Magnesium in PDB 1x9m: T7 Dna Polymerase in Complex with An N-2- Acetylaminofluorene-Adducted Dna

All present enzymatic activity of T7 Dna Polymerase in Complex with An N-2- Acetylaminofluorene-Adducted Dna:
2.7.7.7;

The structure of T7 Dna Polymerase in Complex with An N-2- Acetylaminofluorene-Adducted Dna, PDB code: 1x9m was solved by S.Dutta, Y.Li, D.Johnson, L.Dzantiev, C.C.Richardson, L.J.Romano, T.Ellenberger, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.38 / 2.10
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	105.109, 212.308, 52.070, 90.00, 90.00, 90.00
R / Rfree (%)	21.3 / 23.6

The binding sites of Magnesium atom in the T7 Dna Polymerase in Complex with An N-2- Acetylaminofluorene-Adducted Dna (pdb code 1x9m). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the T7 Dna Polymerase in Complex with An N-2- Acetylaminofluorene-Adducted Dna, PDB code: 1x9m:

Magnesium binding site 1 out of 1 in the T7 Dna Polymerase in Complex with An N-2- Acetylaminofluorene-Adducted Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of T7 Dna Polymerase in Complex with An N-2- Acetylaminofluorene-Adducted Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg3001 b:42.1 occ:1.00 O A:HOH4152 2.5 34.3 1.0 O A:HOH4275 2.5 36.7 1.0 O A:HOH4276 2.5 25.9 1.0 O A:HOH4277 2.6 33.8 1.0 OD1 A:ASP5 2.7 28.9 1.0 O A:HOH4316 3.7 31.9 1.0 CG A:ASP5 3.7 26.1 1.0 OD2 A:ASP174 3.8 47.1 1.0 OD2 A:ASP5 3.9 29.8 1.0 O A:HOH4069 4.2 32.2 1.0 O A:ILE6 4.3 22.5 1.0 OD2 A:ASP65 4.4 23.7 1.0 OD1 A:ASP65 4.5 23.6 1.0 O A:HOH4079 4.6 32.1 1.0 O A:HIS59 4.6 16.1 1.0 CE A:MET107 4.6 35.2 1.0 O A:HOH4058 4.8 23.8 1.0 CG A:ASP65 4.9 23.4 1.0 CG A:ASP174 4.9 39.7 1.0

S.Dutta, Y.Li, D.Johnson, L.Dzantiev, C.C.Richardson, L.J.Romano, T.Ellenberger. Crystal Structures of 2-Acetylaminofluorene and 2-Aminofluorene in Complex with T7 Dna Polymerase Reveal Mechanisms of Mutagenesis. Proc.Natl.Acad.Sci.Usa V. 101 16186 2004.
ISSN: ISSN 0027-8424
PubMed: 15528277
DOI: 10.1073/PNAS.0406516101