Magnesium in PDB 7uxc: Cryo-Em Structure of the MTORC1-Tfeb-Rag-Ragulator Complex with Symmetry Expansion

All present enzymatic activity of Cryo-Em Structure of the MTORC1-Tfeb-Rag-Ragulator Complex with Symmetry Expansion:
2.7.11.1;

The binding sites of Magnesium atom in the Cryo-Em Structure of the MTORC1-Tfeb-Rag-Ragulator Complex with Symmetry Expansion (pdb code 7uxc). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cryo-Em Structure of the MTORC1-Tfeb-Rag-Ragulator Complex with Symmetry Expansion, PDB code: 7uxc:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cryo-Em Structure of the MTORC1-Tfeb-Rag-Ragulator Complex with Symmetry Expansion


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of the MTORC1-Tfeb-Rag-Ragulator Complex with Symmetry Expansion within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg402 b:41.9 occ:1.00 O2B D:GTP401 2.3 40.7 1.0 CB D:THR21 2.5 31.1 1.0 O3G D:GTP401 2.6 40.7 1.0 H D:THR21 3.1 31.1 1.0 H D:THR42 3.1 39.8 1.0 CB D:THR42 3.4 39.8 1.0 OD2 D:ASP62 3.4 35.8 1.0 CA D:THR21 3.6 31.1 1.0 N D:THR21 3.6 31.1 1.0 OD1 D:ASP62 3.7 35.8 1.0 PB D:GTP401 3.7 40.7 1.0 O2A D:GTP401 3.7 40.7 1.0 PG D:GTP401 3.7 40.7 1.0 N D:THR42 3.7 39.8 1.0 HG3 D:LYS20 3.8 37.5 1.0 HG2 D:LYS20 3.9 37.5 1.0 CG D:ASP62 3.9 35.8 1.0 HA D:THR21 4.0 31.1 1.0 O1G D:GTP401 4.0 40.7 1.0 CA D:THR42 4.1 39.8 1.0 O3B D:GTP401 4.1 40.7 1.0 CG D:LYS20 4.3 37.5 1.0 HA D:ALA41 4.4 41.7 1.0 HA D:THR42 4.4 39.8 1.0 O D:GLY40 4.4 38.3 1.0 PA D:GTP401 4.6 40.7 1.0 HD2 D:LYS20 4.6 37.5 1.0 O3A D:GTP401 4.7 40.7 1.0 O1B D:GTP401 4.7 40.7 1.0 O1A D:GTP401 4.7 40.7 1.0 HH21 D:ARG24 4.7 25.7 1.0 HZ3 D:LYS20 4.7 37.5 1.0 HA2 D:GLY64 4.7 43.2 1.0 C D:ALA41 4.8 41.7 1.0 C D:THR21 4.8 31.1 1.0 C D:LYS20 4.9 37.5 1.0 HD13 D:LEU39 5.0 30.0 1.0 H D:SER22 5.0 34.4 1.0

Magnesium binding site 2 out of 2 in the Cryo-Em Structure of the MTORC1-Tfeb-Rag-Ragulator Complex with Symmetry Expansion


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of the MTORC1-Tfeb-Rag-Ragulator Complex with Symmetry Expansion within 5.0Å range: probe atom residue distance (Å) B Occ K:Mg402 b:57.6 occ:1.00 HG23 K:THR42 1.8 64.2 1.0 O3G K:GTP401 2.0 75.3 1.0 O2B K:GTP401 2.0 75.3 1.0 HG21 K:THR42 2.3 64.2 1.0 CG2 K:THR42 2.4 64.2 1.0 CB K:THR21 2.5 70.2 1.0 HG1 K:THR42 2.7 64.2 1.0 OG1 K:THR42 2.8 64.2 1.0 H K:THR42 3.0 64.2 1.0 PG K:GTP401 3.0 75.3 1.0 H K:THR21 3.1 70.2 1.0 CB K:THR42 3.2 64.2 1.0 PB K:GTP401 3.2 75.3 1.0 HG22 K:THR42 3.2 64.2 1.0 O1G K:GTP401 3.3 75.3 1.0 O2A K:GTP401 3.4 75.3 1.0 O3B K:GTP401 3.4 75.3 1.0 CA K:THR21 3.6 70.2 1.0 OD2 K:ASP62 3.6 68.8 1.0 N K:THR21 3.7 70.2 1.0 HG2 K:LYS20 3.7 75.0 1.0 N K:THR42 3.7 64.2 1.0 HB K:THR42 3.9 64.2 1.0 HA K:THR21 4.0 70.2 1.0 OD1 K:ASP62 4.0 68.8 1.0 CA K:THR42 4.0 64.2 1.0 O K:GLY40 4.1 60.0 1.0 O3A K:GTP401 4.2 75.3 1.0 PA K:GTP401 4.2 75.3 1.0 CG K:ASP62 4.2 68.8 1.0 HA K:ALA41 4.3 56.1 1.0 O1B K:GTP401 4.3 75.3 1.0 O2G K:GTP401 4.3 75.3 1.0 HZ3 K:LYS20 4.3 75.0 1.0 HG3 K:LYS20 4.4 75.0 1.0 O1A K:GTP401 4.4 75.3 1.0 CG K:LYS20 4.5 75.0 1.0 HA K:THR42 4.6 64.2 1.0 HH21 K:ARG24 4.7 73.1 1.0 HD2 K:LYS20 4.7 75.0 1.0 C K:ALA41 4.8 56.1 1.0 HA2 K:GLY64 4.8 72.5 1.0 C K:THR21 4.8 70.2 1.0 HZ2 K:LYS20 4.9 75.0 1.0 C K:LYS20 4.9 75.0 1.0 CA K:ALA41 4.9 56.1 1.0 H K:SER22 5.0 76.1 1.0

Z.Cui, J.Hurley. Structural Basis For MTORC1-Dependent Regulation of the Lysosomal and Autophagic Transcription Factor Tfeb To Be Published.