Atomistry » Magnesium » PDB 8gxc-8h68 » 8h4c
Atomistry »
  Magnesium »
    PDB 8gxc-8h68 »
      8h4c »

Magnesium in PDB 8h4c: Blasnase-T13A/M57P

Protein crystallography data

The structure of Blasnase-T13A/M57P, PDB code: 8h4c was solved by F.Lu, W.Wang, H.Chi, T.Ran, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.83 / 1.60
Space group P 41 21 2
Cell size a, b, c (Å), α, β, γ (°) 91.716, 91.716, 233.017, 90, 90, 90
R / Rfree (%) 18.4 / 19.3

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Blasnase-T13A/M57P (pdb code 8h4c). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 7 binding sites of Magnesium where determined in the Blasnase-T13A/M57P, PDB code: 8h4c:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7;

Magnesium binding site 1 out of 7 in 8h4c

Go back to Magnesium Binding Sites List in 8h4c
Magnesium binding site 1 out of 7 in the Blasnase-T13A/M57P


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Blasnase-T13A/M57P within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg401

b:32.7
occ:0.00
 O A:HOH502 2.2 39.7 1.0
O A:HOH640 2.3 29.4 1.0
OE2 B:GLU207 2.3 35.8 1.0
OD2 A:ASP218 2.4 28.8 1.0
O A:HOH738 2.4 36.8 1.0
O A:HOH512 2.7 32.0 1.0
CD B:GLU207 3.1 36.2 1.0
CG A:ASP218 3.3 29.1 1.0
O1 A:FMT406 3.5 35.0 0.0
MG B:MG401 3.6 37.0 0.0
OE1 B:GLU207 3.7 39.4 1.0
NZ A:LYS220 3.8 26.1 1.0
O A:HOH612 3.9 36.6 1.0
CB A:ASP218 3.9 23.9 1.0
CG B:GLU207 4.0 32.9 1.0
OD1 A:ASP218 4.2 26.1 1.0
O B:HOH539 4.2 31.1 1.0
O A:HOH516 4.2 39.7 1.0
C A:FMT406 4.4 35.3 0.0
O A:HOH532 4.5 41.7 1.0
O2 B:FMT404 4.5 27.1 0.0
O A:HOH665 4.6 25.0 1.0
CE A:LYS220 4.8 26.3 1.0
CB B:GLU207 5.0 28.1 1.0
O A:ASP216 5.0 25.3 1.0

Magnesium binding site 2 out of 7 in 8h4c

Go back to Magnesium Binding Sites List in 8h4c
Magnesium binding site 2 out of 7 in the Blasnase-T13A/M57P


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Blasnase-T13A/M57P within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg402

b:45.7
occ:0.00
 O A:SER79 3.0 43.4 1.0
OD2 A:ASP81 3.6 43.1 1.0
C A:SER79 4.0 43.2 1.0
CG A:ASP81 4.2 44.1 1.0
OD1 A:ASP81 4.3 46.4 1.0
CA A:SER79 4.3 46.8 1.0
O A:LYS2 4.4 55.7 1.0
CB A:SER79 4.6 44.6 1.0
N A:LYS4 4.7 43.0 1.0
CA A:LYS3 4.9 45.2 1.0
CB A:LYS4 4.9 43.2 1.0

Magnesium binding site 3 out of 7 in 8h4c

Go back to Magnesium Binding Sites List in 8h4c
Magnesium binding site 3 out of 7 in the Blasnase-T13A/M57P


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Blasnase-T13A/M57P within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg403

b:29.8
occ:0.00
 O2 A:FMT405 2.2 29.0 0.0
O A:HOH633 2.3 35.4 1.0
O A:ASN246 2.4 25.0 1.0
O A:HOH568 2.4 33.2 1.0
OD2 A:ASP250 2.4 29.8 1.0
O A:HOH718 2.5 33.7 1.0
C A:FMT405 3.4 29.8 0.0
CG A:ASP250 3.4 27.6 1.0
C A:ASN246 3.5 25.8 1.0
OD1 A:ASP250 3.7 28.6 1.0
NH1 A:ARG223 3.8 28.1 1.0
O A:HOH753 4.2 43.7 1.0
CA A:ASN246 4.2 24.0 1.0
N A:GLY219 4.2 23.6 1.0
O1 A:FMT405 4.3 31.9 0.0
CB A:ASN246 4.3 23.5 1.0
O A:HOH725 4.3 39.1 1.0
O A:HOH666 4.3 28.4 1.0
CA A:GLY219 4.4 22.7 1.0
N A:MET247 4.6 22.7 1.0
O A:HOH696 4.6 44.2 1.0
CB A:ASP250 4.7 21.8 1.0
N A:ASP250 4.7 23.6 1.0
CA A:MET247 4.8 22.0 1.0

Magnesium binding site 4 out of 7 in 8h4c

Go back to Magnesium Binding Sites List in 8h4c
Magnesium binding site 4 out of 7 in the Blasnase-T13A/M57P


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Blasnase-T13A/M57P within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg404

b:23.8
occ:0.00
 O A:HOH715 2.2 31.1 1.0
O A:HOH596 2.4 22.8 1.0
O A:HOH551 2.4 31.9 1.0
O A:ASP295 2.5 21.8 1.0
O A:HOH736 2.6 26.5 1.0
O A:GLU268 2.7 23.5 1.0
C A:ASP295 3.5 21.8 1.0
C A:GLU268 3.6 21.5 1.0
O A:HOH634 3.8 28.2 1.0
O A:ALA267 4.1 22.0 1.0
CB A:ASP295 4.1 21.4 1.0
CA A:ASP295 4.2 20.4 1.0
O A:HOH598 4.2 27.2 1.0
N A:GLU269 4.2 20.4 1.0
CA A:GLU269 4.3 19.0 1.0
N A:ASP297 4.4 20.9 1.0
C A:TYR296 4.5 21.2 1.0
CA A:GLU268 4.5 21.3 1.0
N A:TYR296 4.6 21.3 1.0
O A:HOH581 4.7 35.3 1.0
O A:GLY270 4.7 22.1 1.0
C A:GLU269 4.7 19.2 1.0
CD1 A:TYR159 4.7 21.6 1.0
CA A:TYR296 4.8 20.5 1.0
CE1 A:TYR159 4.8 23.8 1.0
CG A:ASP295 4.8 23.5 1.0
O A:TYR296 4.9 23.1 1.0
N A:GLY270 4.9 20.7 1.0

