Magnesium in PDB 8bzg: Fc-31 Stabilizer of 14-3-3 and Eralpha

The structure of Fc-31 Stabilizer of 14-3-3 and Eralpha, PDB code: 8bzg was solved by E.J.Visser, C.Ottmann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.45 / 1.49
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	82.097, 111.677, 62.546, 90, 90, 90
R / Rfree (%)	18 / 18.9

The binding sites of Magnesium atom in the Fc-31 Stabilizer of 14-3-3 and Eralpha (pdb code 8bzg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Fc-31 Stabilizer of 14-3-3 and Eralpha, PDB code: 8bzg:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Fc-31 Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Fc-31 Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:35.7 occ:1.00 O A:HOH436 3.1 19.4 1.0 O A:HOH620 3.5 36.1 1.0 O A:HOH515 3.6 34.7 1.0 HZ2 A:LYS159 4.2 59.8 1.0 O A:HOH663 4.4 30.4 1.0 HZ1 A:LYS159 4.4 59.8 1.0 HZ3 A:LYS159 4.5 59.8 1.0 NZ A:LYS159 4.6 49.8 1.0 O A:HOH624 4.6 32.1 1.0 O A:HOH565 4.6 32.8 1.0 O A:HOH628 4.7 36.4 1.0 OE2 A:GLU189 4.7 21.4 1.0

Magnesium binding site 2 out of 3 in the Fc-31 Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Fc-31 Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:24.3 occ:0.72 OE1 A:GLU2 2.3 20.1 1.0 O A:HOH424 2.5 23.8 1.0 CD A:GLU2 3.2 21.8 1.0 OE2 A:GLU2 3.6 22.3 1.0 HA A:GLU2 4.0 21.7 1.0 H A:ARG3 4.2 20.8 1.0 O A:HOH575 4.2 24.2 1.0 CG A:GLU2 4.5 19.3 1.0 HB3 A:GLU2 4.6 23.4 1.0 O A:HOH556 4.6 25.7 1.0 O A:HOH420 4.6 39.5 1.0 O A:HOH441 4.8 33.8 1.0 HG2 A:GLU2 4.8 23.2 1.0 CA A:GLU2 4.8 18.1 1.0 CB A:GLU2 4.9 19.4 1.0 N A:ARG3 5.0 17.4 1.0

Magnesium binding site 3 out of 3 in the Fc-31 Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Fc-31 Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:27.4 occ:1.00 O A:HOH627 2.2 36.9 1.0 O A:HOH500 2.2 42.5 1.0 O A:HOH442 2.3 32.1 1.0 O A:HOH478 2.3 38.4 1.0 O A:HOH665 2.4 45.1 1.0 OE2 A:GLU89 2.4 26.7 1.0 O A:HOH597 2.5 35.0 1.0 CD A:GLU89 3.4 21.9 1.0 HG2 A:GLU86 3.5 27.0 1.0 HG2 A:GLU89 3.7 27.6 1.0 HH12 A:ARG85 3.8 29.5 1.0 O A:HOH560 3.9 38.4 1.0 HE22 A:GLN93 4.0 28.6 1.0 CG A:GLU89 4.1 23.0 1.0 HH11 A:ARG85 4.2 29.5 1.0 O A:HOH588 4.2 36.0 1.0 HB3 A:GLU89 4.2 24.9 1.0 OE1 A:GLU89 4.3 20.0 1.0 NH1 A:ARG85 4.3 24.6 1.0 HA A:GLU86 4.3 23.1 1.0 CG A:GLU86 4.4 22.5 1.0 HG1 A:THR90 4.5 29.4 1.0 O A:HOH608 4.6 38.4 1.0 OE1 A:GLN93 4.6 24.1 1.0 NE2 A:GLN93 4.7 23.8 1.0 HB3 A:GLU86 4.8 22.6 1.0 CB A:GLU89 4.8 20.7 1.0 OG1 A:THR90 4.8 24.5 1.0 O A:HOH403 4.9 38.5 1.0 HG3 A:GLU86 4.9 27.0 1.0 HG3 A:GLU89 4.9 27.6 1.0

E.J.Visser, M.Donaldson Collier, J.Siefert, M.Konstantinidou, S.Paul, P.Cossar, G.Miley, O.Meijer, M.R.Arkin, C.Ottmann, W.Zwart, L.Brunsveld. Stabilization of the Estrogen Receptor Alpha - 14-3-3 Interaction As A Potential Intervention Strategy For Endocrine Resistance in Breast Cancer To Be Published.