Atomistry » Magnesium » PDB 4uux-4w5n » 4v2i
Atomistry »
  Magnesium »
    PDB 4uux-4w5n »
      4v2i »

Magnesium in PDB 4v2i: Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp

Enzymatic activity of Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp

All present enzymatic activity of Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp:
3.1.1.1;

Protein crystallography data

The structure of Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp, PDB code: 4v2i was solved by C.D.Santi, H.-K.S.Leiros, A.D.Scala, D.D.Pascale, B.Altermark, N.-P.Willassen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.87 / 1.69
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 73.326, 85.429, 91.747, 90.00, 90.00, 90.00
R / Rfree (%) 14.3 / 18.2

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp (pdb code 4v2i). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp, PDB code: 4v2i:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in 4v2i

Go back to Magnesium Binding Sites List in 4v2i
Magnesium binding site 1 out of 3 in the Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1317

b:17.0
occ:1.00
 H A:ALA252 2.2 9.5 1.0
HA A:ARG258 2.6 10.0 1.0
HB A:THR251 2.7 9.5 1.0
HB3 A:TYR186 2.8 9.9 1.0
OD2 A:ASP255 2.8 10.0 1.0
HA A:THR251 2.9 9.5 1.0
N A:ALA252 3.0 7.9 1.0
O A:HOH2329 3.1 9.3 1.0
HG23 A:THR251 3.2 11.6 1.0
HB2 A:TYR186 3.2 9.9 1.0
HA A:TYR186 3.3 9.9 1.0
CB A:THR251 3.3 8.0 1.0
CB A:TYR186 3.4 8.3 1.0
CA A:THR251 3.4 7.9 1.0
CA A:ARG258 3.5 8.3 1.0
HB3 A:ASP255 3.6 11.8 1.0
HB1 A:ALA252 3.7 9.8 1.0
CG A:ASP255 3.7 9.4 1.0
CG2 A:THR251 3.7 9.7 1.0
HB2 A:ARG258 3.8 10.0 1.0
C A:THR251 3.8 7.9 1.0
N A:ARG258 3.8 8.6 1.0
CA A:TYR186 3.9 8.2 1.0
O A:ALA252 3.9 8.1 1.0
HD1 A:HIS285 3.9 11.4 1.0
C A:LEU257 4.0 8.6 1.0
CA A:ALA252 4.1 8.0 1.0
HG3 A:ARG258 4.1 10.6 1.0
CB A:ASP255 4.1 9.8 1.0
HB2 A:LEU257 4.1 13.6 1.0
CB A:ARG258 4.1 8.3 1.0
O A:LEU257 4.1 8.5 1.0
HG22 A:THR251 4.1 11.6 1.0
H A:ARG258 4.1 10.3 1.0
CB A:ALA252 4.3 8.1 1.0
HB2 A:ASP255 4.3 11.8 1.0
C A:ALA252 4.4 8.0 1.0
HB3 A:ALA252 4.5 9.8 1.0
HG21 A:THR251 4.5 11.6 1.0
C A:ARG258 4.6 8.4 1.0
OD1 A:ASP255 4.6 10.5 1.0
CG A:ARG258 4.6 8.9 1.0
OG1 A:THR251 4.6 8.7 1.0
C A:TYR186 4.7 8.4 1.0
O A:ARG258 4.7 8.2 1.0
CG A:TYR186 4.7 8.4 1.0
ND1 A:HIS285 4.7 9.5 1.0
O A:ILE250 4.7 7.9 1.0
HG1 A:THR251 4.7 10.5 1.0
CB A:LEU257 4.8 11.4 1.0
HB3 A:LEU257 4.8 13.6 1.0
N A:THR251 4.8 9.6 1.0
HD2 A:TYR186 4.8 10.0 1.0
HD2 A:PRO187 4.8 9.9 1.0
HA A:ALA252 4.9 9.5 1.0
HA3 A:GLY261 4.9 11.8 1.0
CA A:LEU257 4.9 8.9 1.0
HE1 A:HIS285 4.9 14.2 1.0
H A:TYR186 5.0 9.8 1.0
HB3 A:ARG258 5.0 10.0 1.0
O A:THR251 5.0 7.8 1.0

Magnesium binding site 2 out of 3 in 4v2i

Go back to Magnesium Binding Sites List in 4v2i
Magnesium binding site 2 out of 3 in the Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg1317

