Magnesium in PDB 2bx2: Catalytic Domain of E. Coli Rnase E

The structure of Catalytic Domain of E. Coli Rnase E, PDB code: 2bx2 was solved by M.J.Marcaida, A.J.Callaghan, W.G.Scott, B.F.Luisi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 2.85
Space group	P 62 2 2
Cell size a, b, c (Å), α, β, γ (°)	195.838, 195.838, 143.569, 90.00, 90.00, 120.00
R / Rfree (%)	23 / 25.7

The structure of Catalytic Domain of E. Coli Rnase E also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Magnesium atom in the Catalytic Domain of E. Coli Rnase E (pdb code 2bx2). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Catalytic Domain of E. Coli Rnase E, PDB code: 2bx2:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Catalytic Domain of E. Coli Rnase E


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Catalytic Domain of E. Coli Rnase E within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg1510 b:45.5 occ:0.50 O L:GLY409 4.0 50.8 1.0 O L:SER295 4.2 50.5 1.0 O L:THR410 4.4 48.7 1.0 C L:THR410 4.7 48.1 1.0 N L:GLY411 4.9 48.2 1.0 CA L:GLY411 5.0 43.9 1.0

Magnesium binding site 2 out of 2 in the Catalytic Domain of E. Coli Rnase E


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Catalytic Domain of E. Coli Rnase E within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg1511 b:62.7 occ:1.00 O L:HOH2021 2.2 63.9 1.0 O L:HOH2022 2.2 63.7 1.0 O L:HOH2033 2.2 65.6 1.0 O L:HOH2032 2.2 62.4 1.0 OD2 L:ASP346 2.7 59.6 1.0 OD2 L:ASP303 2.8 55.5 1.0 CG L:ASP303 3.7 54.7 1.0 CG L:ASP346 3.8 59.1 1.0 OD1 L:ASP303 3.9 57.4 1.0 O L:ILE304 4.1 58.6 1.0 OD1 L:ASP346 4.3 60.9 1.0 O L:ASP346 4.4 54.2 1.0 N L:ILE304 4.8 54.4 1.0 CB L:ASP346 4.9 54.9 1.0

A.J.Callaghan, M.J.Marcaida, J.A.Stead, K.J.Mcdowall, W.G.Scott, B.F.Luisi. Structure of E. Coli Rnase E Catalytic Domain and Implications For Rna Processing and Turnover Nature V. 437 1187 2005.
ISSN: ISSN 0028-0836
PubMed: 16237448
DOI: 10.1038/NATURE04084