Magnesium in PDB 7n4e: Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex

All present enzymatic activity of Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex:
2.7.7.6;

The structure of Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex (pdb code 7n4e). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex, PDB code: 7n4e:

Magnesium binding site 1 out of 1 in the Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1503 b:31.3 occ:1.00 OD1 D:ASP460 2.1 28.9 1.0 OD1 D:ASP462 2.1 27.9 1.0 OD1 D:ASP464 2.1 37.7 1.0 OD2 D:ASP460 2.1 28.9 1.0 OD2 D:ASP464 2.1 37.7 1.0 O3' R:A12 2.2 62.0 1.0 CG D:ASP460 2.3 28.9 1.0 CG D:ASP464 2.4 37.7 1.0 CG D:ASP462 3.0 27.9 1.0 OD2 D:ASP462 3.3 27.9 1.0 C3' R:A12 3.5 62.0 1.0 O2' R:A12 3.6 62.0 1.0 CB D:ASP460 3.8 28.9 1.0 CB D:ASP464 3.8 37.7 1.0 O D:ASP460 4.0 28.9 1.0 C4' R:A12 4.1 62.0 1.0 C2' R:A12 4.1 62.0 1.0 C D:ASP460 4.4 28.9 1.0 N D:ASP460 4.4 28.9 1.0 CA D:ASP460 4.4 28.9 1.0 CB D:ASP462 4.4 27.9 1.0 N D:ASP464 4.4 37.7 1.0 CA D:ASP464 4.5 37.7 1.0 N D:ASP462 4.5 27.9 1.0 C5' R:A12 4.8 62.0 1.0 CA D:ASP462 4.8 27.9 1.0 C D:ASP462 4.8 27.9 1.0 O D:ASP462 5.0 27.9 1.0

C.Pukhrambam, V.Molodtsov, M.Kooshkbaghi, A.Tareen, H.Vu, K.S.Skalenko, M.Su, Z.Yin, J.T.Winkelman, J.B.Kinney, R.H.Ebright, B.E.Nickels. Structural and Mechanistic Basis of Sigma-Dependent Transcriptional Pausing. Proc.Natl.Acad.Sci.Usa V. 119 01119 2022.
ISSN: ESSN 1091-6490
PubMed: 35653571
DOI: 10.1073/PNAS.2201301119