Magnesium binding site 5 out of 7 in 8h4c

Go back to Magnesium Binding Sites List in 8h4c
Magnesium binding site 5 out of 7 in the Blasnase-T13A/M57P


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Blasnase-T13A/M57P within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg401

b:37.0
occ:0.00
 O A:HOH612 2.4 36.6 1.0
O A:HOH738 2.5 36.8 1.0
OE1 B:GLU207 2.6 39.4 1.0
O B:GLU228 2.6 38.5 1.0
OE2 B:GLU207 2.7 35.8 1.0
CD B:GLU207 3.1 36.2 1.0
MG A:MG401 3.6 32.7 0.0
C B:GLU228 3.8 32.8 1.0
CB B:GLU228 3.8 32.9 1.0
OD2 A:ASP218 4.0 28.8 1.0
CE A:LYS220 4.0 26.3 1.0
O B:HOH503 4.1 42.7 1.0
NZ A:LYS220 4.2 26.1 1.0
O A:HOH532 4.3 41.7 1.0
CA B:GLU228 4.3 33.0 1.0
O A:HOH502 4.4 39.7 1.0
CG B:GLU207 4.6 32.9 1.0
N B:GLY229 4.8 29.6 1.0
OE2 B:GLU228 4.9 48.4 1.0
O A:HOH516 4.9 39.7 1.0
CG B:GLU228 5.0 37.0 1.0

Magnesium binding site 6 out of 7 in 8h4c

Go back to Magnesium Binding Sites List in 8h4c
Magnesium binding site 6 out of 7 in the Blasnase-T13A/M57P


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Blasnase-T13A/M57P within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg402

b:26.1
occ:0.00
 O B:HOH751 2.2 33.4 1.0
O B:ASP295 2.3 21.5 1.0
O B:HOH653 2.3 25.5 1.0
O B:HOH761 2.5 29.9 1.0
O B:HOH568 2.6 34.5 1.0
O B:GLU268 2.9 26.5 1.0
C B:ASP295 3.4 24.7 1.0
C B:GLU268 3.8 23.6 1.0
O B:HOH637 3.9 29.3 1.0
CB B:ASP295 3.9 25.2 1.0
CA B:ASP295 4.0 24.6 1.0
O B:ALA267 4.2 24.6 1.0
O B:HOH586 4.2 26.3 1.0
CA B:GLU269 4.3 22.1 1.0
N B:GLU269 4.3 21.3 1.0
N B:ASP297 4.4 19.8 1.0
N B:TYR296 4.5 24.5 1.0
C B:TYR296 4.5 24.1 1.0
O B:GLY270 4.6 25.0 1.0
CG B:ASP295 4.7 26.2 1.0
CD1 B:TYR159 4.7 24.8 1.0
CA B:GLU268 4.7 24.6 1.0
CE1 B:TYR159 4.7 23.4 1.0
C B:GLU269 4.7 22.3 1.0
CA B:TYR296 4.8 22.6 1.0
O B:HOH578 4.9 34.8 1.0
O B:TYR296 4.9 26.1 1.0
N B:GLY270 4.9 24.5 1.0

Magnesium binding site 7 out of 7 in 8h4c

Go back to Magnesium Binding Sites List in 8h4c
Magnesium binding site 7 out of 7 in the Blasnase-T13A/M57P


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Blasnase-T13A/M57P within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg403

b:32.7
occ:0.00
 O B:HOH763 2.2 39.5 1.0
O B:HOH696 2.4 42.5 1.0
O2 B:FMT405 2.4 31.4 0.0
O B:HOH618 2.4 36.5 1.0
O B:ASN246 2.4 30.0 1.0
OD2 B:ASP250 2.4 31.1 1.0
C B:FMT405 3.3 33.2 0.0
CG B:ASP250 3.4 29.4 1.0
C B:ASN246 3.6 30.5 1.0
OD1 B:ASP250 3.8 31.8 1.0
NH1 B:ARG223 3.8 28.6 1.0
N B:GLY219 4.2 22.9 1.0
CA B:ASN246 4.3 29.0 1.0
O1 B:FMT405 4.3 33.8 0.0
CB B:ASN246 4.4 27.0 1.0
O B:HOH676 4.5 32.5 1.0
CA B:GLY219 4.5 24.5 1.0
N B:MET247 4.6 27.7 1.0
CB B:ASP250 4.7 28.0 1.0
CA B:MET247 4.8 26.5 1.0
N B:ASP250 4.8 28.4 1.0

Reference:

F.Lu, W.Wang, H.Chi, T.Ran. Structure-Based Rational Design of Bacillus Licheniformis L-Asparaginase with Low/No D-Asparaginase Activity For A Safer Enzyme To Be Published.
Page generated: Fri Aug 15 06:05:03 2025

Last articles

Mg in 8IS8
Mg in 8IS7
Mg in 8IRG
Mg in 8IRF
Mg in 8IRE
Mg in 8IRD
Mg in 8IRB
Mg in 8IRA
Mg in 8IRC
Mg in 8IR9
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy