Magnesium in PDB 2ik8: Crystal Structure of the Heterodimeric Complex of Human RGS16 and Activated Gi Alpha 1

The structure of Crystal Structure of the Heterodimeric Complex of Human RGS16 and Activated Gi Alpha 1, PDB code: 2ik8 was solved by M.Soundararajan, A.P.Turnbull, E.Papagrigoriou, J.Debreczeni, F.Gorrec, F.Von Delft, J.Weigelt, A.Edwards, C.Arrowsmith, M.Sundstrom, D.A.Doyle, Structural Genomics Consortium (Sgc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.60 / 2.71
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	82.656, 104.312, 124.048, 90.00, 90.00, 90.00
R / Rfree (%)	23.3 / 29.1

The structure of Crystal Structure of the Heterodimeric Complex of Human RGS16 and Activated Gi Alpha 1 also contains other interesting chemical elements:

Fluorine	(F)	8 atoms
Aluminium	(Al)	2 atoms

The binding sites of Magnesium atom in the Crystal Structure of the Heterodimeric Complex of Human RGS16 and Activated Gi Alpha 1 (pdb code 2ik8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Heterodimeric Complex of Human RGS16 and Activated Gi Alpha 1, PDB code: 2ik8:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Heterodimeric Complex of Human RGS16 and Activated Gi Alpha 1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Heterodimeric Complex of Human RGS16 and Activated Gi Alpha 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg356 b:52.3 occ:1.00 OG1 A:THR181 1.7 61.6 1.0 O A:HOH408 1.7 46.8 1.0 OG A:SER47 1.9 59.1 1.0 O A:HOH417 2.0 43.5 1.0 F1 A:ALF355 2.0 48.5 1.0 O1B A:GDP401 2.2 37.1 1.0 CB A:THR181 3.0 61.5 1.0 CB A:SER47 3.0 60.5 1.0 F3 A:ALF355 3.1 48.1 1.0 PB A:GDP401 3.2 40.2 1.0 O2B A:GDP401 3.3 40.0 1.0 AL A:ALF355 3.3 47.6 1.0 CG2 A:THR181 3.9 60.8 1.0 N A:THR181 3.9 60.8 1.0 N A:SER47 3.9 61.3 1.0 CA A:THR181 4.0 61.1 1.0 CA A:SER47 4.0 60.8 1.0 OD2 A:ASP200 4.1 67.4 1.0 O1A A:GDP401 4.2 41.5 1.0 O3A A:GDP401 4.2 40.8 1.0 O A:HOH416 4.3 50.2 1.0 O A:VAL179 4.3 59.4 1.0 O3B A:GDP401 4.4 41.2 1.0 F4 A:ALF355 4.4 46.9 1.0 O2A A:GDP401 4.5 41.2 1.0 PA A:GDP401 4.5 41.6 1.0 O A:VAL201 4.5 62.1 1.0 OD1 A:ASP200 4.6 68.8 1.0 NH1 A:ARG178 4.7 66.4 1.0 CG A:ASP200 4.8 65.5 1.0 F2 A:ALF355 4.9 47.8 1.0 NZ A:LYS46 4.9 61.4 1.0 C A:LYS180 5.0 60.2 1.0 CB A:LYS46 5.0 62.2 1.0 C A:LYS46 5.0 61.8 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Heterodimeric Complex of Human RGS16 and Activated Gi Alpha 1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Heterodimeric Complex of Human RGS16 and Activated Gi Alpha 1 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg356 b:59.6 occ:1.00 F3 C:ALF355 1.7 59.0 1.0 O C:HOH413 1.8 41.9 1.0 OG C:SER47 1.9 61.3 1.0 O1B C:GDP402 1.9 46.0 1.0 O C:HOH405 2.1 40.9 1.0 OG1 C:THR181 2.2 59.5 1.0 CB C:SER47 3.0 61.6 1.0 PB C:GDP402 3.0 46.0 1.0 O2B C:GDP402 3.1 44.8 1.0 CB C:THR181 3.2 59.7 1.0 AL C:ALF355 3.2 58.1 1.0 F2 C:ALF355 3.3 59.3 1.0 N C:SER47 3.8 61.8 1.0 O1A C:GDP402 3.8 46.5 1.0 CA C:SER47 4.0 61.6 1.0 OD2 C:ASP200 4.0 65.3 1.0 O3B C:GDP402 4.0 45.9 1.0 N C:THR181 4.0 59.9 1.0 F1 C:ALF355 4.1 59.1 1.0 O3A C:GDP402 4.2 46.0 1.0 CA C:THR181 4.2 60.0 1.0 CG2 C:THR181 4.2 58.8 1.0 O C:HOH407 4.3 35.1 1.0 PA C:GDP402 4.4 46.0 1.0 O C:VAL201 4.4 61.7 1.0 O2A C:GDP402 4.4 46.6 1.0 O C:VAL179 4.5 59.4 1.0 OD1 C:ASP200 4.6 66.0 1.0 CE C:LYS46 4.7 62.4 1.0 CG C:ASP200 4.7 64.4 1.0 CB C:LYS46 4.8 61.9 1.0 NH1 C:ARG178 4.9 63.7 1.0 F4 C:ALF355 4.9 59.1 1.0 C C:LYS46 4.9 61.7 1.0

M.Soundararajan, F.S.Willard, A.J.Kimple, A.P.Turnbull, L.J.Ball, G.A.Schoch, C.Gileadi, O.Y.Fedorov, E.F.Dowler, V.A.Higman, S.Q.Hutsell, M.Sundstrom, D.A.Doyle, D.P.Siderovski. Structural Diversity in the Rgs Domain and Its Interaction with Heterotrimeric G Protein Alpha-Subunits. Proc.Natl.Acad.Sci.Usa V. 105 6457 2008.
ISSN: ISSN 0027-8424
PubMed: 18434541
DOI: 10.1073/PNAS.0801508105