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Magnesium in PDB 1x9s: T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Ctp As the Incoming Nucleotide.
Enzymatic activity of T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Ctp As the Incoming Nucleotide.
All present enzymatic activity of T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Ctp As the Incoming Nucleotide.:
2.7.7.7;
2.7.7.7;
Protein crystallography data
The structure of T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Ctp As the Incoming Nucleotide., PDB code: 1x9s
was solved by
S.Dutta,
Y.Li,
D.Johnson,
L.Dzantiev,
C.C.Richardson,
L.J.Romano,
T.Ellenberger,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
| Resolution Low / High (Å) | 46.64 / 2.70 |
| Space group | P 21 21 2 |
| Cell size a, b, c (Å), α, β, γ (°) | 104.579, 211.103, 51.991, 90.00, 90.00, 90.00 |
| R / Rfree (%) | 22.6 / 27.9 |
Magnesium Binding Sites:
The binding sites of Magnesium atom in the T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Ctp As the Incoming Nucleotide.
(pdb code 1x9s). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Ctp As the Incoming Nucleotide., PDB code: 1x9s:
In total only one binding site of Magnesium was determined in the T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Ctp As the Incoming Nucleotide., PDB code: 1x9s:
Magnesium binding site 1 out of 1 in 1x9s
Go back to
Magnesium Binding Sites List in 1x9s
Magnesium binding site 1 out
of 1 in the T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Ctp As the Incoming Nucleotide.
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Ctp As the Incoming Nucleotide. within 5.0Å range:
|
Reference:
S.Dutta,
Y.Li,
D.Johnson,
L.Dzantiev,
C.C.Richardson,
L.J.Romano,
T.Ellenberger.
Crystal Structures of 2-Acetylaminofluorene and 2-Aminofluorene in Complex with T7 Dna Polymerase Reveal Mechanisms of Mutagenesis. Proc.Natl.Acad.Sci.Usa V. 101 16186 2004.
ISSN: ISSN 0027-8424
PubMed: 15528277
DOI: 10.1073/PNAS.0406516101
Page generated: Mon Dec 14 07:02:54 2020
ISSN: ISSN 0027-8424
PubMed: 15528277
DOI: 10.1073/PNAS.0406516101
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