Magnesium in PDB 6xwd: 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif

The structure of 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif, PDB code: 6xwd was solved by S.Kiehstaller, S.Hennig, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.37 / 1.60
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	82.000, 111.600, 62.400, 90.00, 90.00, 90.00
R / Rfree (%)	16.6 / 19.6

The structure of 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif also contains other interesting chemical elements:

Calcium	(Ca)	1 atom
Sodium	(Na)	2 atoms

The binding sites of Magnesium atom in the 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif (pdb code 6xwd). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif, PDB code: 6xwd:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg304 b:17.9 occ:1.00 O A:HOH442 2.3 31.1 1.0 O A:GLU110 2.3 21.9 0.4 O A:GLU110 2.4 22.3 0.6 O A:HOH648 2.4 30.8 1.0 OE1 A:GLU35 2.4 29.6 1.0 OE2 A:GLU35 2.9 34.9 1.0 CD A:GLU35 3.0 35.4 1.0 C A:GLU110 3.5 20.3 0.4 C A:GLU110 3.6 20.3 0.6 N A:GLY112 4.0 26.2 1.0 CA A:GLU110 4.4 18.1 0.4 CA A:GLU110 4.4 18.0 0.6 OE1 A:GLU110 4.4 26.6 0.6 CG A:GLU35 4.4 22.7 1.0 O A:HOH519 4.5 31.1 1.0 N A:ALA111 4.5 16.9 1.0 CB A:GLU110 4.5 20.0 0.4 O A:HOH666 4.6 35.1 1.0 CB A:GLU110 4.6 20.2 0.6 CA A:ALA111 4.6 17.4 1.0 C A:ALA111 4.7 21.4 1.0 CA A:GLY112 4.7 26.6 1.0

Magnesium binding site 2 out of 2 in the 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg305 b:17.0 occ:0.50 O A:HOH659 2.3 47.1 1.0 OE1 A:GLU2 2.4 22.7 1.0 O A:HOH469 2.5 20.2 1.0 O A:HOH692 2.8 39.7 1.0 CD A:GLU2 3.4 20.5 1.0 OE2 A:GLU2 3.8 20.7 1.0 O A:HOH622 4.3 20.8 1.0 O A:HOH773 4.5 23.1 1.0 CG A:GLU2 4.7 15.7 1.0 CA A:GLU2 4.9 16.9 1.0 O A:HOH446 4.9 29.6 1.0 N A:ARG3 5.0 14.3 1.0 CB A:GLU2 5.0 16.1 1.0

S.Kiehstaller, C.Ottmann, S.Hennig. Mmp Activation Associated Aminopeptidase N Reveals A Bivalent 14-3-3 Binding Motif. J.Biol.Chem. 2020.
ISSN: ESSN 1083-351X
PubMed: 33109610
DOI: 10.1074/JBC.RA120.014708