Magnesium in PDB 9g0a: Influenza A/H7N9 Polymerase Post-Cleavage Cap-Snatching Complex

All present enzymatic activity of Influenza A/H7N9 Polymerase Post-Cleavage Cap-Snatching Complex:
2.7.7.48; 2.7.7.6;

The structure of Influenza A/H7N9 Polymerase Post-Cleavage Cap-Snatching Complex also contains other interesting chemical elements:

Zinc

(Zn)

8 atoms

The binding sites of Magnesium atom in the Influenza A/H7N9 Polymerase Post-Cleavage Cap-Snatching Complex (pdb code 9g0a). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Influenza A/H7N9 Polymerase Post-Cleavage Cap-Snatching Complex, PDB code: 9g0a:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Influenza A/H7N9 Polymerase Post-Cleavage Cap-Snatching Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Influenza A/H7N9 Polymerase Post-Cleavage Cap-Snatching Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg3103 b:87.2 occ:1.00 O3' P:C35 1.8 32.4 1.0 OD1 A:ASP495 1.8 84.6 1.0 OD1 A:ASP499 1.8 92.9 1.0 OD1 A:ASP497 2.3 81.8 1.0 CG A:ASP499 2.6 84.9 1.0 OD2 A:ASP499 2.8 83.5 1.0 O2' P:C35 2.8 45.7 1.0 C3' P:C35 2.8 46.0 1.0 CG A:ASP495 2.8 83.6 1.0 C4' P:C35 3.1 42.8 1.0 OD2 A:ASP495 3.2 85.6 1.0 CG A:ASP497 3.3 77.4 1.0 C2' P:C35 3.3 44.6 1.0 OD2 A:ASP497 3.6 80.5 1.0 O A:ASP495 3.8 78.4 1.0 CB A:ASP499 4.0 72.5 1.0 O4' P:C35 4.1 19.0 1.0 CB A:ASP495 4.1 74.7 1.0 C5' P:C35 4.2 53.2 1.0 C1' P:C35 4.2 33.0 1.0 N A:ASP495 4.2 74.8 1.0 NH2 A:ARG460 4.3 77.2 1.0 C A:ASP495 4.3 73.1 1.0 CA A:ASP495 4.4 70.0 1.0 CA A:ASP499 4.5 68.7 1.0 N A:ASP499 4.6 65.9 1.0 N A:ASP497 4.6 67.1 1.0 CB A:ASP497 4.7 66.7 1.0 O A:ASP497 4.9 77.5 1.0 C A:ASP497 4.9 68.4 1.0 CA A:ASP497 5.0 62.1 1.0

Magnesium binding site 2 out of 2 in the Influenza A/H7N9 Polymerase Post-Cleavage Cap-Snatching Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Influenza A/H7N9 Polymerase Post-Cleavage Cap-Snatching Complex within 5.0Å range: probe atom residue distance (Å) B Occ a:Mg1001 b:105.5 occ:1.00 O a:ILE120 2.4 105.5 1.0 OD1 a:ASP119 2.4 95.9 1.0 OP1 P:A12 2.4 134.5 1.0 P P:A12 2.5 139.8 1.0 OD2 a:ASP108 2.7 88.2 1.0 NE2 a:HIS41 2.8 89.7 1.0 CG a:ASP119 3.3 106.8 1.0 O5' P:A12 3.6 133.6 1.0 OD2 a:ASP119 3.6 113.2 1.0 C a:ILE120 3.6 111.1 1.0 CE1 a:HIS41 3.7 97.3 1.0 CD2 a:HIS41 3.8 92.5 1.0 OP2 P:A12 3.8 131.5 1.0 OP2 P:A13 3.8 133.7 1.0 CG a:ASP108 3.8 93.7 1.0 CA a:GLY121 4.2 90.1 1.0 C3' P:A12 4.3 133.0 1.0 N a:ILE120 4.3 117.5 1.0 N a:GLY121 4.3 92.4 1.0 NZ a:LYS134 4.4 91.3 1.0 OD1 a:ASP108 4.4 90.4 1.0 CE a:LYS134 4.4 96.2 1.0 O3' P:A12 4.4 130.9 1.0 P P:A13 4.5 132.4 1.0 CA a:ILE120 4.6 118.7 1.0 C4' P:A12 4.7 132.3 1.0 C a:ASP119 4.7 108.2 1.0 CB a:ASP119 4.7 108.4 1.0 C5' P:A12 4.7 131.7 1.0 ND1 a:HIS41 4.9 96.7 1.0 CG a:HIS41 4.9 96.6 1.0 OP1 P:A13 5.0 127.4 1.0 CB a:ASP108 5.0 89.8 1.0

A.H.Rotsch, D.Li, M.Dupont, T.Krischuns, C.Oberthuer, A.Stelfox, M.Lukarska, I.Fianu, M.Lidschreiber, N.Naffakh, C.Dienemann, S.Cusack, P.Cramer. Mechanism of Co-Transcriptional Cap-Snatching By Influenza Polymerase To Be Published.