Magnesium in PDB 9izp: Cryo-Em Structure of CASLAMBDA2-Crrna-Target Dna Ternary Complex in the Incompetent State

The binding sites of Magnesium atom in the Cryo-Em Structure of CASLAMBDA2-Crrna-Target Dna Ternary Complex in the Incompetent State (pdb code 9izp). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Cryo-Em Structure of CASLAMBDA2-Crrna-Target Dna Ternary Complex in the Incompetent State, PDB code: 9izp:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Cryo-Em Structure of CASLAMBDA2-Crrna-Target Dna Ternary Complex in the Incompetent State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of CASLAMBDA2-Crrna-Target Dna Ternary Complex in the Incompetent State within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg801 b:26.2 occ:1.00 OP1 B:G-30 2.0 32.8 1.0 OD1 A:ASP499 2.1 33.0 1.0 CG A:ASP499 3.2 33.3 1.0 P B:G-30 3.3 28.7 1.0 OD2 A:ASP499 3.5 35.8 1.0 OP2 B:G-30 3.9 23.4 1.0 O5' B:G-30 4.0 30.8 1.0 NE2 A:GLN498 4.3 33.8 1.0 C5' B:G-30 4.4 23.6 1.0 CB A:ASP499 4.5 25.7 1.0 OP2 B:U-29 4.5 26.1 1.0 O3' B:U-31 4.5 31.3 1.0 O A:ASN495 4.5 34.2 1.0 CA A:ASP499 4.9 24.9 1.0 N A:ASP499 4.9 27.1 1.0 CD A:GLN498 4.9 26.2 1.0 CG A:GLN498 5.0 24.0 1.0

Magnesium binding site 2 out of 3 in the Cryo-Em Structure of CASLAMBDA2-Crrna-Target Dna Ternary Complex in the Incompetent State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of CASLAMBDA2-Crrna-Target Dna Ternary Complex in the Incompetent State within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg101 b:26.5 occ:1.00 O4 B:U-29 3.2 23.7 1.0 O6 B:G-30 3.2 18.1 1.0 O4 B:U-31 3.5 12.9 1.0 N7 B:G-30 3.9 13.0 1.0 C4 B:U-31 4.0 12.3 1.0 N6 B:A-5 4.0 24.0 1.0 C6 B:G-30 4.1 13.3 1.0 C4 B:U-29 4.3 18.9 1.0 C5 B:G-30 4.3 11.2 1.0 C5 B:U-31 4.4 13.8 1.0 N3 B:U-31 4.8 9.2 1.0 N6 B:A-3 4.8 12.6 1.0 N4 B:C-4 4.9 23.6 1.0 C5 B:U-29 5.0 15.1 1.0

Magnesium binding site 3 out of 3 in the Cryo-Em Structure of CASLAMBDA2-Crrna-Target Dna Ternary Complex in the Incompetent State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Cryo-Em Structure of CASLAMBDA2-Crrna-Target Dna Ternary Complex in the Incompetent State within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg102 b:48.8 occ:1.00 O4 B:U-23 2.1 61.4 1.0 C4 B:U-23 3.1 57.2 1.0 C5 B:U-23 3.7 60.5 1.0 N3 B:U-23 4.3 55.3 1.0 N7 B:A-24 4.7 62.5 1.0 C6 B:U-23 5.0 64.8 1.0

S.N.Omura, H.Hirano, Y.Itoh, O.Nureki. Structural Basis For the Target Dna Cleavage and Guide Rna Processing By the Crispr-CASLAMBDA2 To Be Published.