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Magnesium in PDB 6gal: Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10

Enzymatic activity of Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10

All present enzymatic activity of Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10:
1.12.99.6;

Protein crystallography data

The structure of Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10, PDB code: 6gal was solved by S.B.Carr, F.A.Armstrong, R.M.Evans, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 92.12 / 1.25
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 94.906, 95.224, 183.910, 90.00, 90.00, 90.00
R / Rfree (%) 12 / 14.7

Other elements in 6gal:

The structure of Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10 also contains other interesting chemical elements:

Nickel (Ni) 2 atoms
Iron (Fe) 24 atoms
Chlorine (Cl) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10 (pdb code 6gal). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10, PDB code: 6gal:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6gal

Go back to Magnesium Binding Sites List in 6gal
Magnesium binding site 1 out of 2 in the Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10 within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Mg602

b:4.1
occ:1.00
HE2 L:HIS582 1.3 5.2 0.0
O L:HOH725 2.1 6.4 1.0
O L:HOH711 2.1 6.6 1.0
O L:CYS528 2.1 5.9 1.0
O L:HOH726 2.1 5.8 1.0
OE1 L:GLU57 2.2 6.1 1.0
NE2 L:HIS582 2.2 5.4 1.0
CD L:GLU57 3.1 5.8 1.0
H L:CYS528 3.1 5.0 1.0
CE1 L:HIS582 3.1 5.5 1.0
CD2 L:HIS582 3.2 6.2 1.0
HE1 L:HIS582 3.3 5.3 1.0
C L:CYS528 3.3 5.1 1.0
HZ1 L:LYS399 3.3 6.6 1.0
HD2 L:HIS582 3.4 5.8 1.0
OE2 L:GLU57 3.4 6.7 1.0
HB2 L:CYS528 3.7 6.3 1.0
HD2 L:LYS399 3.7 6.9 1.0
N L:CYS528 3.8 5.2 1.0
HA L:VAL529 4.0 5.7 1.0
CA L:CYS528 4.0 5.7 1.0
OE2 L:GLU347 4.1 7.0 1.0
OE1 L:GLU347 4.2 7.9 1.0
HG21 L:VAL529 4.2 7.0 1.0
OE1 L:GLN527 4.2 8.3 1.0
O L:HOH1014 4.2 6.1 1.0
NZ L:LYS399 4.3 6.5 1.0
ND1 L:HIS582 4.3 5.9 1.0
O L:HOH828 4.3 6.5 1.0
CB L:CYS528 4.3 6.3 1.0
CG L:HIS582 4.3 5.7 1.0
N L:VAL529 4.4 5.5 1.0
CG L:GLU57 4.4 6.7 1.0
HE3 L:LYS399 4.5 6.4 1.0
CD L:LYS399 4.5 7.1 1.0
CD L:GLU347 4.6 7.2 1.0
HG2 L:GLU57 4.6 6.5 1.0
HG3 L:GLU57 4.6 7.0 1.0
HG1 L:THR370 4.6 7.0 0.0
CA L:VAL529 4.7 5.8 1.0
HA L:GLN527 4.7 5.7 1.0
CE L:LYS399 4.7 6.4 1.0
HZ3 L:LYS399 4.7 6.6 1.0
HZ2 L:LYS399 4.8 6.5 1.0
HB3 L:GLN527 4.8 5.8 1.0
HG22 L:THR533 4.8 5.8 1.0
HG23 L:VAL530 4.9 7.1 1.0
HG3 L:LYS399 4.9 7.4 1.0
HB3 L:CYS528 4.9 6.3 1.0
HG1 L:THR533 4.9 6.1 0.0
C L:GLN527 4.9 5.4 1.0
HA L:CYS528 4.9 5.6 1.0

Magnesium binding site 2 out of 2 in 6gal

Go back to Magnesium Binding Sites List in 6gal
Magnesium binding site 2 out of 2 in the Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Fully Reduced Hydrogenase (Hyd-1) Variant E28Q Collected at pH 10 within 5.0Å range:
probe atom residue distance (Å) B Occ
M:Mg603

b:5.4
occ:1.00
HE2 M:HIS582 1.3 6.3 0.0
O M:HOH719 2.1 7.5 1.0
O M:HOH716 2.1 8.1 1.0
O M:CYS528 2.1 7.7 1.0
O M:HOH720 2.1 7.9 1.0
OE1 M:GLU57 2.2 6.8 1.0
NE2 M:HIS582 2.2 6.5 1.0
CE1 M:HIS582 3.1 6.3 1.0
CD M:GLU57 3.1 7.2 1.0
H M:CYS528 3.1 6.8 1.0
HE1 M:HIS582 3.2 6.3 1.0
CD2 M:HIS582 3.2 7.5 1.0
C M:CYS528 3.3 6.6 1.0
HZ1 M:LYS399 3.3 8.2 1.0
OE2 M:GLU57 3.4 7.5 1.0
HD2 M:HIS582 3.4 7.0 1.0
HD2 M:LYS399 3.6 8.0 1.0
HB2 M:CYS528 3.7 7.4 1.0
N M:CYS528 3.8 6.8 1.0
CA M:CYS528 4.0 6.5 1.0
HA M:VAL529 4.0 7.5 1.0
OE2 M:GLU347 4.1 9.0 1.0
OE1 M:GLU347 4.2 8.7 1.0
OE1 M:GLN527 4.2 9.5 1.0
HG21 M:VAL529 4.2 9.3 1.0
ND1 M:HIS582 4.3 6.5 1.0
O M:HOH969 4.3 7.6 1.0
NZ M:LYS399 4.3 8.1 1.0
O M:HOH782 4.3 7.6 1.0
CB M:CYS528 4.3 7.4 1.0
CG M:HIS582 4.3 6.6 1.0
N M:VAL529 4.4 6.9 1.0
CG M:GLU57 4.4 7.0 1.0
HE3 M:LYS399 4.5 7.7 1.0
CD M:LYS399 4.5 7.9 1.0
CD M:GLU347 4.5 8.8 1.0
HG2 M:GLU57 4.6 7.2 1.0
HG3 M:GLU57 4.6 6.9 1.0
HG1 M:THR370 4.6 8.1 0.0
HA M:GLN527 4.7 7.9 1.0
CA M:VAL529 4.7 7.4 1.0
CE M:LYS399 4.7 7.9 1.0
HZ3 M:LYS399 4.8 8.1 1.0
HZ2 M:LYS399 4.8 8.1 1.0
HB3 M:GLN527 4.8 8.3 1.0
HG22 M:THR533 4.8 6.5 1.0
HB3 M:CYS528 4.8 7.4 1.0
HG3 M:LYS399 4.9 8.4 1.0
HG23 M:VAL530 4.9 8.0 1.0
C M:GLN527 4.9 7.0 1.0
HA M:CYS528 4.9 6.7 1.0
HG1 M:THR533 4.9 6.6 0.0

Reference:

R.M.Evans, P.A.Ash, S.E.Beaton, E.J.Brooke, K.A.Vincent, S.B.Carr, F.A.Armstrong. Mechanistic Exploitation of A Self-Repairing, Blocked Proton Transfer Pathway in An O2-Tolerant [Nife]-Hydrogenase. J. Am. Chem. Soc. V. 140 10208 2018.
ISSN: ESSN 1520-5126
PubMed: 30070475
DOI: 10.1021/JACS.8B04798
Page generated: Tue Oct 1 01:03:23 2024

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