Magnesium in PDB 1umx: Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L)

The structure of Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L), PDB code: 1umx was solved by P.K.Fyfe, N.W.Isaacs, R.J.Cogdell, M.R.Jones, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.00 / 2.80
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	141.638, 141.638, 187.394, 90.00, 90.00, 120.00
R / Rfree (%)	22.4 / 24.9

The structure of Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L) also contains other interesting chemical elements:

Iron

(Fe)

1 atom

The binding sites of Magnesium atom in the Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L) (pdb code 1umx). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L), PDB code: 1umx:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L) within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg1282 b:33.6 occ:1.00 MG L:BCL1282 0.0 33.6 1.0 NB L:BCL1282 2.1 32.0 1.0 ND L:BCL1282 2.1 35.7 1.0 NC L:BCL1282 2.1 33.0 1.0 NA L:BCL1282 2.2 33.1 1.0 NE2 L:HIS173 2.3 26.8 1.0 C4B L:BCL1282 3.0 31.8 1.0 C1B L:BCL1282 3.1 31.0 1.0 C4D L:BCL1282 3.1 35.5 1.0 C1C L:BCL1282 3.1 32.3 1.0 C4A L:BCL1282 3.1 30.2 1.0 C1D L:BCL1282 3.2 32.8 1.0 C4C L:BCL1282 3.2 31.2 1.0 C1A L:BCL1282 3.3 34.9 1.0 CD2 L:HIS173 3.3 24.1 1.0 CE1 L:HIS173 3.3 22.9 1.0 CHB L:BCL1282 3.4 29.4 1.0 CHC L:BCL1282 3.4 31.3 1.0 CHD L:BCL1282 3.5 29.5 1.0 CHA L:BCL1282 3.6 34.2 1.0 CBB M:BCL1304 3.8 32.6 1.0 CAB M:BCL1304 4.0 36.7 1.0 C3B L:BCL1282 4.2 31.3 1.0 C2B L:BCL1282 4.2 31.0 1.0 C3D L:BCL1282 4.3 31.4 1.0 OBB M:BCL1304 4.3 45.6 1.0 C2D L:BCL1282 4.4 35.6 1.0 ND1 L:HIS173 4.4 26.9 1.0 CG L:HIS173 4.4 28.5 1.0 C3A L:BCL1282 4.4 34.8 1.0 C2C L:BCL1282 4.5 28.2 1.0 C3C L:BCL1282 4.5 27.4 1.0 C2A L:BCL1282 4.6 34.8 1.0 C3B M:BCL1304 4.7 33.7 1.0 CD2 L:PHE167 4.7 31.8 1.0 CMA L:BCL1282 4.9 28.5 1.0

Magnesium binding site 2 out of 4 in the Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L) within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg1283 b:14.3 occ:1.00 MG L:BCL1283 0.0 14.3 1.0 NC L:BCL1283 1.9 8.7 1.0 ND L:BCL1283 2.1 5.3 1.0 NA L:BCL1283 2.1 12.6 1.0 NB L:BCL1283 2.1 10.4 1.0 NE2 L:HIS153 2.5 37.9 1.0 C1C L:BCL1283 2.9 15.0 1.0 C4D L:BCL1283 2.9 7.7 1.0 C4C L:BCL1283 3.0 11.2 1.0 C4B L:BCL1283 3.1 15.0 1.0 C1A L:BCL1283 3.1 17.3 1.0 C4A L:BCL1283 3.1 14.2 1.0 C1B L:BCL1283 3.1 17.8 1.0 C1D L:BCL1283 3.2 11.1 1.0 CHC L:BCL1283 3.3 11.7 1.0 CHA L:BCL1283 3.3 13.5 1.0 CE1 L:HIS153 3.4 34.9 1.0 CD2 L:HIS153 3.5 37.9 1.0 CHD L:BCL1283 3.5 8.2 1.0 CHB L:BCL1283 3.6 11.1 1.0 C2C L:BCL1283 4.2 17.9 1.0 C3C L:BCL1283 4.2 17.2 1.0 C3D L:BCL1283 4.3 9.5 1.0 C3B L:BCL1283 4.3 18.7 1.0 C2B L:BCL1283 4.3 17.6 1.0 C2D L:BCL1283 4.4 10.9 1.0 C3A L:BCL1283 4.4 16.1 1.0 C2A L:BCL1283 4.4 19.0 1.0 ND1 L:HIS153 4.6 39.9 1.0 CG L:HIS153 4.6 41.2 1.0 CE2 M:TYR210 4.6 34.3 1.0 CBC L:BCL1283 4.6 20.4 1.0 CBD L:BCL1283 4.8 20.3 1.0 CMC L:BCL1283 4.9 20.6 1.0 OBB L:BPB1284 4.9 45.5 1.0 CAC L:BCL1283 5.0 21.7 1.0

