Magnesium in PDB 8bzf: Fc-J Acetonide Stabilizer of 14-3-3 and Eralpha

The structure of Fc-J Acetonide Stabilizer of 14-3-3 and Eralpha, PDB code: 8bzf was solved by E.J.Visser, C.Ottmann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.39 / 1.53
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	81.85, 112.086, 62.43, 90, 90, 90
R / Rfree (%)	18.3 / 21.4

The binding sites of Magnesium atom in the Fc-J Acetonide Stabilizer of 14-3-3 and Eralpha (pdb code 8bzf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Fc-J Acetonide Stabilizer of 14-3-3 and Eralpha, PDB code: 8bzf:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Fc-J Acetonide Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Fc-J Acetonide Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:35.0 occ:1.00 O A:HOH434 3.1 22.4 1.0 O A:HOH508 3.4 39.5 1.0 O A:HOH633 3.4 37.6 1.0 HZ1 A:LYS159 3.9 51.3 1.0 HZ3 A:LYS159 4.2 51.3 1.0 NZ A:LYS159 4.4 42.8 1.0 O A:HOH670 4.5 29.7 1.0 O A:HOH629 4.5 33.3 1.0 HZ2 A:LYS159 4.5 51.3 1.0 O A:HOH563 4.6 34.1 1.0 OE2 A:GLU189 4.7 20.5 1.0 O A:HOH591 4.7 37.0 1.0 O A:HOH642 4.7 43.2 1.0

Magnesium binding site 2 out of 3 in the Fc-J Acetonide Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Fc-J Acetonide Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:21.3 occ:0.73 O A:HOH550 2.1 32.0 1.0 OE1 A:GLU2 2.2 20.7 1.0 O A:HOH427 2.5 23.8 1.0 CD A:GLU2 3.2 23.8 1.0 OE2 A:GLU2 3.6 21.5 1.0 HA A:GLU2 4.0 18.4 1.0 H A:ARG3 4.2 20.1 1.0 O A:HOH570 4.2 23.2 1.0 O A:HOH406 4.4 46.0 1.0 CG A:GLU2 4.5 18.6 1.0 HB3 A:GLU2 4.6 20.1 1.0 O A:HOH461 4.6 24.3 1.0 O A:HOH455 4.7 29.4 1.0 HG2 A:GLU2 4.8 22.4 1.0 CA A:GLU2 4.8 15.4 1.0 CB A:GLU2 4.9 16.8 1.0 N A:ARG3 5.0 16.7 1.0

Magnesium binding site 3 out of 3 in the Fc-J Acetonide Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Fc-J Acetonide Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:26.8 occ:1.00 O A:HOH457 2.0 32.7 1.0 O A:HOH631 2.1 34.2 1.0 OE2 A:GLU89 2.3 23.6 1.0 O A:HOH597 2.5 39.4 1.0 CD A:GLU89 3.4 19.1 1.0 HG2 A:GLU86 3.5 27.5 1.0 HG2 A:GLU89 3.7 25.5 1.0 HH12 A:ARG85 3.9 29.7 1.0 HE22 A:GLN93 4.0 28.0 1.0 CG A:GLU89 4.1 21.2 1.0 O A:HOH504 4.1 38.3 1.0 HB3 A:GLU89 4.2 22.0 1.0 HH11 A:ARG85 4.2 29.7 1.0 OE1 A:GLU89 4.3 19.4 1.0 HA A:GLU86 4.4 23.1 1.0 O A:HOH611 4.4 37.1 1.0 NH1 A:ARG85 4.4 24.7 1.0 HG1 A:THR90 4.4 27.4 1.0 CG A:GLU86 4.4 22.9 1.0 OE1 A:GLN93 4.5 21.2 1.0 O A:HOH603 4.5 35.9 1.0 NE2 A:GLN93 4.7 23.4 1.0 CB A:GLU89 4.8 18.4 1.0 HG3 A:GLU86 4.8 27.5 1.0 OG1 A:THR90 4.8 22.9 1.0 HB3 A:GLU86 4.9 24.9 1.0 O A:HOH419 4.9 42.0 1.0 HG3 A:GLU89 4.9 25.5 1.0 CD A:GLN93 5.0 23.2 1.0

E.J.Visser, M.Donaldson Collier, J.Siefert, M.Konstantinidou, S.Paul, P.Cossar, G.Miley, O.Meijer, M.R.Arkin, C.Ottmann, W.Zwart, L.Brunsveld. Stabilization of the Estrogen Receptor Alpha - 14-3-3 Interaction As A Potential Intervention Strategy For Endocrine Resistance in Breast Cancer To Be Published.