Magnesium in PDB 2pi4: T7RNAP Complexed with A PHI10 Protein and Initiating Gtps.

All present enzymatic activity of T7RNAP Complexed with A PHI10 Protein and Initiating Gtps.:
2.7.7.6;

The structure of T7RNAP Complexed with A PHI10 Protein and Initiating Gtps., PDB code: 2pi4 was solved by W.P.Kennedy, J.R.Momand, Y.W.Yin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.04 / 2.50
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	224.450, 73.791, 80.315, 90.00, 90.00, 90.00
R / Rfree (%)	26.5 / 29.7

The binding sites of Magnesium atom in the T7RNAP Complexed with A PHI10 Protein and Initiating Gtps. (pdb code 2pi4). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the T7RNAP Complexed with A PHI10 Protein and Initiating Gtps., PDB code: 2pi4:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the T7RNAP Complexed with A PHI10 Protein and Initiating Gtps.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of T7RNAP Complexed with A PHI10 Protein and Initiating Gtps. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg884 b:23.1 occ:1.00 O3B A:GH3901 3.0 89.8 1.0 O3A A:GH3901 3.6 88.1 1.0 PG A:GH3901 3.9 91.1 1.0 PB A:GH3901 4.0 88.0 1.0 O2G A:GH3901 4.0 90.4 1.0 O1G A:GH3901 4.3 90.3 1.0 O A:HOH911 4.6 69.4 1.0 O A:HOH1017 4.6 69.4 1.0 O A:HOH909 4.6 69.4 1.0 O1B A:GH3901 4.7 87.3 1.0 PA A:GH3901 5.0 88.1 1.0

Magnesium binding site 2 out of 2 in the T7RNAP Complexed with A PHI10 Protein and Initiating Gtps.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of T7RNAP Complexed with A PHI10 Protein and Initiating Gtps. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg885 b:42.4 occ:1.00 OD2 A:ASP537 1.9 84.2 1.0 CG A:ASP537 3.1 84.4 1.0 C3' A:GH3902 3.3 62.7 1.0 C4' A:GH3902 3.5 61.3 1.0 O2B A:GH3902 3.6 69.7 1.0 OD1 A:ASP812 3.6 39.2 1.0 O2' A:GH3902 3.7 64.3 1.0 OD1 A:ASP537 3.9 85.3 1.0 CB A:ASP537 4.0 82.3 1.0 C2' A:GH3902 4.0 63.2 1.0 O5' A:GH3902 4.1 64.6 1.0 C5' A:GH3902 4.2 61.7 1.0 CG A:ASP812 4.4 41.0 1.0 OD2 A:ASP812 4.5 41.0 1.0 O A:GLY538 4.6 79.2 1.0 O4' A:GH3902 4.7 60.5 1.0 PB A:GH3902 4.7 69.0 1.0 O1B A:GH3902 4.8 70.2 1.0

W.P.Kennedy, J.R.Momand, Y.W.Yin. Mechanism For De Novo Rna Synthesis and Initiating Nucleotide Specificity By T7 Rna Polymerase. J.Mol.Biol. V. 370 256 2007.
ISSN: ISSN 0022-2836
PubMed: 17512007
DOI: 10.1016/J.JMB.2007.03.041