Magnesium in PDB 3iqw: Amppnp Complex of C. Therm. GET3

The structure of Amppnp Complex of C. Therm. GET3, PDB code: 3iqw was solved by G.Bozkurt, K.Wild, I.Sinning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	52.86 / 3.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.570, 105.730, 136.930, 90.00, 90.00, 90.00
R / Rfree (%)	22.8 / 27.1

The structure of Amppnp Complex of C. Therm. GET3 also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Magnesium atom in the Amppnp Complex of C. Therm. GET3 (pdb code 3iqw). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Amppnp Complex of C. Therm. GET3, PDB code: 3iqw:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Amppnp Complex of C. Therm. GET3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Amppnp Complex of C. Therm. GET3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg346 b:9.4 occ:1.00 O A:HOH348 2.0 21.5 1.0 O2B A:ANP1 2.2 14.2 1.0 O2G A:ANP1 2.3 18.2 1.0 OG1 A:THR41 2.3 22.0 1.0 O A:HOH347 2.7 18.6 1.0 O A:HOH2 2.8 13.7 1.0 CB A:THR41 3.2 20.8 1.0 PB A:ANP1 3.4 13.9 1.0 PG A:ANP1 3.6 16.9 1.0 N A:THR41 3.6 18.9 1.0 N3B A:ANP1 3.7 15.8 1.0 OD1 A:ASN68 3.8 35.4 1.0 OD1 A:ASP156 3.9 21.4 1.0 CA A:THR41 3.9 18.7 1.0 O2A A:ANP1 4.1 12.9 1.0 CB A:ASN68 4.3 32.0 1.0 O1B A:ANP1 4.4 12.9 1.0 CG2 A:THR41 4.5 20.8 1.0 OD2 A:ASP156 4.5 19.7 1.0 O1G A:ANP1 4.5 16.3 1.0 CG A:ASN68 4.5 35.2 1.0 O3A A:ANP1 4.5 12.1 1.0 PA A:ANP1 4.5 13.0 1.0 O1A A:ANP1 4.6 12.2 1.0 O3G A:ANP1 4.6 17.5 1.0 CG A:ASP156 4.6 20.1 1.0 CB A:LYS40 4.7 19.2 1.0 C A:LYS40 4.7 17.3 1.0 NZ B:LYS35 4.8 48.6 1.0 CE A:LYS40 4.9 23.3 1.0

Magnesium binding site 2 out of 2 in the Amppnp Complex of C. Therm. GET3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Amppnp Complex of C. Therm. GET3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2 b:36.2 occ:1.00 O2B B:ANP1 2.1 27.0 1.0 O2G B:ANP1 2.3 25.6 1.0 OG1 B:THR41 2.4 38.0 1.0 O B:HOH4 2.5 18.8 1.0 O B:HOH5 2.8 30.1 1.0 CB B:THR41 3.2 36.8 1.0 PB B:ANP1 3.3 26.1 1.0 N B:THR41 3.4 34.2 1.0 PG B:ANP1 3.5 25.4 1.0 N3B B:ANP1 3.7 25.8 1.0 CA B:THR41 3.8 35.5 1.0 OD1 B:ASP156 3.9 40.8 1.0 O1B B:ANP1 4.0 24.2 1.0 O3G B:ANP1 4.1 24.6 1.0 OD2 B:ASP156 4.2 38.2 1.0 CB B:LYS40 4.2 33.8 1.0 OD1 B:ASN68 4.3 45.7 1.0 O2A B:ANP1 4.4 26.6 1.0 CG B:ASP156 4.5 39.7 1.0 C B:LYS40 4.5 33.5 1.0 CG2 B:THR41 4.5 38.6 1.0 O1A B:ANP1 4.6 27.1 1.0 O3A B:ANP1 4.7 26.4 1.0 CE B:LYS40 4.7 35.6 1.0 O1G B:ANP1 4.7 24.7 1.0 CA B:LYS40 4.8 32.9 1.0 CB B:ASN68 4.8 46.4 1.0 PA B:ANP1 4.8 27.3 1.0 N B:LYS40 4.8 32.4 1.0 CG B:ASN68 5.0 47.1 1.0

G.Bozkurt, G.Stjepanovic, F.Vilardi, S.Amlacher, K.Wild, G.Bange, V.Favaloro, K.Rippe, E.Hurt, B.Dobberstein, I.Sinning. Structural Insights Into Tail-Anchored Protein Binding and Membrane Insertion By GET3. Proc.Natl.Acad.Sci.Usa V. 106 21131 2009.
ISSN: ISSN 0027-8424
PubMed: 19948960
DOI: 10.1073/PNAS.0910223106