Magnesium in PDB 7bg4: Multidrug Resistance Transporter Bmra Mutant E504A Bound with Atp, Mg, and Rhodamine 6G Solved By Cryo-Em

The binding sites of Magnesium atom in the Multidrug Resistance Transporter Bmra Mutant E504A Bound with Atp, Mg, and Rhodamine 6G Solved By Cryo-Em (pdb code 7bg4). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Multidrug Resistance Transporter Bmra Mutant E504A Bound with Atp, Mg, and Rhodamine 6G Solved By Cryo-Em, PDB code: 7bg4:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Multidrug Resistance Transporter Bmra Mutant E504A Bound with Atp, Mg, and Rhodamine 6G Solved By Cryo-Em


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Multidrug Resistance Transporter Bmra Mutant E504A Bound with Atp, Mg, and Rhodamine 6G Solved By Cryo-Em within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg603 b:44.6 occ:1.00 OG1 A:THR381 2.0 53.9 1.0 CB A:THR381 2.4 53.9 1.0 CG2 A:THR381 2.5 53.9 1.0 CA A:THR381 2.6 53.9 1.0 OE1 A:GLN422 2.8 53.5 1.0 CG A:GLN422 3.2 53.5 1.0 CD A:GLN422 3.2 53.5 1.0 OD2 A:ASP503 3.3 46.3 1.0 O1A A:ATP601 3.4 66.0 1.0 O1B A:ATP601 3.5 66.0 1.0 N A:THR381 3.6 53.9 1.0 C A:THR381 3.7 53.9 1.0 OD1 A:ASP503 3.8 46.3 1.0 O A:THR381 3.9 53.9 1.0 CG A:ASP503 4.0 46.3 1.0 NE2 A:GLN422 4.3 53.5 1.0 PB A:ATP601 4.4 66.0 1.0 C A:LYS380 4.5 53.5 1.0 PA A:ATP601 4.5 66.0 1.0 CB A:GLN422 4.6 53.5 1.0 O2B A:ATP601 4.6 66.0 1.0 O3A A:ATP601 4.7 66.0 1.0 N A:GLN422 4.7 53.5 1.0 O A:LYS380 4.8 53.5 1.0 N A:THR382 4.8 43.5 1.0 CA A:GLN422 5.0 53.5 1.0

Magnesium binding site 2 out of 2 in the Multidrug Resistance Transporter Bmra Mutant E504A Bound with Atp, Mg, and Rhodamine 6G Solved By Cryo-Em


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Multidrug Resistance Transporter Bmra Mutant E504A Bound with Atp, Mg, and Rhodamine 6G Solved By Cryo-Em within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg601 b:62.3 occ:1.00 O2B A:ATP602 1.9 64.0 1.0 OE1 B:GLN422 2.0 54.0 1.0 CB B:THR381 2.5 51.5 1.0 PB A:ATP602 2.5 64.0 1.0 O1B A:ATP602 2.6 64.0 1.0 CG2 B:THR381 2.7 51.5 1.0 CD B:GLN422 2.7 54.0 1.0 O1G A:ATP602 2.8 64.0 1.0 NE2 B:GLN422 2.8 54.0 1.0 OG1 B:THR381 3.5 51.5 1.0 PG A:ATP602 3.5 64.0 1.0 O3B A:ATP602 3.5 64.0 1.0 N B:THR381 3.5 51.5 1.0 O3G A:ATP602 3.5 64.0 1.0 CA B:THR381 3.6 51.5 1.0 O3A A:ATP602 4.0 64.0 1.0 CG B:GLN422 4.2 54.0 1.0 OG A:SER480 4.2 57.5 1.0 O1A A:ATP602 4.5 64.0 1.0 CB B:LYS380 4.6 51.9 1.0 PA A:ATP602 4.6 64.0 1.0 CB B:GLN422 4.7 54.0 1.0 CD1 B:ILE533 4.7 48.6 1.0 C B:LYS380 4.7 51.9 1.0 C B:THR381 4.8 51.5 1.0 OD1 B:ASP503 4.9 49.6 1.0 O2A A:ATP602 4.9 64.0 1.0 O2G A:ATP602 4.9 64.0 1.0

V.Chaptal, V.Zampieri, B.Wiseman, C.Orelle, J.Martin, K.A.Nguyen, A.Gobet, M.Di Cesare, S.Magnard, W.Javed, J.Eid, A.Kilburg, M.Peuchmaur, J.Marcoux, L.Monticelli, M.Hogbom, G.Schoehn, J.M.Jault, A.Boumendjel, P.Falson. Substrate-Bound and Substrate-Free Outward-Facing Structures of A Multidrug Abc Exporter. Sci Adv V. 8 G9215 2022.
ISSN: ESSN 2375-2548
PubMed: 35080979
DOI: 10.1126/SCIADV.ABG9215