Atomistry » Magnesium » PDB 1fp6-1g7t » 1fsg
Atomistry »
  Magnesium »
    PDB 1fp6-1g7t »
      1fsg »

Magnesium in PDB 1fsg: Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions

Enzymatic activity of Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions

All present enzymatic activity of Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions:
2.4.2.8;

Protein crystallography data

The structure of Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions, PDB code: 1fsg was solved by A.Heroux, E.L.White, L.J.Ross, A.P.Kuzin, D.W.Borhani, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 35.00 / 1.05
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 69.330, 90.180, 75.720, 90.00, 90.00, 90.00
R / Rfree (%) 12.2 / 15.4

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions (pdb code 1fsg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions, PDB code: 1fsg:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in 1fsg

Go back to Magnesium Binding Sites List in 1fsg
Magnesium binding site 1 out of 4 in the Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg301

b:6.4
occ:1.00
 O1B A:PRP303 2.0 6.4 1.0
O A:HOH1931 2.0 7.4 1.0
O A:HOH1930 2.1 6.5 1.0
O2 A:PRP303 2.1 6.5 1.0
O3 A:PRP303 2.1 6.6 1.0
O1 A:PRP303 2.3 6.5 1.0
C2 A:PRP303 2.8 6.7 1.0
C3 A:PRP303 3.0 6.3 1.0
C1 A:PRP303 3.1 6.5 1.0
PB A:PRP303 3.2 6.5 1.0
O3A A:PRP303 3.3 6.6 1.0
PA A:PRP303 3.5 6.9 1.0
OE1 A:GLU146 4.0 6.5 0.6
O4 A:PRP303 4.0 6.9 1.0
OD2 A:ASP147 4.0 7.6 1.0
O2B A:PRP303 4.0 6.7 1.0
N A:GLY80 4.0 6.3 1.0
O A:HOH1009 4.0 8.1 1.0
C4 A:PRP303 4.1 6.8 1.0
OE1 A:GLU146 4.1 6.8 0.4
OE2 A:GLU146 4.1 9.3 0.6
OE2 A:GLU146 4.2 6.2 0.4
O3B A:PRP303 4.3 7.2 1.0
CA A:GLY80 4.4 6.7 1.0
O1A A:PRP303 4.4 7.5 1.0
O A:LEU78 4.4 7.1 1.0
CD A:GLU146 4.5 9.5 0.6
O A:LEU115 4.5 7.8 1.0
O2A A:PRP303 4.5 7.1 1.0
CG2 A:ILE77 4.5 7.8 1.0
CD A:GLU146 4.6 6.6 0.4
C A:LEU78 4.7 6.4 1.0
N A:LYS79 4.7 6.2 1.0
O A:ILE77 4.7 6.9 1.0
CG A:ASP147 4.8 6.9 1.0
O A:HOH1934 4.8 7.2 1.0
O A:HOH1007 4.9 7.5 1.0
OD1 A:ASP147 4.9 8.8 1.0

Magnesium binding site 2 out of 4 in 1fsg

Go back to Magnesium Binding Sites List in 1fsg
Magnesium binding site 2 out of 4 in the Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg302

b:6.7
occ:1.00
 O2A A:PRP303 2.0 7.1 1.0
O A:HOH1934 2.0 7.2 1.0
O2B A:PRP303 2.1 6.7 1.0
OD1 A:ASP206 2.1 6.5 1.0
O A:HOH1933 2.1 8.1 1.0
O A:HOH1932 2.1 7.7 1.0
CG A:ASP206 3.1 6.8 1.0
PA A:PRP303 3.2 6.9 1.0
PB A:PRP303 3.3 6.5 1.0
OD2 A:ASP206 3.4 7.5 1.0
O3A A:PRP303 3.5 6.6 1.0
O1 A:PRP303 3.9 6.5 1.0
O A:HOH1007 3.9 7.5 1.0
NH2 A:ARG212 4.0 6.9 1.0
C1 A:PRP303 4.2 6.5 1.0
O A:ASP206 4.3 7.8 1.0
O1B A:PRP303 4.3 6.4 1.0
O3B A:PRP303 4.3 7.2 1.0
NH1 A:ARG212 4.4 6.8 1.0
O A:HOH1012 4.4 9.3 1.0
CB A:ASP206 4.5 6.9 1.0
O1A A:PRP303 4.5 7.5 1.0
N3 A:9DG304 4.5 7.1 1.0
O A:HOH1029 4.5 11.3 1.0
O A:HOH1045 4.6 18.1 1.0
CZ A:ARG212 4.6 6.6 1.0
N A:ASP206 4.7 6.2 1.0
O2 A:PRP303 4.7 6.5 1.0
C A:ASP206 4.8 6.9 1.0
CA A:ASP206 4.9 6.4 1.0
CB A:TYR118 4.9 9.0 1.0

