Magnesium in PDB 5vw1: Crystal Structure of SPYCAS9-Sgrna-ACRIIA4 Ternary Complex

The structure of Crystal Structure of SPYCAS9-Sgrna-ACRIIA4 Ternary Complex, PDB code: 5vw1 was solved by H.Yang, D.J.Patel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.02 / 2.60
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	72.194, 101.242, 303.563, 90.00, 90.00, 90.00
R / Rfree (%)	17.8 / 22.6

The binding sites of Magnesium atom in the Crystal Structure of SPYCAS9-Sgrna-ACRIIA4 Ternary Complex (pdb code 5vw1). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of SPYCAS9-Sgrna-ACRIIA4 Ternary Complex, PDB code: 5vw1:

Magnesium binding site 1 out of 1 in the Crystal Structure of SPYCAS9-Sgrna-ACRIIA4 Ternary Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of SPYCAS9-Sgrna-ACRIIA4 Ternary Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg101 b:43.1 occ:1.00 OP1 C:U50 2.4 53.4 1.0 O6 C:G62 3.4 59.0 1.0 P C:U50 3.7 43.2 1.0 OP1 C:A51 4.1 38.2 1.0 OP2 C:U50 4.1 37.6 1.0 OP2 C:A52 4.1 50.8 1.0 O6 C:G53 4.3 49.4 1.0 OP1 C:A52 4.3 57.6 1.0 C6 C:G62 4.3 51.6 1.0 N1 C:G62 4.3 44.2 1.0 O5' C:U50 4.5 32.3 1.0 N7 C:G53 4.6 46.9 1.0 P C:A52 4.8 45.4 1.0 NZ A:LYS1123 4.8 44.4 1.0 C5' C:U50 4.8 33.1 1.0 C6 C:G53 5.0 46.6 1.0 O3' C:A49 5.0 51.6 1.0

H.Yang, D.J.Patel. Inhibition Mechanism of An Anti-Crispr Suppressor ACRIIA4 Targeting SPYCAS9. Mol. Cell V. 67 117 2017.
ISSN: ISSN 1097-4164
PubMed: 28602637
DOI: 10.1016/J.MOLCEL.2017.05.024