Magnesium in PDB 8tw7: Cryo-Em Structure of S. Cerevisiae CTF18-Rfc-Pcna Complex in Apo State Conformation I

The binding sites of Magnesium atom in the Cryo-Em Structure of S. Cerevisiae CTF18-Rfc-Pcna Complex in Apo State Conformation I (pdb code 8tw7). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Cryo-Em Structure of S. Cerevisiae CTF18-Rfc-Pcna Complex in Apo State Conformation I, PDB code: 8tw7:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Cryo-Em Structure of S. Cerevisiae CTF18-Rfc-Pcna Complex in Apo State Conformation I


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of S. Cerevisiae CTF18-Rfc-Pcna Complex in Apo State Conformation I within 5.0Å range: probe atom residue distance (Å) B Occ 4:Mg402 b:35.1 occ:1.00 O3G 4:AGS401 2.1 40.3 1.0 OG1 4:THR56 2.2 35.9 1.0 O1B 4:AGS401 3.2 40.3 1.0 CB 4:THR56 3.2 35.9 1.0 PG 4:AGS401 3.6 40.3 1.0 O1A 4:AGS401 3.7 40.3 1.0 CG2 4:THR56 4.0 35.9 1.0 OE1 4:GLU115 4.0 37.4 1.0 S1G 4:AGS401 4.1 40.3 1.0 PB 4:AGS401 4.3 40.3 1.0 NH1 3:ARG131 4.4 31.1 1.0 O3B 4:AGS401 4.4 40.3 1.0 CA 4:THR56 4.5 35.9 1.0 N 4:THR56 4.5 35.9 1.0 O2G 4:AGS401 4.5 40.3 1.0 PA 4:AGS401 4.6 40.3 1.0 OD1 4:ASP114 4.6 36.1 1.0 O3A 4:AGS401 4.7 40.3 1.0 NH2 3:ARG131 4.8 31.1 1.0 O2A 4:AGS401 4.9 40.3 1.0 CG 4:ASP114 5.0 36.1 1.0 CZ 3:ARG131 5.0 31.1 1.0

Magnesium binding site 2 out of 3 in the Cryo-Em Structure of S. Cerevisiae CTF18-Rfc-Pcna Complex in Apo State Conformation I


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of S. Cerevisiae CTF18-Rfc-Pcna Complex in Apo State Conformation I within 5.0Å range: probe atom residue distance (Å) B Occ 3:Mg402 b:34.0 occ:1.00 O3G 3:AGS401 2.1 48.5 1.0 OG1 3:THR60 2.2 31.2 1.0 O1B 3:AGS401 2.8 48.5 1.0 CB 3:THR60 3.4 31.2 1.0 PG 3:AGS401 3.4 48.5 1.0 OD2 3:ASP117 3.4 28.1 1.0 S1G 3:AGS401 3.7 48.5 1.0 OD1 3:ASP117 3.7 28.1 1.0 PB 3:AGS401 4.0 48.5 1.0 CG 3:ASP117 4.0 28.1 1.0 O1A 3:AGS401 4.0 48.5 1.0 CG2 3:THR60 4.1 31.2 1.0 O3B 3:AGS401 4.1 48.5 1.0 OE2 3:GLU118 4.2 29.8 1.0 NH2 3:ARG206 4.3 29.1 1.0 CA 3:THR60 4.5 31.2 1.0 O2G 3:AGS401 4.5 48.5 1.0 N 3:THR60 4.6 31.2 1.0 O3A 3:AGS401 4.6 48.5 1.0 PA 3:AGS401 4.7 48.5 1.0 CG 3:GLU118 4.7 29.8 1.0 CD2 3:LEU146 4.8 25.0 1.0 O2A 3:AGS401 4.9 48.5 1.0 CD 3:GLU118 4.9 29.8 1.0

Magnesium binding site 3 out of 3 in the Cryo-Em Structure of S. Cerevisiae CTF18-Rfc-Pcna Complex in Apo State Conformation I


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Cryo-Em Structure of S. Cerevisiae CTF18-Rfc-Pcna Complex in Apo State Conformation I within 5.0Å range: probe atom residue distance (Å) B Occ 2:Mg402 b:34.1 occ:1.00 O3G 2:AGS401 2.2 48.5 1.0 OG1 2:THR72 2.3 29.4 1.0 O2B 2:AGS401 2.4 48.5 1.0 OD1 2:ASP140 3.4 29.0 1.0 PG 2:AGS401 3.4 48.5 1.0 CB 2:THR72 3.5 29.4 1.0 OD2 2:ASP140 3.7 29.0 1.0 S1G 2:AGS401 3.8 48.5 1.0 PB 2:AGS401 3.8 48.5 1.0 CG 2:ASP140 3.9 29.0 1.0 OE2 2:GLU141 4.0 30.0 1.0 O2A 2:AGS401 4.0 48.5 1.0 O3B 2:AGS401 4.1 48.5 1.0 N 2:THR72 4.4 29.4 1.0 CG2 2:THR72 4.4 29.4 1.0 CA 2:THR72 4.5 29.4 1.0 CG2 2:ILE169 4.6 20.8 1.0 O2G 2:AGS401 4.6 48.5 1.0 O1B 2:AGS401 4.6 48.5 1.0 PA 2:AGS401 4.8 48.5 1.0 O3A 2:AGS401 4.8 48.5 1.0 O1A 2:AGS401 4.8 48.5 1.0 CD 2:GLU141 4.9 30.0 1.0

Z.Yuan, R.Georgescu, M.O'donnell, H.Li. Cryo-Em Reveals That CTF18-Rfc Collaborates with Pole to Load the Pcna Clamp Onto A Leading Strand Dna To Be Published.