Magnesium binding site 3 out of 4 in the Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L) within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg1303 b:33.1 occ:1.00 MG M:BCL1303 0.0 33.1 1.0 NB M:BCL1303 2.0 36.9 1.0 ND M:BCL1303 2.0 31.6 1.0 NC M:BCL1303 2.1 33.9 1.0 NE2 M:HIS182 2.1 42.0 1.0 NA M:BCL1303 2.2 33.2 1.0 CD2 M:HIS182 2.9 43.1 1.0 C4D M:BCL1303 2.9 32.1 1.0 C1B M:BCL1303 3.0 38.4 1.0 C4B M:BCL1303 3.0 35.4 1.0 C1C M:BCL1303 3.1 34.6 1.0 C1D M:BCL1303 3.1 31.3 1.0 C4C M:BCL1303 3.1 33.2 1.0 C4A M:BCL1303 3.1 34.8 1.0 C1A M:BCL1303 3.2 33.2 1.0 CE1 M:HIS182 3.2 40.4 1.0 CHC M:BCL1303 3.4 35.2 1.0 CHD M:BCL1303 3.4 31.6 1.0 CHA M:BCL1303 3.4 35.2 1.0 CHB M:BCL1303 3.5 34.1 1.0 C3D M:BCL1303 4.1 33.1 1.0 CG M:HIS182 4.1 40.8 1.0 C3B M:BCL1303 4.2 38.1 1.0 ND1 M:HIS182 4.2 39.9 1.0 C2B M:BCL1303 4.2 38.8 1.0 C2D M:BCL1303 4.2 34.6 1.0 C3C M:BCL1303 4.4 31.3 1.0 C2C M:BCL1303 4.4 30.6 1.0 C3A M:BCL1303 4.5 34.4 1.0 C2A M:BCL1303 4.5 36.0 1.0 CBD M:BCL1303 4.9 36.5 1.0 CM5 M:SPN1308 4.9 48.4 1.0

Magnesium binding site 4 out of 4 in the Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Photosynthetic Reaction Center Mutant with Arg M267 Replaced with Leu (Chain M, R267L) within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg1304 b:31.6 occ:1.00 MG M:BCL1304 0.0 31.6 1.0 NB M:BCL1304 2.0 30.2 1.0 NC M:BCL1304 2.0 31.0 1.0 ND M:BCL1304 2.1 31.8 1.0 NA M:BCL1304 2.2 31.1 1.0 NE2 M:HIS202 2.3 35.1 1.0 C4D M:BCL1304 3.0 33.4 1.0 C1C M:BCL1304 3.0 32.9 1.0 C4B M:BCL1304 3.0 29.5 1.0 C1B M:BCL1304 3.0 30.5 1.0 C4C M:BCL1304 3.1 30.8 1.0 C1D M:BCL1304 3.2 31.0 1.0 C4A M:BCL1304 3.2 30.3 1.0 CE1 M:HIS202 3.2 31.9 1.0 C1A M:BCL1304 3.3 29.3 1.0 CHC M:BCL1304 3.3 27.8 1.0 CBB L:BCL1282 3.4 35.6 1.0 CD2 M:HIS202 3.4 36.0 1.0 CHB M:BCL1304 3.5 26.2 1.0 CHA M:BCL1304 3.5 31.9 1.0 CHD M:BCL1304 3.5 30.8 1.0 CAB L:BCL1282 3.8 31.0 1.0 OBB L:BCL1282 4.1 31.0 1.0 C3D M:BCL1304 4.2 32.1 1.0 C3B M:BCL1304 4.2 33.7 1.0 C2B M:BCL1304 4.3 32.2 1.0 C2D M:BCL1304 4.3 32.4 1.0 C2C M:BCL1304 4.3 32.0 1.0 C3C M:BCL1304 4.3 31.7 1.0 ND1 M:HIS202 4.4 33.8 1.0 CG M:HIS202 4.5 32.3 1.0 C3A M:BCL1304 4.5 32.4 1.0 C2A M:BCL1304 4.6 31.1 1.0 C3B L:BCL1282 4.6 31.3 1.0 CD1 M:ILE206 4.9 34.1 1.0 CBD M:BCL1304 4.9 30.7 1.0

P.K.Fyfe, N.W.Isaacs, R.J.Cogdell, M.R.Jones. Disruption of A Specific Molecular Interaction with A Bound Lipid Affects the Thermal Stability of the Purple Bacterial Reaction Centre. Biochim.Biophys.Acta V.1608 11 2004.
ISSN: ISSN 0006-3002
PubMed: 14741581
DOI: 10.1016/J.BBABIO.2003.09.014