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Magnesium in PDB 1x9w: T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Atp As the Incoming Nucleotide.

Enzymatic activity of T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Atp As the Incoming Nucleotide.

All present enzymatic activity of T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Atp As the Incoming Nucleotide.:
2.7.7.7;

Protein crystallography data

The structure of T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Atp As the Incoming Nucleotide., PDB code: 1x9w was solved by S.Dutta, Y.Li, D.Johnson, L.Dzantiev, C.C.Richardson, L.J.Romano, T.Ellenberger, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.68 / 2.30
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 105.944, 213.559, 52.168, 90.00, 90.00, 90.00
R / Rfree (%) 23 / 25.8

Magnesium Binding Sites:

The binding sites of Magnesium atom in the T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Atp As the Incoming Nucleotide. (pdb code 1x9w). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Atp As the Incoming Nucleotide., PDB code: 1x9w:

Magnesium binding site 1 out of 1 in 1x9w

Go back to Magnesium Binding Sites List in 1x9w
Magnesium binding site 1 out of 1 in the T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Atp As the Incoming Nucleotide.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of T7 Dna Polymerase in Complex with A Primer/Template Dna Containing A Disordered N-2 Aminofluorene on the Template, Crystallized with Dideoxy-Atp As the Incoming Nucleotide. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg3001

b:54.3
occ:1.00
 OD1 A:ASP5 2.5 25.4 1.0
O A:HOH4013 2.8 31.1 1.0
O A:HOH4082 3.5 31.6 1.0
O A:HOH4039 3.6 19.0 1.0
CG A:ASP5 3.6 23.0 1.0
O A:HIS59 3.7 20.3 1.0
OD1 A:ASP65 3.7 19.7 1.0
OD2 A:ASP65 3.8 22.5 1.0
OD2 A:ASP5 4.2 25.3 1.0
CG A:ASP65 4.2 19.8 1.0
O A:HOH4104 4.4 27.0 1.0
CE A:MET107 4.5 35.5 1.0
O A:ILE6 4.6 26.4 1.0
CB A:ASP5 4.8 22.5 1.0
C A:HIS59 4.9 20.3 1.0

Reference:

S.Dutta, Y.Li, D.Johnson, L.Dzantiev, C.C.Richardson, L.J.Romano, T.Ellenberger. Crystal Structures of 2-Acetylaminofluorene and 2-Aminofluorene in Complex with T7 Dna Polymerase Reveal Mechanisms of Mutagenesis. Proc.Natl.Acad.Sci.Usa V. 101 16186 2004.
ISSN: ISSN 0027-8424
PubMed: 15528277
DOI: 10.1073/PNAS.0406516101
Page generated: Tue Aug 13 17:29:11 2024

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