Magnesium in PDB 9f3h: Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin

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>>> Page 1 <<< Page 2, Binding sites: 11 - 12;

Binding sites:

The binding sites of Magnesium atom in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin (pdb code 9f3h). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 12 binding sites of Magnesium where determined in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin, PDB code: 9f3h:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Magnesium binding site 1 out of 12 in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:88.5 occ:1.00 O3B A:GTP501 2.0 84.1 1.0 O1B A:GTP501 2.2 84.1 1.0 OE1 A:GLU71 2.3 87.3 1.0 PB A:GTP501 2.4 84.1 1.0 OE2 A:GLU71 2.4 87.3 1.0 CD A:GLU71 2.5 87.3 1.0 PG A:GTP501 2.9 84.1 1.0 O3G A:GTP501 3.0 84.1 1.0 O2B A:GTP501 3.0 84.1 1.0 CB A:ASP98 3.4 93.1 1.0 O2G A:GTP501 3.5 84.1 1.0 OD2 A:ASP98 3.7 93.1 1.0 CG A:ASP98 3.8 93.1 1.0 CG A:GLU71 3.8 87.3 1.0 O3A A:GTP501 3.8 84.1 1.0 O1G A:GTP501 4.2 84.1 1.0 NZ N:LYS252 4.4 83.1 1.0 CB A:GLU71 4.5 87.3 1.0 OD1 A:ASP98 4.6 93.1 1.0 CG2 A:THR145 4.7 79.8 1.0 CD N:LYS252 4.8 83.1 1.0 CA A:ASP98 4.8 93.1 1.0

Magnesium binding site 2 out of 12 in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:68.1 occ:1.00 O3B B:GTP501 2.1 69.5 1.0 PB B:GTP501 2.4 69.5 1.0 O1B B:GTP501 2.4 69.5 1.0 O2B B:GTP501 2.6 69.5 1.0 OE2 B:GLU69 3.2 69.7 1.0 PG B:GTP501 3.5 69.5 1.0 O3A B:GTP501 3.9 69.5 1.0 O2G B:GTP501 4.0 69.5 1.0 CB B:GLU69 4.0 69.7 1.0 O3G B:GTP501 4.1 69.5 1.0 CG2 B:THR143 4.2 70.0 1.0 OD2 B:ASP67 4.3 67.1 1.0 CD B:GLU69 4.3 69.7 1.0 OD1 B:ASP67 4.4 67.1 1.0 O1G B:GTP501 4.6 69.5 1.0 CG B:GLU69 4.6 69.7 1.0 CA B:GLY96 4.7 68.1 1.0 CG B:ASP67 4.8 67.1 1.0 OE1 B:GLN11 5.0 58.4 1.0

Magnesium binding site 3 out of 12 in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg502 b:43.4 occ:1.00 O1B G:GTP501 2.0 63.7 1.0 O3B G:GTP501 2.0 63.7 1.0 PB G:GTP501 2.4 63.7 1.0 O3G G:GTP501 2.5 63.7 1.0 PG G:GTP501 2.7 63.7 1.0 OE2 G:GLU71 3.1 69.0 1.0 O2B G:GTP501 3.3 63.7 1.0 O2G G:GTP501 3.4 63.7 1.0 CB G:ASP98 3.4 71.9 1.0 O3A G:GTP501 3.7 63.7 1.0 CG G:ASP98 3.7 71.9 1.0 OD2 G:ASP98 3.8 71.9 1.0 CD G:GLU71 4.0 69.0 1.0 O1G G:GTP501 4.0 63.7 1.0 NZ B:LYS252 4.3 58.4 1.0 OD2 G:ASP69 4.4 62.2 1.0 CG2 G:THR145 4.4 58.7 1.0 OD1 G:ASP98 4.5 71.9 1.0 CB G:GLU71 4.5 69.0 1.0 OE1 G:GLU71 4.5 69.0 1.0 OD1 G:ASP69 4.7 62.2 1.0 CA G:ASP98 4.8 71.9 1.0 CG G:GLU71 4.9 69.0 1.0

