Magnesium in PDB 8hkc: Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex

All present enzymatic activity of Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex:
2.7.7.6;

The structure of Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex (pdb code 8hkc). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex, PDB code: 8hkc:

Magnesium binding site 1 out of 1 in the Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg1503 b:37.6 occ:1.00 OD1 F:ASP460 2.1 37.6 1.0 OD1 F:ASP462 2.1 37.6 1.0 OD2 F:ASP464 2.9 35.9 1.0 CG F:ASP462 2.9 37.6 1.0 OD1 F:ASP464 3.0 35.9 1.0 CG F:ASP460 3.0 37.6 1.0 OD2 F:ASP462 3.0 37.6 1.0 OD2 F:ASP460 3.2 37.6 1.0 CG F:ASP464 3.2 35.9 1.0 O F:ASP460 3.9 37.6 1.0 CB F:ASP460 4.4 37.6 1.0 CB F:ASP462 4.4 37.6 1.0 C F:ASP460 4.5 37.6 1.0 N F:ASP460 4.6 37.6 1.0 CB F:ASP464 4.6 35.9 1.0 N F:ASP462 4.7 37.6 1.0 CA F:ASP460 4.7 37.6 1.0 N F:ASP464 4.8 35.9 1.0 C F:ASP462 4.8 37.6 1.0 NH2 F:ARG425 4.9 16.6 1.0 CA F:ASP462 4.9 37.6 1.0

Q.Lu, T.Chen, J.Wang, F.Wang, W.Ye, L.Ma, S.Wu. Structural Insight Into the Mechanism of Sigma 32-Mediated Transcription Initiation of Bacterial Rna Polymerase Biomolecules V. 13 2023.
ISSN: ESSN 2218-273X
DOI: 10.3390/BIOM13050738