Magnesium binding site 3 out of 4 in 1fsg

Go back to Magnesium Binding Sites List in 1fsg
Magnesium binding site 3 out of 4 in the Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg301

b:8.0
occ:1.00
 O1B C:PRP303 2.0 7.9 1.0
O C:HOH1935 2.0 8.0 1.0
O C:HOH1936 2.0 8.9 1.0
O2 C:PRP303 2.1 8.5 1.0
O3 C:PRP303 2.1 8.3 1.0
O1 C:PRP303 2.3 8.2 1.0
C2 C:PRP303 2.8 8.3 1.0
C3 C:PRP303 3.0 8.1 1.0
C1 C:PRP303 3.1 8.5 1.0
PB C:PRP303 3.2 8.0 1.0
O3A C:PRP303 3.3 8.5 1.0
PA C:PRP303 3.5 8.4 1.0
OE1 C:GLU146 3.9 9.0 0.5
O4 C:PRP303 4.0 8.6 1.0
O2B C:PRP303 4.0 8.2 1.0
OE1 C:GLU146 4.0 8.2 0.5
OD2 C:ASP147 4.0 9.0 1.0
N C:GLY80 4.0 7.6 1.0
O C:HOH1075 4.0 9.8 1.0
C4 C:PRP303 4.1 8.4 1.0
OE2 C:GLU146 4.1 8.4 0.5
OE2 C:GLU146 4.2 10.1 0.5
CA C:GLY80 4.3 7.8 1.0
O3B C:PRP303 4.3 8.6 1.0
O1A C:PRP303 4.4 9.0 1.0
O C:LEU78 4.4 8.7 1.0
CD C:GLU146 4.5 9.5 0.5
CD C:GLU146 4.5 11.3 0.5
O C:LEU115 4.5 9.3 1.0
CG2 C:ILE77 4.5 9.3 1.0
O2A C:PRP303 4.5 8.6 1.0
O C:ILE77 4.7 8.5 1.0
N C:LYS79 4.7 7.8 1.0
C C:LEU78 4.7 7.2 1.0
CG C:ASP147 4.9 8.4 1.0
O C:HOH1939 4.9 9.1 1.0
O C:HOH1073 4.9 9.4 1.0
OD1 C:ASP147 5.0 9.9 1.0

Magnesium binding site 4 out of 4 in 1fsg

Go back to Magnesium Binding Sites List in 1fsg
Magnesium binding site 4 out of 4 in the Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Toxoplasma Gondii Hypoxanthine-Guanine Phosphoribosyltransferase Complexed with 9-Deazaguanine, Alpha-D-5-Phosphoribosyl-1- Pyrophosphate (Prpp) and Two MG2+ Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg302

b:8.5
occ:1.00
 O2A C:PRP303 2.0 8.6 1.0
O2B C:PRP303 2.0 8.2 1.0
O C:HOH1939 2.1 9.1 1.0
OD1 C:ASP206 2.1 8.2 1.0
O C:HOH1938 2.1 9.2 1.0
O C:HOH1937 2.1 9.0 1.0
CG C:ASP206 3.1 8.2 1.0
PA C:PRP303 3.2 8.4 1.0
PB C:PRP303 3.3 8.0 1.0
OD2 C:ASP206 3.4 9.2 1.0
O3A C:PRP303 3.5 8.5 1.0
O1 C:PRP303 3.9 8.2 1.0
O C:HOH1073 3.9 9.4 1.0
NH2 C:ARG212 4.0 8.3 1.0
C1 C:PRP303 4.2 8.5 1.0
O C:ASP206 4.3 9.4 1.0
O1B C:PRP303 4.3 7.9 1.0
O3B C:PRP303 4.4 8.6 1.0
NH1 C:ARG212 4.4 8.4 1.0
O C:HOH1078 4.4 10.6 1.0
CB C:ASP206 4.4 8.0 1.0
N3 C:9DG304 4.5 9.0 1.0
O1A C:PRP303 4.5 9.0 1.0
O C:HOH1095 4.5 11.6 1.0
O C:HOH1109 4.5 19.7 1.0
CZ C:ARG212 4.7 8.3 1.0
N C:ASP206 4.7 8.1 1.0
O2 C:PRP303 4.7 8.5 1.0
C C:ASP206 4.8 8.2 1.0
CA C:ASP206 4.9 8.0 1.0
CB C:TYR118 4.9 10.9 1.0
C2 C:PRP303 5.0 8.3 1.0

Reference:

A.Heroux, E.L.White, L.J.Ross, A.P.Kuzin, D.W.Borhani. Substrate Deformation in A Hypoxanthine-Guanine Phosphoribosyltransferase Ternary Complex: the Structural Basis For Catalysis. Structure Fold.Des. V. 8 1309 2000.
ISSN: ISSN 0969-2126
PubMed: 11188695
DOI: 10.1016/S0969-2126(00)00546-3
Page generated: Tue Aug 13 03:35:07 2024

Last articles

Cl in 3QP0
Cl in 3QMN
Cl in 3QOC
Cl in 3QNM
Cl in 3QNK
Cl in 3QNG
Cl in 3QN8
Cl in 3QLJ
Cl in 3QM3
Cl in 3QLU
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy