Atomistry » Magnesium » PDB 3abl-3aln » 3afb
Atomistry »
  Magnesium »
    PDB 3abl-3aln »
      3afb »

Magnesium in PDB 3afb: Crystal Structures of Catalytic Site Mutants of Active Domain 2 of Chitinase From Pyrococcus Furiosus

Enzymatic activity of Crystal Structures of Catalytic Site Mutants of Active Domain 2 of Chitinase From Pyrococcus Furiosus

All present enzymatic activity of Crystal Structures of Catalytic Site Mutants of Active Domain 2 of Chitinase From Pyrococcus Furiosus:
3.2.1.14;

Protein crystallography data

The structure of Crystal Structures of Catalytic Site Mutants of Active Domain 2 of Chitinase From Pyrococcus Furiosus, PDB code: 3afb was solved by H.Tsuji, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 46.13 / 1.76
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 89.912, 91.997, 107.402, 90.00, 90.00, 90.00
R / Rfree (%) 16.1 / 18.2

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structures of Catalytic Site Mutants of Active Domain 2 of Chitinase From Pyrococcus Furiosus (pdb code 3afb). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structures of Catalytic Site Mutants of Active Domain 2 of Chitinase From Pyrococcus Furiosus, PDB code: 3afb:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 3afb

Go back to Magnesium Binding Sites List in 3afb
Magnesium binding site 1 out of 2 in the Crystal Structures of Catalytic Site Mutants of Active Domain 2 of Chitinase From Pyrococcus Furiosus


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structures of Catalytic Site Mutants of Active Domain 2 of Chitinase From Pyrococcus Furiosus within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1

b:18.4
occ:1.00
OH A:TYR509 3.0 8.1 1.0
O A:HOH49 3.0 8.0 1.0
N A:CYS496 3.4 11.3 1.0
SG A:CYS496 3.6 12.9 1.0
N A:LEU495 3.7 10.5 1.0
CB A:CYS496 3.8 12.4 1.0
CB A:TYR494 3.8 9.9 1.0
CE2 A:TYR509 3.9 7.4 1.0
CB A:LEU495 3.9 10.6 1.0
CB A:PHE487 3.9 6.0 1.0
CZ A:TYR509 3.9 8.5 1.0
CA A:LEU495 4.1 10.7 1.0
CA A:CYS496 4.2 12.2 1.0
C A:LEU495 4.2 11.3 1.0
C A:TYR494 4.3 10.1 1.0
CA A:TYR494 4.5 9.9 1.0
CD2 A:PHE487 4.5 6.6 1.0
CG A:PHE487 4.5 6.1 1.0
CG A:TYR494 4.5 8.2 1.0
CG A:LEU495 4.6 10.6 1.0
CD1 A:LEU495 4.8 8.2 1.0
CD1 A:TYR494 4.8 8.8 1.0
CD1 A:ILE525 4.9 7.0 0.5
CD2 A:PHE523 4.9 7.4 1.0
N A:GLY488 5.0 6.4 1.0

Magnesium binding site 2 out of 2 in 3afb

Go back to Magnesium Binding Sites List in 3afb
Magnesium binding site 2 out of 2 in the Crystal Structures of Catalytic Site Mutants of Active Domain 2 of Chitinase From Pyrococcus Furiosus


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structures of Catalytic Site Mutants of Active Domain 2 of Chitinase From Pyrococcus Furiosus within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg2

b:17.3
occ:1.00
OH B:TYR509 3.0 8.5 1.0
O B:HOH8 3.0 7.8 1.0
N B:CYS496 3.3 9.5 1.0
N B:LEU495 3.6 8.1 1.0
SG B:CYS496 3.7 11.5 1.0
CB B:LEU495 3.8 7.8 1.0
CE2 B:TYR509 3.8 8.1 1.0
CB B:CYS496 3.8 10.5 1.0
CZ B:TYR509 3.9 8.4 1.0
CB B:TYR494 3.9 8.6 1.0
CB B:PHE487 3.9 6.9 1.0
CA B:LEU495 4.1 8.0 1.0
C B:LEU495 4.2 8.8 1.0
CA B:CYS496 4.2 10.8 1.0
C B:TYR494 4.3 8.3 1.0
CA B:TYR494 4.4 8.6 1.0
CD2 B:PHE487 4.5 8.9 1.0
CG B:PHE487 4.5 7.2 1.0
CG B:LEU495 4.6 6.5 1.0
CG B:TYR494 4.6 8.6 1.0
CD1 B:ILE525 4.8 5.6 0.5
CD1 B:LEU495 4.8 6.2 1.0
CD2 B:PHE523 4.9 6.9 1.0
N B:GLY488 4.9 7.9 1.0
C B:PHE487 5.0 7.3 1.0
CD1 B:TYR494 5.0 10.0 1.0

Reference:

H.Tsuji, S.Nishimura, T.Inui, Y.Kado, K.Ishikawa, T.Nakamura, K.Uegaki. Kinetic and Crystallographic Analyses of the Catalytic Domain of Chitinase From Pyrococcus Furiosus- the Role of Conserved Residues in the Active Site Febs J. V. 277 2683 2010.
ISSN: ISSN 1742-464X
PubMed: 20553502
DOI: 10.1111/J.1742-464X.2010.07685.X
Page generated: Mon Dec 14 07:53:03 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy