Magnesium in PDB 9kln: Cryo-Em Structure of CHCAS12B-Sgrna-Target Dna Ternary Complex (Complex-A)

The binding sites of Magnesium atom in the Cryo-Em Structure of CHCAS12B-Sgrna-Target Dna Ternary Complex (Complex-A) (pdb code 9kln). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cryo-Em Structure of CHCAS12B-Sgrna-Target Dna Ternary Complex (Complex-A), PDB code: 9kln:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cryo-Em Structure of CHCAS12B-Sgrna-Target Dna Ternary Complex (Complex-A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of CHCAS12B-Sgrna-Target Dna Ternary Complex (Complex-A) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg201 b:44.5 occ:1.00 O B:HOH308 2.1 47.2 1.0 OP2 B:U33 2.1 50.9 1.0 O B:HOH307 2.1 51.4 1.0 O B:HOH305 2.1 51.0 1.0 O B:HOH306 2.1 45.5 1.0 O B:HOH301 2.2 45.6 1.0 P B:U33 3.6 48.5 1.0 N7 B:G56 4.1 42.1 1.0 O5' B:G32 4.2 49.0 1.0 OP1 B:G32 4.3 47.5 1.0 O5' B:U33 4.3 49.6 1.0 O6 B:G56 4.3 46.4 1.0 OP1 B:U33 4.3 50.4 1.0 OP2 B:G55 4.5 54.9 1.0 O5' B:G55 4.5 52.0 1.0 O3' B:G32 4.6 42.8 1.0 C2' B:G55 4.7 46.1 1.0 P B:G32 4.7 48.7 1.0 O3' B:C31 4.8 49.7 1.0 C5 B:G56 4.9 41.8 1.0 C6 B:G56 4.9 42.6 1.0 C3' B:G55 5.0 47.3 1.0 C3' B:G32 5.0 42.4 1.0

Magnesium binding site 2 out of 2 in the Cryo-Em Structure of CHCAS12B-Sgrna-Target Dna Ternary Complex (Complex-A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of CHCAS12B-Sgrna-Target Dna Ternary Complex (Complex-A) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg202 b:50.2 occ:1.00 O B:HOH302 2.0 51.7 1.0 OP2 B:C39 2.0 49.0 1.0 O B:HOH304 2.1 52.2 1.0 OP2 B:U38 2.1 50.8 1.0 O B:HOH303 2.1 49.6 1.0 P B:U38 3.3 49.3 1.0 P B:C39 3.5 50.0 1.0 O5' B:U38 3.5 47.3 1.0 OP1 B:U35 3.8 54.7 1.0 OP1 B:C39 4.2 52.4 1.0 OP2 B:C36 4.2 46.3 1.0 O5' B:C39 4.2 46.3 1.0 OP1 B:U38 4.2 50.5 1.0 OP1 B:C36 4.4 50.5 1.0 O3' B:U37 4.5 46.3 1.0 O3' B:U38 4.5 51.3 1.0 C6 B:C39 4.6 44.3 1.0 C5 B:C39 4.6 42.6 1.0 C5' B:U38 4.7 45.1 1.0 P B:C36 4.8 49.2 1.0 C3' B:U38 4.8 45.2 1.0 P B:U35 4.9 52.8 1.0

Y.Li, J.Li, X.Pei, J.Wei, J.Gan, J.Lin. Catalytic-State Structure of Candidatus Hydrogenedentes CAS12B Revealed By Cryo-Em Studies. Nucleic Acids Res. V. 53 2025.
ISSN: ESSN 1362-4962
PubMed: 40539514
DOI: 10.1093/NAR/GKAF519