Magnesium in PDB 2om2: Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14

The structure of Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14, PDB code: 2om2 was solved by D.P.Siderovski, R.J.Kimple, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.20
Space group	P 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	69.592, 82.311, 187.566, 90.00, 90.00, 90.00
R / Rfree (%)	22.7 / 26

The binding sites of Magnesium atom in the Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14 (pdb code 2om2). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14, PDB code: 2om2:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg800 b:51.2 occ:1.00 O2B A:GDP355 2.7 33.8 1.0 O A:HOH4062 2.9 55.4 1.0 O B:HOH4158 3.0 23.3 1.0 O1B A:GDP355 3.4 36.5 1.0 O A:HOH4032 3.5 22.3 1.0 PB A:GDP355 3.6 31.3 1.0 NH2 B:ARG516 4.0 43.9 1.0 CA A:GLY42 4.2 20.0 1.0 N A:GLU43 4.3 20.9 1.0 CZ B:ARG516 4.4 45.0 1.0 O3B A:GDP355 4.4 33.9 1.0 N A:GLY203 4.5 54.4 1.0 NZ A:LYS46 4.6 22.0 1.0 NE B:ARG516 4.6 43.6 1.0 C A:GLY202 4.6 53.2 1.0 O B:GLN508 4.6 27.4 1.0 CA A:GLY203 4.7 55.7 1.0 O A:GLY202 4.8 57.5 1.0 C A:GLY42 4.8 21.7 1.0 OG1 A:THR181 4.9 73.6 1.0 O3A A:GDP355 4.9 36.6 1.0 OG A:SER47 4.9 33.4 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg801 b:35.3 occ:1.00 O1B C:GDP356 2.3 38.0 1.0 O C:LYS1046 2.5 39.5 1.0 OD2 C:ASP1200 2.6 25.5 1.0 OD1 C:ASP1200 3.3 27.6 1.0 CG C:ASP1200 3.3 29.6 1.0 CA C:GLY1202 3.4 44.2 1.0 CE C:LYS1046 3.6 22.5 1.0 C C:LYS1046 3.6 32.9 1.0 O D:HOH4100 3.7 23.4 1.0 PB C:GDP356 3.7 35.4 1.0 CB C:LYS1046 3.8 24.5 1.0 O C:HOH4092 3.8 33.2 1.0 NZ C:LYS1046 3.8 20.2 1.0 CB C:SER1047 4.2 33.8 1.0 N C:GLY1202 4.2 42.5 1.0 CA C:LYS1046 4.4 28.9 1.0 O3B C:GDP356 4.4 34.4 1.0 C C:GLY1202 4.5 45.6 1.0 O3A C:GDP356 4.5 37.3 1.0 O2B C:GDP356 4.6 34.3 1.0 N C:SER1047 4.6 33.0 1.0 CA C:SER1047 4.7 31.0 1.0 CD C:LYS1046 4.7 21.2 1.0 CG C:LYS1046 4.7 25.8 1.0 N C:GLY1203 4.8 48.2 1.0 CB C:ASP1200 4.8 31.4 1.0 OG C:SER1047 4.9 34.1 1.0

D.W.Sammond, Z.M.Eletr, C.Purbeck, R.J.Kimple, D.P.Siderovski, B.Kuhlman. Structure-Based Protocol For Identifying Mutations That Enhance Protein-Protein Binding Affinities. J.Mol.Biol. V. 371 1392 2007.
ISSN: ISSN 0022-2836
PubMed: 17603074
DOI: 10.1016/J.JMB.2007.05.096