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Magnesium in PDB 1sl0: Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide
Enzymatic activity of Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide
All present enzymatic activity of Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide:
2.7.7.7;
2.7.7.7;
Protein crystallography data
The structure of Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide, PDB code: 1sl0
was solved by
Y.Li,
S.Dutta,
S.Doublie,
H.M.Bdour,
J.S.Taylor,
T.Ellenberger,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
| Resolution Low / High (Å) | 50.00 / 3.20 |
| Space group | P 1 21 1 |
| Cell size a, b, c (Å), α, β, γ (°) | 54.266, 105.466, 213.931, 90.00, 91.57, 90.00 |
| R / Rfree (%) | 28.2 / 35.1 |
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide
(pdb code 1sl0). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide, PDB code: 1sl0:
Jump to Magnesium binding site number: 1; 2;
In total 2 binding sites of Magnesium where determined in the Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide, PDB code: 1sl0:
Jump to Magnesium binding site number: 1; 2;
Magnesium binding site 1 out of 2 in 1sl0
Go back to
Magnesium Binding Sites List in 1sl0
Magnesium binding site 1 out
of 2 in the Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide within 5.0Å range:
|
Magnesium binding site 2 out of 2 in 1sl0
Go back to
Magnesium Binding Sites List in 1sl0
Magnesium binding site 2 out
of 2 in the Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Ternary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Disordered Cis-Syn Thymine Dimer on the Template and An Incoming Nucleotide within 5.0Å range:
|
Reference:
Y.Li,
S.Dutta,
S.Doublie,
H.M.Bdour,
J.S.Taylor,
T.Ellenberger.
Nucleotide Insertion Opposite A Cis-Syn Thymine Dimer By A Replicative Dna Polymerase From Bacteriophage T7. Nat.Struct.Mol.Biol. V. 11 784 2004.
ISSN: ISSN 1545-9993
PubMed: 15235589
DOI: 10.1038/NSMB792
Page generated: Tue Aug 13 13:46:18 2024
ISSN: ISSN 1545-9993
PubMed: 15235589
DOI: 10.1038/NSMB792
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