Magnesium in PDB 4f38: Crystal Structure of Geranylgeranylated Rhoa in Complex with Rhogdi in Its Active Gppnhp-Bound Form

The structure of Crystal Structure of Geranylgeranylated Rhoa in Complex with Rhogdi in Its Active Gppnhp-Bound Form, PDB code: 4f38 was solved by Z.Guo, Z.Tnimov, K.Alexandrov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	68.01 / 2.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.000, 71.576, 136.030, 90.00, 90.00, 90.00
R / Rfree (%)	20.2 / 28.1

The binding sites of Magnesium atom in the Crystal Structure of Geranylgeranylated Rhoa in Complex with Rhogdi in Its Active Gppnhp-Bound Form (pdb code 4f38). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Geranylgeranylated Rhoa in Complex with Rhogdi in Its Active Gppnhp-Bound Form, PDB code: 4f38:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Geranylgeranylated Rhoa in Complex with Rhogdi in Its Active Gppnhp-Bound Form


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Geranylgeranylated Rhoa in Complex with Rhogdi in Its Active Gppnhp-Bound Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:25.5 occ:1.00 O2G A:GNP204 1.8 49.3 1.0 OG1 A:THR19 2.3 21.4 1.0 O A:THR37 2.3 25.6 1.0 O2B A:GNP204 2.4 35.5 1.0 O A:HOH301 2.9 8.7 1.0 PG A:GNP204 2.9 52.5 1.0 O1G A:GNP204 3.3 53.8 1.0 OD1 A:ASP59 3.4 24.1 1.0 PB A:GNP204 3.5 35.9 1.0 O A:THR60 3.5 30.8 1.0 C A:THR37 3.6 25.2 1.0 N3B A:GNP204 3.6 44.4 1.0 CB A:THR19 3.7 23.8 1.0 OD2 A:ASP59 3.9 29.6 1.0 N A:THR19 4.0 25.6 1.0 O1B A:GNP204 4.0 34.0 1.0 CG A:ASP59 4.1 27.7 1.0 NZ A:LYS18 4.2 24.7 1.0 O3G A:GNP204 4.2 55.7 1.0 CE A:LYS18 4.3 23.7 1.0 CA A:THR19 4.3 24.3 1.0 N A:VAL38 4.4 24.9 1.0 CA A:VAL38 4.5 23.8 1.0 CB A:LYS18 4.5 26.2 1.0 CA A:THR37 4.5 25.0 1.0 O2A A:GNP204 4.5 32.0 1.0 CG2 A:THR19 4.5 23.5 1.0 C A:THR60 4.7 31.0 1.0 O3A A:GNP204 4.7 32.2 1.0 O A:PRO36 4.8 26.0 1.0 CG2 A:THR37 4.9 23.8 1.0 CG2 A:VAL38 5.0 22.5 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Geranylgeranylated Rhoa in Complex with Rhogdi in Its Active Gppnhp-Bound Form


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Geranylgeranylated Rhoa in Complex with Rhogdi in Its Active Gppnhp-Bound Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg203 b:4.6 occ:1.00 OE1 A:GLU54 1.3 24.9 1.0 OD1 A:ASP45 1.8 24.4 1.0 CD A:GLU54 2.5 27.8 1.0 CG A:ASP45 2.7 30.7 1.0 OD2 A:ASP45 2.9 29.9 1.0 O A:HOH335 2.9 19.5 1.0 OE2 A:GLU54 3.2 27.2 1.0 CG A:GLU54 3.5 29.5 1.0 CB A:ASP45 4.2 31.6 1.0 O A:MET1 4.7 39.5 1.0 CB A:GLU54 4.8 30.7 1.0 CA A:ASP45 4.9 31.6 1.0 CG2 A:VAL43 4.9 23.8 1.0 N A:ASP45 5.0 30.8 1.0

Z.Tnimov, Z.Guo, Y.Gambin, U.T.Nguyen, Y.W.Wu, D.Abankwa, A.Stigter, B.M.Collins, H.Waldmann, R.S.Goody, K.Alexandrov. Quantitative Analysis of Prenylated Rhoa Interaction with Its Chaperone, Rhogdi. J.Biol.Chem. V. 287 26549 2012.
ISSN: ISSN 0021-9258
PubMed: 22628549
DOI: 10.1074/JBC.M112.371294