Atomistry » Magnesium » PDB 1ueu-1v5f » 1v14
Atomistry »
  Magnesium »
    PDB 1ueu-1v5f »
      1v14 »

Magnesium in PDB 1v14: Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A)

Enzymatic activity of Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A)

All present enzymatic activity of Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A):
3.1.21.1;

Protein crystallography data

The structure of Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A), PDB code: 1v14 was solved by M.J.Mate, C.Kleanthous, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 74.54 / 2.9
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 92.946, 124.442, 111.227, 90.00, 90.00, 90.00
R / Rfree (%) 21.5 / 30.6

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A) (pdb code 1v14). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A), PDB code: 1v14:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6;

Magnesium binding site 1 out of 6 in 1v14

Go back to Magnesium Binding Sites List in 1v14
Magnesium binding site 1 out of 6 in the Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1134

b:98.4
occ:1.00
 OP1 E:DC6 2.0 58.9 1.0
ND1 A:HIS102 2.9 52.6 1.0
CE1 A:HIS102 3.1 53.4 1.0
P E:DC6 3.3 58.8 1.0
NE2 A:HIS127 3.3 60.7 1.0
C4' E:DT5 3.5 59.8 1.0
O3' E:DT5 3.6 58.4 1.0
C3' E:DT5 3.8 59.7 1.0
C5' E:DT5 4.1 60.2 1.0
O5' E:DC6 4.1 57.5 1.0
ND1 A:HIS131 4.1 63.2 1.0
O5' E:DT5 4.1 61.2 1.0
CD2 A:HIS127 4.1 60.5 1.0
CG A:HIS102 4.2 53.0 1.0
CE1 A:HIS127 4.3 60.5 1.0
NE2 A:HIS102 4.4 53.9 1.0
OP2 E:DC6 4.4 58.7 1.0
CE1 A:HIS131 4.6 63.6 1.0
O A:ALA103 4.7 52.4 1.0
O A:HOH2004 4.7 56.0 1.0
O4' E:DT5 4.7 59.3 1.0
CD2 A:HIS102 5.0 53.2 1.0

Magnesium binding site 2 out of 6 in 1v14

Go back to Magnesium Binding Sites List in 1v14
Magnesium binding site 2 out of 6 in the Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg1134

b:70.5
occ:1.00
 OP1 I:DC6 1.9 60.1 1.0
NE2 C:HIS127 2.2 60.2 1.0
O5' I:DC6 2.3 58.3 1.0
P I:DC6 2.3 58.9 1.0
O3' I:DT5 2.6 59.0 1.0
ND1 C:HIS102 2.6 56.3 1.0
CE1 C:HIS127 3.1 59.9 1.0
CD2 C:HIS127 3.1 59.5 1.0
O C:HOH2006 3.2 44.4 1.0
CE1 C:HIS102 3.3 56.1 1.0
C5' I:DC6 3.7 56.3 1.0
CG C:HIS102 3.7 55.1 1.0
C3' I:DT5 3.8 61.2 1.0
OP2 I:DC6 3.8 57.7 1.0
CA C:HIS102 4.0 54.4 1.0
ND1 C:HIS127 4.1 59.6 1.0
C4' I:DC6 4.2 54.9 1.0
CB C:HIS102 4.2 54.5 1.0
CG C:HIS127 4.2 58.3 1.0
N C:ALA103 4.2 55.1 1.0
C4' I:DT5 4.2 62.3 1.0
O4' I:DC6 4.3 54.7 1.0
NE2 C:HIS102 4.5 55.8 1.0
C C:HIS102 4.6 54.8 1.0
CD2 C:HIS102 4.7 54.9 1.0
O C:HOH2004 4.9 34.3 1.0
O4' I:DT5 4.9 63.0 1.0
C2' I:DT5 5.0 62.0 1.0

Magnesium binding site 3 out of 6 in 1v14

Go back to Magnesium Binding Sites List in 1v14
Magnesium binding site 3 out of 6 in the Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A) within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Mg1009

b:56.1
occ:1.00
 N7 E:DG3 2.9 60.9 1.0
O6 E:DG3 3.7 61.0 1.0
C5 E:DG3 3.8 61.1 1.0
C8 E:DG3 3.8 60.8 1.0
C6 E:DG3 4.1 61.1 1.0
N6 E:DA4 4.7 62.4 1.0

Magnesium binding site 4 out of 6 in 1v14

Go back to Magnesium Binding Sites List in 1v14
Magnesium binding site 4 out of 6 in the Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A) within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Mg1009

b:56.2
occ:1.00
 N7 G:DG3 2.5 56.4 1.0
O6 G:DG3 3.2 56.9 1.0
C5 G:DG3 3.4 56.6 1.0
C8 G:DG3 3.6 56.7 1.0
C6 G:DG3 3.7 57.0 1.0
C4 G:DG3 4.7 56.8 1.0
N6 G:DA4 4.8 56.1 1.0
N9 G:DG3 4.8 56.6 1.0

Magnesium binding site 5 out of 6 in 1v14

Go back to Magnesium Binding Sites List in 1v14
Magnesium binding site 5 out of 6 in the Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A) within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Mg1009

b:76.3
occ:1.00
 N7 I:DG3 2.7 59.0 1.0
O6 I:DG3 3.6 58.4 1.0
C5 I:DG3 3.7 58.7 1.0
C8 I:DG3 3.7 58.9 1.0
C6 I:DG3 4.0 58.9 1.0
C4 I:DG3 4.9 58.7 1.0
N9 I:DG3 4.9 58.9 1.0
N6 I:DA4 4.9 50.5 1.0

Magnesium binding site 6 out of 6 in 1v14

Go back to Magnesium Binding Sites List in 1v14
Magnesium binding site 6 out of 6 in the Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Crystal Structure of the Colicin E9, Mutant HIS103ALA, in Complex with Mg+2 and Dsdna (Resolution 2.9A) within 5.0Å range:
probe atom residue distance (Å) B Occ
K:Mg1009

b:55.2
occ:1.00
 N7 K:DG3 2.7 56.4 1.0
C8 K:DG3 3.6 56.6 1.0
C5 K:DG3 3.7 56.5 1.0
O6 K:DG3 3.8 56.1 1.0
C6 K:DG3 4.1 56.7 1.0
N9 K:DG3 4.8 56.9 1.0
C4 K:DG3 4.9 56.1 1.0

Reference:

M.J.Mate, C.Kleanthous. Structure-Based Analysis of the Metal-Dependent Mechanism of H-N-H Endonucleases J.Biol.Chem. V. 279 34763 2004.
ISSN: ISSN 0021-9258
PubMed: 15190054
DOI: 10.1074/JBC.M403719200
Page generated: Tue Aug 13 14:58:11 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy