Magnesium in PDB 1ii6: Crystal Structure of the Mitotic Kinesin EG5 in Complex with Mg-Adp.

The structure of Crystal Structure of the Mitotic Kinesin EG5 in Complex with Mg-Adp., PDB code: 1ii6 was solved by J.Turner, R.Anderson, J.Guo, C.Beraud, R.Sakowicz, R.Fletterick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	53.083, 78.588, 94.147, 90.00, 93.84, 90.00
R / Rfree (%)	21.8 / 25.5

The binding sites of Magnesium atom in the Crystal Structure of the Mitotic Kinesin EG5 in Complex with Mg-Adp. (pdb code 1ii6). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Mitotic Kinesin EG5 in Complex with Mg-Adp., PDB code: 1ii6:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Mitotic Kinesin EG5 in Complex with Mg-Adp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Mitotic Kinesin EG5 in Complex with Mg-Adp. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg2603 b:29.8 occ:1.00 OG1 A:THR112 2.3 21.9 1.0 O A:HOH2613 2.3 18.4 1.0 O A:HOH2612 2.4 24.1 1.0 O A:HOH2614 2.4 20.3 1.0 O A:HOH2726 2.4 23.0 1.0 O3B A:ADP2601 2.6 32.2 1.0 CB A:THR112 3.3 19.8 1.0 PB A:ADP2601 3.3 26.2 1.0 O1B A:ADP2601 3.4 27.7 1.0 O3A A:ADP2601 3.6 32.9 1.0 N A:THR112 3.8 20.6 1.0 OD2 A:ASP265 4.0 22.0 1.0 O A:HOH2627 4.1 31.1 1.0 O A:HOH2686 4.1 20.9 1.0 CA A:THR112 4.2 21.0 1.0 OD1 A:ASP265 4.3 23.0 1.0 O1A A:ADP2601 4.4 30.4 1.0 CG2 A:THR112 4.5 21.6 1.0 O2A A:ADP2601 4.5 29.1 1.0 O A:HOH2611 4.5 17.7 1.0 CG A:ASP265 4.6 25.2 1.0 O A:HOH2664 4.6 35.3 1.0 CB A:LYS111 4.6 15.9 1.0 PA A:ADP2601 4.7 27.9 1.0 O2B A:ADP2601 4.8 36.0 1.0 C A:LYS111 4.9 21.3 1.0 CE A:LYS111 4.9 21.1 1.0 O A:HOH2615 4.9 21.7 1.0 NZ A:LYS111 4.9 20.3 1.0 OG A:SER232 5.0 35.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Mitotic Kinesin EG5 in Complex with Mg-Adp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Mitotic Kinesin EG5 in Complex with Mg-Adp. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2602 b:25.9 occ:1.00 OG1 B:THR112 2.3 15.1 1.0 O B:HOH2609 2.3 20.7 1.0 O B:HOH2605 2.4 14.0 1.0 O B:HOH2606 2.4 19.5 1.0 O B:HOH2607 2.4 14.3 1.0 O3B B:ADP2600 2.4 12.1 1.0 O2B B:ADP2600 3.3 19.4 1.0 CB B:THR112 3.3 16.5 1.0 O1A B:ADP2600 3.6 27.7 1.0 PB B:ADP2600 3.8 22.1 1.0 O B:HOH2749 3.9 25.6 1.0 N B:THR112 4.0 15.7 1.0 O3A B:ADP2600 4.0 24.9 1.0 OD2 B:ASP265 4.1 14.3 1.0 CA B:THR112 4.2 14.9 1.0 O B:HOH2612 4.2 14.4 1.0 OD1 B:ASP265 4.3 15.5 1.0 O1B B:ADP2600 4.3 16.0 1.0 O B:HOH2611 4.3 16.4 1.0 CG2 B:THR112 4.4 15.9 1.0 O B:HOH2683 4.4 28.9 1.0 PA B:ADP2600 4.5 24.6 1.0 O A:HOH2608 4.5 25.4 1.0 CG B:ASP265 4.7 14.8 1.0 CB B:LYS111 4.8 15.3 1.0 CE B:LYS111 4.9 13.8 1.0 O B:HOH2613 5.0 17.5 1.0

J.Turner, R.Anderson, J.Guo, C.Beraud, R.Fletterick, R.Sakowicz. Crystal Structure of the Mitotic Spindle Kinesin EG5 Reveals A Novel Conformation of the Neck-Linker. J.Biol.Chem. V. 276 25496 2001.
ISSN: ISSN 0021-9258
PubMed: 11328809
DOI: 10.1074/JBC.M100395200