Atomistry » Magnesium » PDB 4w5q-4wfn » 4w89
Atomistry »
  Magnesium »
    PDB 4w5q-4wfn »
      4w89 »

Magnesium in PDB 4w89: Crystal Structure of XEG5A, A GH5 Xyloglucan-Specific Endo-Beta-1,4- Glucanase From Metagenomic Library, in Complex with Cellotriose

Enzymatic activity of Crystal Structure of XEG5A, A GH5 Xyloglucan-Specific Endo-Beta-1,4- Glucanase From Metagenomic Library, in Complex with Cellotriose

All present enzymatic activity of Crystal Structure of XEG5A, A GH5 Xyloglucan-Specific Endo-Beta-1,4- Glucanase From Metagenomic Library, in Complex with Cellotriose:
3.2.1.151;

Protein crystallography data

The structure of Crystal Structure of XEG5A, A GH5 Xyloglucan-Specific Endo-Beta-1,4- Glucanase From Metagenomic Library, in Complex with Cellotriose, PDB code: 4w89 was solved by C.R.Santos, R.L.Cordeiro, D.W.S.Wong, M.T.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 43.20 / 2.40
Space group P 31
Cell size a, b, c (Å), α, β, γ (°) 96.704, 96.704, 95.719, 90.00, 90.00, 120.00
R / Rfree (%) 22 / 25.6

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of XEG5A, A GH5 Xyloglucan-Specific Endo-Beta-1,4- Glucanase From Metagenomic Library, in Complex with Cellotriose (pdb code 4w89). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of XEG5A, A GH5 Xyloglucan-Specific Endo-Beta-1,4- Glucanase From Metagenomic Library, in Complex with Cellotriose, PDB code: 4w89:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 4w89

Go back to Magnesium Binding Sites List in 4w89
Magnesium binding site 1 out of 2 in the Crystal Structure of XEG5A, A GH5 Xyloglucan-Specific Endo-Beta-1,4- Glucanase From Metagenomic Library, in Complex with Cellotriose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of XEG5A, A GH5 Xyloglucan-Specific Endo-Beta-1,4- Glucanase From Metagenomic Library, in Complex with Cellotriose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg501

b:48.2
occ:1.00
 O A:HOH618 2.0 40.3 1.0
O A:HOH617 2.0 55.4 1.0
O B:HOH625 2.0 30.7 1.0
O B:HOH626 2.4 37.4 1.0
O A:HOH602 2.5 50.1 1.0
O A:HOH619 2.5 40.8 1.0
OD1 A:ASN278 3.3 39.7 1.0
OD1 B:ASP270 4.1 39.2 1.0
CG A:ASN278 4.4 39.2 1.0
OD1 A:ASP281 4.4 37.2 1.0
CA A:ASN278 4.4 37.1 1.0
O A:GLY277 4.5 34.1 1.0
OD2 A:ASP281 4.5 40.0 1.0
OD2 B:ASP270 4.6 40.3 1.0
CG B:ASP270 4.8 39.2 1.0
O B:HOH648 4.8 35.0 1.0
CG A:ASP281 4.9 37.2 1.0
C A:GLY277 4.9 34.9 1.0
CB A:ASN278 4.9 38.7 1.0
N A:ASN278 4.9 36.4 1.0

Magnesium binding site 2 out of 2 in 4w89

Go back to Magnesium Binding Sites List in 4w89
Magnesium binding site 2 out of 2 in the Crystal Structure of XEG5A, A GH5 Xyloglucan-Specific Endo-Beta-1,4- Glucanase From Metagenomic Library, in Complex with Cellotriose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of XEG5A, A GH5 Xyloglucan-Specific Endo-Beta-1,4- Glucanase From Metagenomic Library, in Complex with Cellotriose within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg501

b:55.6
occ:1.00
 O B:HOH607 1.8 33.8 1.0
O B:HOH628 2.0 50.5 1.0
O A:HOH620 2.0 37.6 1.0
O B:HOH627 2.2 55.1 1.0
O A:HOH621 2.3 33.8 1.0
O B:HOH606 2.9 59.6 1.0
OD1 B:ASN278 3.5 40.5 1.0
OD1 A:ASP270 3.8 37.7 1.0
O B:GLY277 4.2 36.9 1.0
OD1 B:ASP281 4.3 38.7 1.0
CA B:ASN278 4.4 37.1 1.0
OD2 B:ASP281 4.4 40.7 1.0
CG B:ASN278 4.5 39.2 1.0
OD2 A:ASP270 4.5 38.4 1.0
CG A:ASP270 4.6 38.2 1.0
C B:GLY277 4.7 36.3 1.0
CG B:ASP281 4.8 38.8 1.0
N B:ASN278 4.8 36.5 1.0
CB B:ASN278 5.0 38.5 1.0

Reference:

C.R.Santos, R.L.Cordeiro, D.W.Wong, M.T.Murakami. Structural Basis For Xyloglucan Specificity and Alpha-D-Xylp(16)-D-Glcp Recognition at the -1 Subsite Within the GH5 Family. Biochemistry 2015.
ISSN: ISSN 0006-2960
PubMed: 25714929
DOI: 10.1021/ACS.BIOCHEM.5B00011
Page generated: Tue Aug 12 01:10:59 2025

Last articles

Mg in 5HQC
Mg in 5HQB
Mg in 5HQ8
Mg in 5HQA
Mg in 5HQ4
Mg in 5HPY
Mg in 5HPZ
Mg in 5HP1
Mg in 5HPO
Mg in 5HPH
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy