Magnesium in PDB 7use: Cryo-Em Structure of Wave Regulatory Complex with RAC1 Bound on Both A and D Site

All present enzymatic activity of Cryo-Em Structure of Wave Regulatory Complex with RAC1 Bound on Both A and D Site:
3.6.5.2;

The binding sites of Magnesium atom in the Cryo-Em Structure of Wave Regulatory Complex with RAC1 Bound on Both A and D Site (pdb code 7use). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cryo-Em Structure of Wave Regulatory Complex with RAC1 Bound on Both A and D Site, PDB code: 7use:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cryo-Em Structure of Wave Regulatory Complex with RAC1 Bound on Both A and D Site


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of Wave Regulatory Complex with RAC1 Bound on Both A and D Site within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg501 b:62.4 occ:1.00 CB F:THR17 2.6 64.2 1.0 O1B F:GNP500 2.8 62.6 1.0 CG2 F:THR17 2.9 64.2 1.0 OG1 F:THR35 3.0 59.4 1.0 OD2 F:ASP57 3.0 61.9 1.0 N3B F:GNP500 3.3 62.6 1.0 CG2 F:THR35 3.4 59.4 1.0 OG1 F:THR17 3.5 64.2 1.0 PB F:GNP500 3.6 62.6 1.0 CA F:THR17 3.7 64.2 1.0 O1G F:GNP500 3.7 62.6 1.0 N F:THR17 3.7 64.2 1.0 O3G F:GNP500 3.8 62.6 1.0 PG F:GNP500 3.8 62.6 1.0 CB F:THR35 3.8 59.4 1.0 CG F:ASP57 3.9 61.9 1.0 OD1 F:ASP57 4.0 61.9 1.0 O2A F:GNP500 4.4 62.6 1.0 O1A F:GNP500 4.4 62.6 1.0 O3A F:GNP500 4.5 62.6 1.0 N F:THR35 4.6 59.4 1.0 PA F:GNP500 4.6 62.6 1.0 O F:ILE33 4.7 59.2 1.0 O2B F:GNP500 4.8 62.6 1.0 CA F:THR35 4.9 59.4 1.0 C F:THR17 4.9 64.2 1.0 C F:LYS16 5.0 62.6 1.0

Magnesium binding site 2 out of 2 in the Cryo-Em Structure of Wave Regulatory Complex with RAC1 Bound on Both A and D Site


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of Wave Regulatory Complex with RAC1 Bound on Both A and D Site within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg501 b:14.7 occ:1.00 CG2 G:THR35 1.8 61.8 1.0 O3G G:GTP500 2.1 17.6 1.0 O1B G:GTP500 2.2 14.5 1.0 OG1 G:THR35 2.6 61.8 1.0 CB G:THR35 2.8 61.8 1.0 CG2 G:THR17 3.0 61.0 1.0 PG G:GTP500 3.4 15.4 1.0 PB G:GTP500 3.5 13.8 1.0 N G:THR35 3.5 61.8 1.0 O3B G:GTP500 3.6 12.2 1.0 CA G:THR35 3.8 61.8 1.0 O G:ILE33 3.9 62.8 1.0 O1A G:GTP500 4.0 19.0 1.0 OG1 G:THR17 4.1 61.0 1.0 C G:PRO34 4.1 61.1 1.0 CB G:THR17 4.2 61.0 1.0 O2G G:GTP500 4.2 16.2 1.0 O3A G:GTP500 4.3 15.1 1.0 OD1 G:ASP57 4.4 61.9 1.0 O1G G:GTP500 4.5 17.0 1.0 CA G:PRO34 4.6 61.1 1.0 O2B G:GTP500 4.6 12.9 1.0 PA G:GTP500 4.7 17.3 1.0 CE1 G:TYR32 4.8 61.5 1.0 O G:THR35 4.8 61.8 1.0 C G:THR35 4.9 61.8 1.0 O G:PRO34 4.9 61.1 1.0 C G:ILE33 4.9 62.8 1.0

B.Ding, S.Yang, M.Schaks, Y.Liu, A.J.Brown, K.Rottner, S.Chowdhury, B.Chen. Structures Reveal A Key Mechanism of Wave Regulatory Complex Activation By RAC1 Gtpase. Nat Commun V. 13 5444 2022.
ISSN: ESSN 2041-1723
PubMed: 36114192
DOI: 10.1038/S41467-022-33174-3