Magnesium in PDB 4pjj: Myosin VI (Md-INSERT2-Cam, Delta-INSERT1) Post-Rigor State - Long Soaking with PO4

The structure of Myosin VI (Md-INSERT2-Cam, Delta-INSERT1) Post-Rigor State - Long Soaking with PO4, PDB code: 4pjj was solved by T.Isabet, H.Benisty, P.Llinas, H.L.Sweeney, A.Houdusse, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.74 / 2.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	73.010, 109.240, 180.360, 90.00, 90.00, 90.00
R / Rfree (%)	17.2 / 20.7

The structure of Myosin VI (Md-INSERT2-Cam, Delta-INSERT1) Post-Rigor State - Long Soaking with PO4 also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Magnesium atom in the Myosin VI (Md-INSERT2-Cam, Delta-INSERT1) Post-Rigor State - Long Soaking with PO4 (pdb code 4pjj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Myosin VI (Md-INSERT2-Cam, Delta-INSERT1) Post-Rigor State - Long Soaking with PO4, PDB code: 4pjj:

Magnesium binding site 1 out of 1 in the Myosin VI (Md-INSERT2-Cam, Delta-INSERT1) Post-Rigor State - Long Soaking with PO4


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Myosin VI (Md-INSERT2-Cam, Delta-INSERT1) Post-Rigor State - Long Soaking with PO4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg902 b:43.0 occ:1.00 O A:HOH1054 2.0 35.9 1.0 OG A:SER204 2.1 52.9 1.0 O A:HOH1053 2.1 36.2 1.0 O2B A:ADP901 2.2 39.1 1.0 OG1 A:THR158 2.3 38.2 1.0 O A:HOH1061 2.3 43.1 1.0 CB A:SER204 3.0 41.5 1.0 PB A:ADP901 3.3 37.5 1.0 CB A:THR158 3.4 40.1 1.0 O1B A:ADP901 3.4 37.6 1.0 N A:SER204 3.5 38.2 1.0 CA A:SER204 3.8 37.9 1.0 O1A A:ADP901 4.1 44.5 1.0 N A:THR158 4.3 36.0 1.0 CG2 A:THR158 4.3 33.5 1.0 O3A A:ADP901 4.4 37.8 1.0 OD1 A:ASP456 4.4 61.2 1.0 CA A:THR158 4.4 36.1 1.0 O A:HOH1099 4.4 51.0 1.0 O A:HOH1185 4.4 58.5 1.0 O3B A:ADP901 4.5 37.4 1.0 OD2 A:ASP456 4.5 68.3 1.0 O A:ASN202 4.5 41.8 1.0 C A:SER203 4.6 42.0 1.0 ND2 A:ASN200 4.6 37.8 1.0 PA A:ADP901 4.7 41.6 1.0 O A:HOH1144 4.8 49.3 1.0 CG A:ASP456 4.9 59.8 1.0 CA A:SER203 4.9 39.4 1.0 O2A A:ADP901 4.9 45.3 1.0 NZ A:LYS157 5.0 36.2 1.0 CE A:LYS157 5.0 37.2 1.0 C A:SER204 5.0 42.0 1.0

P.Llinas, T.Isabet, L.Song, V.Ropars, B.Zong, H.Benisty, S.Sirigu, C.Morris, C.Kikuti, D.Safer, H.L.Sweeney, A.Houdusse. How Actin Initiates the Motor Activity of Myosin Dev.Cell V. 33 1 2015.
ISSN: ISSN 1534-5807
DOI: 10.1016/J.BPJ.2014.11.160