b:17.7
occ:1.00
 O B:HOH2412 2.7 8.8 1.0
O B:HOH2413 2.7 16.9 1.0
O B:VAL275 2.7 11.1 1.0
O A:HOH2444 2.8 8.7 1.0
HG2 A:PRO297 3.1 14.2 1.0
HB B:VAL275 3.1 9.8 1.0
HB2 A:GLU5 3.2 10.7 1.0
HG3 A:GLU5 3.2 12.3 1.0
HG B:SER269 3.2 16.2 1.0
HG2 A:GLU5 3.3 12.3 1.0
H B:VAL275 3.5 10.1 0.4
CG A:GLU5 3.6 10.3 1.0
HB2 B:SER269 3.6 16.6 1.0
OG B:SER269 3.7 13.5 1.0
HG13 B:VAL277 3.7 11.6 1.0
C B:VAL275 3.7 8.0 1.0
HG3 A:PRO297 3.8 14.2 1.0
CB A:GLU5 3.8 8.9 1.0
CG A:PRO297 3.8 11.8 1.0
CB B:VAL275 3.9 8.2 1.0
HA A:GLU5 4.1 11.0 1.0
CA B:VAL275 4.2 8.2 1.0
CB B:SER269 4.2 13.8 1.0
N B:VAL275 4.2 8.4 1.0
HG13 B:VAL275 4.3 11.9 1.0
HA3 A:GLY282 4.3 10.0 1.0
O A:HOH2455 4.4 8.5 1.0
HB2 A:PRO297 4.4 11.2 1.0
HG12 B:VAL277 4.4 11.6 1.0
CG1 B:VAL277 4.5 9.6 1.0
CA A:GLU5 4.6 9.2 1.0
HB3 A:GLU5 4.6 10.7 1.0
HD2 A:PRO297 4.7 10.6 1.0
CG1 B:VAL275 4.7 9.9 1.0
O A:HOH2443 4.7 8.3 1.0
HG22 B:VAL275 4.7 10.0 1.0
CB A:PRO297 4.7 9.3 1.0
HB3 B:SER269 4.7 16.6 1.0
O A:HOH2020 4.8 14.6 1.0
O A:ASN281 4.8 7.9 1.0
HA B:THR276 4.8 9.3 1.0
HD3 A:PRO6 4.8 11.6 1.0
HG11 B:VAL277 4.9 11.6 1.0
CD A:PRO297 4.9 8.8 1.0
N B:THR276 4.9 7.9 1.0
CG2 B:VAL275 5.0 8.3 1.0
HG11 B:VAL275 5.0 11.9 1.0

Magnesium binding site 3 out of 3 in 4v2i

Go back to Magnesium Binding Sites List in 4v2i
Magnesium binding site 3 out of 3 in the Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Biochemical Characterization and Structural Analysis of A New Cold- Active and Salt Tolerant Esterase From the Marine Bacterium Thalassospira Sp within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg1318

b:17.9
occ:1.00
 H B:ALA252 2.2 10.0 1.0
HA B:ARG258 2.6 9.5 1.0
HB B:THR251 2.6 10.6 1.0
HB3 B:TYR186 2.8 9.3 1.0
OD2 B:ASP255 2.8 10.2 1.0
HA B:THR251 2.9 9.1 1.0
N B:ALA252 3.0 8.3 1.0
HG23 B:THR251 3.1 9.3 1.0
O B:HOH2323 3.2 8.2 1.0
CB B:THR251 3.3 8.8 1.0
HB2 B:TYR186 3.3 9.3 1.0
HA B:TYR186 3.3 10.8 1.0
CA B:THR251 3.4 7.6 1.0
CA B:ARG258 3.4 7.9 1.0
CB B:TYR186 3.4 7.8 1.0
HB3 B:ASP255 3.5 10.7 1.0
HB1 B:ALA252 3.6 11.8 1.0
HB2 B:ARG258 3.6 9.5 1.0
CG B:ASP255 3.6 12.2 1.0
CG2 B:THR251 3.7 7.8 1.0
N B:ARG258 3.7 9.3 1.0
C B:THR251 3.7 7.6 1.0
O B:ALA252 3.8 8.0 1.0
CA B:TYR186 3.9 9.0 1.0
HD1 B:HIS285 3.9 9.5 1.0
C B:LEU257 4.0 8.1 1.0
CA B:ALA252 4.0 7.8 1.0
CB B:ARG258 4.0 8.0 1.0
HG22 B:THR251 4.0 9.3 1.0
H B:ARG258 4.1 11.2 1.0
CB B:ASP255 4.1 8.9 1.0
HG3 B:ARG258 4.1 9.6 1.0
HB2 B:LEU257 4.1 11.3 1.0
O B:LEU257 4.1 8.0 1.0
CB B:ALA252 4.2 9.8 1.0
HB2 B:ASP255 4.3 10.7 1.0
HB3 B:ALA252 4.4 11.8 1.0
C B:ALA252 4.4 8.1 1.0
HG21 B:THR251 4.5 9.3 1.0
OD1 B:ASP255 4.5 10.2 1.0
C B:ARG258 4.6 10.0 1.0
OG1 B:THR251 4.6 8.9 1.0
CG B:ARG258 4.6 8.0 1.0
O B:ARG258 4.7 7.9 1.0
C B:TYR186 4.7 7.8 1.0
HG1 B:THR251 4.7 10.7 1.0
ND1 B:HIS285 4.7 7.9 1.0
CG B:TYR186 4.7 8.3 1.0
CB B:LEU257 4.7 9.4 1.0
HB3 B:LEU257 4.8 11.3 1.0
N B:THR251 4.8 10.0 1.0
O B:ILE250 4.8 10.5 1.0
HD2 B:TYR186 4.8 8.9 1.0
HA B:ALA252 4.8 9.3 1.0
HB3 B:ARG258 4.9 9.5 1.0
HA3 B:GLY261 4.9 9.4 1.0
CA B:LEU257 4.9 8.3 1.0
HD2 B:PRO187 4.9 9.0 1.0
H B:TYR186 4.9 8.8 1.0
HE1 B:HIS285 4.9 13.9 1.0
O B:THR251 5.0 9.4 1.0

Reference:

C.De Santi, H.S.Leiros, A.Di Scala, D.De Pascale, B.Altermark, N.Willassen. Biochemical Characterization and Structural Analysis of A New Cold-Active and Salt-Tolerant Esterase From the Marine Bacterium Thalassospira Sp. Extremophiles V. 20 323 2016.
ISSN: ISSN 1431-0651
PubMed: 27016194
DOI: 10.1007/S00792-016-0824-Z
Page generated: Tue Aug 20 05:06:28 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy