Magnesium in PDB 7bdj: Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Atpgammas

All present enzymatic activity of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Atpgammas:
3.6.4.13;

The structure of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Atpgammas, PDB code: 7bdj was solved by K.Vester, K.F.Santos, E.Absmeier, M.C.Wahl, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.34 / 2.59
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	99.612, 118.912, 187.353, 90, 90, 90
R / Rfree (%)	23 / 28.9

The binding sites of Magnesium atom in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Atpgammas (pdb code 7bdj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Atpgammas, PDB code: 7bdj:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Atpgammas


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Atpgammas within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2203 b:70.2 occ:1.00 O40 B:TGB2201 2.1 62.4 1.0 O39 B:TGB2201 2.3 69.5 1.0 OE1 B:GLU616 2.4 55.0 1.0 P38 B:TGB2201 2.6 84.8 1.0 OD1 B:ASP615 2.7 43.4 1.0 CD B:GLU616 3.3 54.0 1.0 CE B:LYS509 3.3 44.7 1.0 O37 B:TGB2201 3.6 83.9 1.0 NZ B:LYS509 3.7 41.0 1.0 CG B:ASP615 3.7 47.8 1.0 OE2 B:GLU616 4.0 55.1 1.0 CG B:GLU616 4.0 47.7 1.0 OD2 B:ASP615 4.1 50.1 1.0 CB B:LEU651 4.2 41.9 1.0 N B:GLU616 4.3 43.5 1.0 O35 B:TGB2201 4.3 76.5 1.0 S41 B:TGB2201 4.3 91.2 1.0 P34 B:TGB2201 4.3 80.5 1.0 O36 B:TGB2201 4.4 53.8 1.0 CB B:LYS509 4.5 46.4 1.0 CD B:LYS509 4.6 45.1 1.0 CD1 B:LEU651 4.6 45.7 1.0 CA B:GLU616 4.8 40.8 1.0 O B:LEU651 4.8 40.2 1.0 C B:LEU651 5.0 43.7 1.0 CG B:LYS509 5.0 49.1 1.0

Magnesium binding site 2 out of 2 in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Atpgammas


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Atpgammas within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2204 b:64.7 occ:1.00 O40 B:TGB2202 2.1 67.7 1.0 P38 B:TGB2202 2.8 88.5 1.0 CE B:LYS1356 2.8 48.3 1.0 OD1 B:ASP1454 2.9 52.9 1.0 O39 B:TGB2202 3.1 65.2 1.0 OE2 B:GLU1455 3.1 49.7 1.0 O37 B:TGB2202 3.2 59.9 1.0 NZ B:LYS1356 3.3 47.1 1.0 CD B:GLU1455 3.7 49.8 1.0 O35 B:TGB2202 3.7 47.9 1.0 CG B:ASP1454 3.8 45.9 1.0 P34 B:TGB2202 4.0 59.6 1.0 CB B:LYS1356 4.0 51.3 1.0 OD2 B:ASP1454 4.1 42.0 1.0 CD B:LYS1356 4.2 43.5 1.0 CG B:GLU1455 4.2 43.0 1.0 O36 B:TGB2202 4.2 72.3 1.0 N B:GLU1455 4.4 52.4 1.0 OE1 B:GLU1455 4.5 53.6 1.0 CB B:LEU1490 4.5 49.8 1.0 CG B:LYS1356 4.6 49.2 1.0 S41 B:TGB2202 4.7 72.0 1.0 CA B:GLU1455 4.9 46.3 1.0 N B:SER1491 4.9 46.9 1.0 C B:LEU1490 5.0 52.0 1.0

E.Absmeier, K.Vester, T.Ghane, D.Burakovskiy, P.Milon, P.Imhof, M.V.Rodnina, K.F.Santos, M.C.Wahl. Long-Range Allostery Mediates Cooperative Adenine Nucleotide Binding By the SKI2-Like Rna Helicase BRR2. J.Biol.Chem. 00829 2021.
ISSN: ESSN 1083-351X
PubMed: 34048711
DOI: 10.1016/J.JBC.2021.100829