Magnesium in PDB 8sb9: Cryoem Structure of P-Glycoprotein in Inward Facing 1 State Under Continuous Turnover Conditions with Vinblastine

All present enzymatic activity of Cryoem Structure of P-Glycoprotein in Inward Facing 1 State Under Continuous Turnover Conditions with Vinblastine:
7.6.2.1; 7.6.2.2;

The binding sites of Magnesium atom in the Cryoem Structure of P-Glycoprotein in Inward Facing 1 State Under Continuous Turnover Conditions with Vinblastine (pdb code 8sb9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cryoem Structure of P-Glycoprotein in Inward Facing 1 State Under Continuous Turnover Conditions with Vinblastine, PDB code: 8sb9:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cryoem Structure of P-Glycoprotein in Inward Facing 1 State Under Continuous Turnover Conditions with Vinblastine


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryoem Structure of P-Glycoprotein in Inward Facing 1 State Under Continuous Turnover Conditions with Vinblastine within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1302 b:130.1 occ:1.00 HE22 A:GLN1118 1.8 118.4 1.0 O1G A:ATP1301 1.9 131.3 1.0 O2B A:ATP1301 2.0 131.3 1.0 OE1 A:GLN1118 2.1 118.4 1.0 NE2 A:GLN1118 2.2 118.4 1.0 CD A:GLN1118 2.4 118.4 1.0 OG A:SER1077 2.6 125.3 1.0 O2A A:ATP1301 2.6 131.3 1.0 PG A:ATP1301 2.9 131.3 1.0 HE21 A:GLN1118 3.0 118.4 1.0 PB A:ATP1301 3.0 131.3 1.0 O3B A:ATP1301 3.1 131.3 1.0 O3A A:ATP1301 3.5 131.3 1.0 HB2 A:SER1077 3.5 125.3 1.0 H A:SER1077 3.5 125.3 1.0 O2G A:ATP1301 3.5 131.3 1.0 CB A:SER1077 3.6 125.3 1.0 PA A:ATP1301 3.7 131.3 1.0 CG A:GLN1118 3.8 118.4 1.0 HG3 A:GLN1118 3.9 118.4 1.0 N A:SER1077 4.2 125.3 1.0 O3G A:ATP1301 4.2 131.3 1.0 HB3 A:SER1077 4.3 125.3 1.0 HG2 A:GLN1118 4.3 118.4 1.0 O1B A:ATP1301 4.3 131.3 1.0 HB2 A:GLN1118 4.5 118.4 1.0 CA A:SER1077 4.5 125.3 1.0 O1A A:ATP1301 4.7 131.3 1.0 CB A:GLN1118 4.7 118.4 1.0 O5' A:ATP1301 4.7 131.3 1.0 HA A:SER1077 4.8 125.3 1.0 HB3 A:GLN1118 4.9 118.4 1.0

Magnesium binding site 2 out of 2 in the Cryoem Structure of P-Glycoprotein in Inward Facing 1 State Under Continuous Turnover Conditions with Vinblastine


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryoem Structure of P-Glycoprotein in Inward Facing 1 State Under Continuous Turnover Conditions with Vinblastine within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1303 b:106.1 occ:1.00 O2B A:ATP1306 1.9 106.3 1.0 OG A:SER434 1.9 96.5 1.0 O2G A:ATP1306 1.9 106.3 1.0 O3B A:ATP1306 2.7 106.3 1.0 PB A:ATP1306 2.8 106.3 1.0 PG A:ATP1306 2.9 106.3 1.0 H A:SER434 3.2 96.5 1.0 CB A:SER434 3.3 96.5 1.0 HB2 A:SER434 3.6 96.5 1.0 O1B A:ATP1306 3.7 106.3 1.0 O3G A:ATP1306 3.7 106.3 1.0 HB3 A:SER434 3.8 96.5 1.0 N A:SER434 3.8 96.5 1.0 NE2 A:GLN475 3.9 100.9 1.0 O3A A:ATP1306 4.0 106.3 1.0 O1G A:ATP1306 4.0 106.3 1.0 CA A:SER434 4.0 96.5 1.0 HG21 A:ILE585 4.1 111.3 1.0 HA A:SER434 4.2 96.5 1.0 OE1 A:GLN475 4.4 100.9 1.0 CD A:GLN475 4.5 100.9 1.0 O2A A:ATP1306 4.6 106.3 1.0 CB A:LYS433 4.9 103.0 1.0 C A:LYS433 4.9 103.0 1.0 PA A:ATP1306 4.9 106.3 1.0 HG22 A:ILE585 4.9 111.3 1.0 CG2 A:ILE585 4.9 111.3 1.0

A.T.Culbertson, M.Liao. Cryo-Em of Human P-Glycoprotein Reveals An Intermediate Occluded Conformation During Active Drug Transport. Nat Commun V. 16 3619 2025.
ISSN: ESSN 2041-1723
PubMed: 40240353
DOI: 10.1038/S41467-025-58561-4