Magnesium in PDB 3c5h: Crystal Structure of the Ras Homolog Domain of Human GRLF1 (P190RHOGAP)

The structure of Crystal Structure of the Ras Homolog Domain of Human GRLF1 (P190RHOGAP), PDB code: 3c5h was solved by L.Shen, Y.Tong, W.Tempel, F.Mackenzie, C.H.Arrowsmith, A.M.Edwards, C.Bountra, J.Weigelt, A.Bochkarev, H.Park, Structural Genomicsconsortium (Sgc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.26 / 1.80
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	108.959, 108.959, 51.319, 90.00, 90.00, 90.00
R / Rfree (%)	20.5 / 24.7

The binding sites of Magnesium atom in the Crystal Structure of the Ras Homolog Domain of Human GRLF1 (P190RHOGAP) (pdb code 3c5h). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Ras Homolog Domain of Human GRLF1 (P190RHOGAP), PDB code: 3c5h:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Ras Homolog Domain of Human GRLF1 (P190RHOGAP)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Ras Homolog Domain of Human GRLF1 (P190RHOGAP) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:19.1 occ:1.00 O2G A:GNP301 2.0 19.6 1.0 O2B A:GNP301 2.1 15.5 1.0 O A:HOH390 2.1 20.9 1.0 OG A:SER36 2.1 18.6 1.0 O A:HOH389 2.2 15.5 1.0 OE2 A:GLU95 2.2 16.6 1.0 CD A:GLU95 3.1 17.1 1.0 CB A:SER36 3.2 14.2 1.0 PG A:GNP301 3.2 16.9 1.0 PB A:GNP301 3.3 15.8 1.0 N3B A:GNP301 3.4 12.8 1.0 OE1 A:GLU95 3.4 17.1 1.0 N A:SER36 3.8 14.7 1.0 OG A:SER56 3.9 18.3 1.0 O3G A:GNP301 4.0 19.9 1.0 CA A:SER36 4.0 12.9 1.0 O2A A:GNP301 4.2 18.1 1.0 OG1 A:THR97 4.2 19.3 1.0 O1B A:GNP301 4.3 16.4 1.0 O3A A:GNP301 4.3 15.4 1.0 O A:HOH324 4.3 19.8 1.0 O1G A:GNP301 4.5 13.7 1.0 CG A:GLU95 4.5 16.5 1.0 O A:HIS54 4.5 20.4 1.0 CB A:THR97 4.6 14.9 1.0 PA A:GNP301 4.6 18.3 1.0 CE A:LYS35 4.6 15.0 1.0 CB A:LYS35 4.7 15.4 1.0 CG2 A:THR97 4.8 12.8 1.0 O1A A:GNP301 4.9 15.6 1.0 NZ A:LYS35 4.9 13.6 1.0 C A:LYS35 4.9 17.7 1.0 CB A:HIS54 5.0 19.5 1.0 O A:HOH319 5.0 21.1 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Ras Homolog Domain of Human GRLF1 (P190RHOGAP)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Ras Homolog Domain of Human GRLF1 (P190RHOGAP) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:22.4 occ:1.00 O A:HOH314 2.0 17.4 1.0 O A:HOH318 2.0 17.6 1.0 O A:HOH328 2.1 20.2 1.0 O A:HOH312 2.1 17.7 1.0 O A:HOH323 2.2 18.9 1.0 O A:HOH337 2.2 24.6 1.0 O A:LEU8 3.8 22.6 1.0 O A:ASP248 3.9 26.8 1.0 O A:GLU6 4.0 24.7 1.0 CA A:ASP248 4.2 21.5 1.0 O A:ILE247 4.2 20.9 1.0 OD1 A:ASP248 4.2 20.7 1.0 O A:HOH380 4.3 31.9 1.0 C A:ASP248 4.3 23.2 1.0 OE1 A:GLN11 4.3 20.4 1.0 C A:GLU6 4.6 20.2 1.0 O A:LYS249 4.7 33.4 1.0 O A:TYR9 4.7 23.5 1.0 CA A:GLU6 4.7 25.0 1.0 O A:ARG5 4.9 28.4 1.0 CB A:ASP248 4.9 21.1 1.0 CG A:ASP248 5.0 21.2 1.0

L.Shen, Y.Tong, W.Tempel, F.Mackenzie, C.H.Arrowsmith, A.M.Edwards, C.Bountra, J.Weigelt, A.Bochkarev, H.Park. Crystal Structure of the Ras Homolog Domain of Human GRLF1 (P190RHOGAP). To Be Published.