Magnesium binding site 4 out of 12 in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ N:Mg502 b:115.4 occ:1.00 OE2 N:GLU69 2.1 107.1 1.0 O3B N:GTP501 2.1 108.2 1.0 PB N:GTP501 2.4 108.2 1.0 O1B N:GTP501 2.5 108.2 1.0 O2B N:GTP501 2.5 108.2 1.0 CD N:GLU69 2.9 107.1 1.0 OE1 N:GLU69 3.4 107.1 1.0 PG N:GTP501 3.4 108.2 1.0 O3G N:GTP501 3.7 108.2 1.0 O2G N:GTP501 3.9 108.2 1.0 O3A N:GTP501 4.0 108.2 1.0 CG N:GLU69 4.0 107.1 1.0 CG2 N:THR143 4.3 103.7 1.0 OD1 N:ASP67 4.5 102.9 1.0 OE1 N:GLN11 4.6 93.9 1.0 O1G N:GTP501 4.6 108.2 1.0 OD2 N:ASP67 4.7 102.9 1.0 CA N:GLY96 4.8 101.0 1.0 CB N:GLU69 4.9 107.1 1.0

Magnesium binding site 5 out of 12 in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg502 b:81.8 occ:1.00 O3B C:GTP501 2.0 80.1 1.0 OE2 C:GLU71 2.0 80.7 1.0 O1B C:GTP501 2.0 80.1 1.0 O3G C:GTP501 2.1 80.1 1.0 PG C:GTP501 2.3 80.1 1.0 PB C:GTP501 2.4 80.1 1.0 CD C:GLU71 2.9 80.7 1.0 O2G C:GTP501 3.0 80.1 1.0 O2B C:GTP501 3.4 80.1 1.0 OE1 C:GLU71 3.5 80.7 1.0 O3A C:GTP501 3.7 80.1 1.0 O1G C:GTP501 3.7 80.1 1.0 CB C:ASP98 3.8 88.3 1.0 CG C:GLU71 4.0 80.7 1.0 CG C:ASP98 4.2 88.3 1.0 CG2 C:THR145 4.3 72.9 1.0 NZ P:LYS252 4.3 91.4 1.0 OD2 C:ASP98 4.5 88.3 1.0 NE2 C:GLN11 4.7 70.3 1.0 OD1 C:ASP69 4.8 79.2 1.0 OD1 C:ASP98 4.9 88.3 1.0 CD C:GLN11 4.9 70.3 1.0 CB C:GLU71 5.0 80.7 1.0

Magnesium binding site 6 out of 12 in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg502 b:77.1 occ:1.00 O3B D:GTP501 2.0 75.8 1.0 OE2 D:GLU69 2.1 75.5 1.0 O1B D:GTP501 2.2 75.8 1.0 PB D:GTP501 2.4 75.8 1.0 CD D:GLU69 3.0 75.5 1.0 O2B D:GTP501 3.1 75.8 1.0 PG D:GTP501 3.3 75.8 1.0 OE1 D:GLU69 3.5 75.5 1.0 O3G D:GTP501 3.7 75.8 1.0 O2G D:GTP501 3.8 75.8 1.0 O3A D:GTP501 3.9 75.8 1.0 CG D:GLU69 4.1 75.5 1.0 OD2 D:ASP67 4.1 73.3 1.0 CG2 D:THR143 4.4 70.9 1.0 O1G D:GTP501 4.5 75.8 1.0 OD1 D:ASP67 4.6 73.3 1.0 CB D:GLU69 4.6 75.5 1.0 OE1 D:GLN11 4.6 66.8 1.0 CG D:ASP67 4.8 73.3 1.0 CA D:GLY96 4.9 71.3 1.0 CB D:GLN11 5.0 66.8 1.0

Magnesium binding site 7 out of 12 in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ I:Mg502 b:50.2 occ:1.00 O1B I:GTP501 1.9 74.9 1.0 O3B I:GTP501 2.1 74.9 1.0 O3G I:GTP501 2.1 74.9 1.0 PB I:GTP501 2.4 74.9 1.0 PG I:GTP501 2.4 74.9 1.0 OE2 I:GLU71 2.7 71.8 1.0 O2G I:GTP501 3.1 74.9 1.0 CB I:ASP98 3.2 76.2 1.0 O2B I:GTP501 3.4 74.9 1.0 O3A I:GTP501 3.7 74.9 1.0 CD I:GLU71 3.7 71.8 1.0 CG I:ASP98 3.7 76.2 1.0 O1G I:GTP501 3.8 74.9 1.0 OD1 I:ASP98 3.9 76.2 1.0 CG2 I:THR145 4.2 61.2 1.0 NZ D:LYS252 4.3 62.1 1.0 OE1 I:GLU71 4.4 71.8 1.0 OD2 I:ASP98 4.5 76.2 1.0 CG I:GLU71 4.5 71.8 1.0 CA I:ASP98 4.6 76.2 1.0 OD1 I:ASP69 4.6 68.5 1.0 CB I:GLU71 4.7 71.8 1.0 OD2 I:ASP69 4.8 68.5 1.0

Magnesium binding site 8 out of 12 in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 8 of Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ P:Mg502 b:121.2 occ:1.00 OE2 P:GLU69 2.0 113.1 1.0 O3B P:GTP501 2.0 110.7 1.0 O1B P:GTP501 2.1 110.7 1.0 PB P:GTP501 2.4 110.7 1.0 CD P:GLU69 2.9 113.1 1.0 PG P:GTP501 2.9 110.7 1.0 O3G P:GTP501 3.0 110.7 1.0 O2B P:GTP501 3.0 110.7 1.0 OE1 P:GLU69 3.2 113.1 1.0 O2G P:GTP501 3.5 110.7 1.0 O3A P:GTP501 3.8 110.7 1.0 CG P:GLU69 4.3 113.1 1.0 O1G P:GTP501 4.3 110.7 1.0 CA P:GLY96 4.7 112.9 1.0 OD2 P:ASP67 4.7 110.7 1.0 CG2 P:THR143 4.8 105.2 1.0 OE1 P:GLN11 4.8 99.2 1.0

Magnesium binding site 9 out of 12 in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 9 of Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg502 b:79.3 occ:1.00 O3B E:GTP501 2.0 77.9 1.0 O1B E:GTP501 2.0 77.9 1.0 OE2 E:GLU71 2.0 80.2 1.0 O3G E:GTP501 2.2 77.9 1.0 PB E:GTP501 2.4 77.9 1.0 PG E:GTP501 2.4 77.9 1.0 CD E:GLU71 3.0 80.2 1.0 O2G E:GTP501 3.1 77.9 1.0 O2B E:GTP501 3.4 77.9 1.0 OE1 E:GLU71 3.4 80.2 1.0 CB E:ASP98 3.6 82.8 1.0 O3A E:GTP501 3.6 77.9 1.0 OD2 E:ASP98 3.7 82.8 1.0 CG E:ASP98 3.8 82.8 1.0 O1G E:GTP501 3.8 77.9 1.0 CG E:GLU71 4.2 80.2 1.0 CG2 E:THR145 4.4 71.3 1.0 NZ R:LYS252 4.5 78.2 1.0 OD1 E:ASP98 4.6 82.8 1.0 OD1 E:ASP69 4.9 75.6 1.0 CA E:ASP98 5.0 82.8 1.0

Magnesium binding site 10 out of 12 in the Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 10 of Undecorated 13PF Mosaic 20%E254Q - 80% E254QN Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg502 b:72.3 occ:1.00 O3B F:GTP501 2.0 72.6 1.0 O1B F:GTP501 2.1 72.6 1.0 PB F:GTP501 2.4 72.6 1.0 OE2 F:GLU69 3.0 74.3 1.0 PG F:GTP501 3.2 72.6 1.0 O2B F:GTP501 3.2 72.6 1.0 O3G F:GTP501 3.5 72.6 1.0 O2G F:GTP501 3.7 72.6 1.0 O3A F:GTP501 3.8 72.6 1.0 CD F:GLU69 4.3 74.3 1.0 O1G F:GTP501 4.4 72.6 1.0 CG2 F:THR143 4.5 68.7 1.0 OE1 F:GLN11 4.5 61.9 1.0 CB F:GLU69 4.7 74.3 1.0 OD2 F:ASP67 4.7 71.5 1.0 OD1 F:ASP67 4.8 71.5 1.0 CB F:GLN11 4.8 61.9 1.0

J.Estevez-Gallego, T.B.Blum, F.Ruhnow, M.Gili, S.Speroni, R.Garcia-Castellanos, M.O.Steinmetz, T.Surrey. Hydrolysis-Deficient Mosaic Microtubules As Faithful Mimics of the Gtp Cap. Nat Commun V. 16 2396 2025.
ISSN: ESSN 2041-1723
PubMed: 40064882
DOI: 10.1038/S41467-025-57555-6