CG A:ASP71 3.0 17.6 1.0
O A:HOH349 3.1 18.9 1.0
OE2 A:GLU109 3.2 19.0 1.0
P b:C5 3.4 24.1 0.8
OD2 A:ASP71 3.5 21.9 1.0
HG12 A:VAL72 3.5 23.2 1.0
C3' B:U4 3.6 23.1 0.8
O3' B:U4 3.7 25.5 0.8
H A:VAL72 3.7 22.5 1.0
MG A:MG201 3.8 21.4 1.0
O A:HOH311 3.8 23.1 1.0
HA A:ASP71 3.9 18.9 1.0
O5' b:C5 4.0 28.1 0.2
C3' B:U4 4.0 28.2 0.2
OP2 b:C5 4.0 30.0 0.2
OP2 b:C5 4.1 26.3 0.8
CB A:ASP132 4.1 20.8 1.0
H A:ASP132 4.1 22.1 1.0
C5' b:C5 4.2 23.4 1.0
CB A:ASP71 4.2 18.7 1.0
O5' b:C5 4.2 20.7 0.8
HB2 A:SER133 4.3 23.0 1.0
OP3 b:C5 4.3 26.5 0.8
HG21 A:ILE189 4.3 26.3 1.0
CG A:GLU109 4.4 17.4 1.0
C4' B:U4 4.5 26.7 0.2
N A:VAL72 4.5 18.7 1.0
HB2 A:ASP71 4.5 22.5 1.0
CG1 A:VAL72 4.5 19.4 1.0
HB3 A:ASP132 4.5 24.9 1.0
CA A:ASP71 4.5 15.8 1.0
O2' B:U4 4.5 18.5 0.2
HB2 A:ASP132 4.5 24.9 1.0
C4' B:U4 4.6 27.7 0.8
HG3 A:GLU109 4.6 20.8 1.0
C5' B:U4 4.7 31.2 0.2
HG2 A:GLU109 4.7 20.8 1.0
C5' B:U4 4.8 27.1 0.8
C2' B:U4 4.8 21.2 0.8
N A:ASP132 4.8 18.4 1.0
CA A:ASP132 4.8 19.8 1.0
O A:HOH310 4.8 24.6 1.0
HG13 A:VAL72 4.8 23.2 1.0
HG22 A:ILE189 4.8 26.3 1.0
C2' B:U4 4.8 25.6 0.2
HG11 A:VAL72 4.9 23.2 1.0
O A:HOH301 4.9 26.2 0.2
O A:VAL72 4.9 20.6 1.0
C A:ASP132 4.9 18.1 1.0
HB3 A:ASP71 5.0 22.5 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Sep 30 23:24:26 2024

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