Magnesium in PDB 6b3k: Crystal Structure of Mutant Spinach Rna Aptamer in Complex with Fab BL3-6

The structure of Crystal Structure of Mutant Spinach Rna Aptamer in Complex with Fab BL3-6, PDB code: 6b3k was solved by S.Dasgupta, D.Koirala, S.A.Shelke, J.A.Piccirilli, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	69.10 / 2.09
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	147.216, 80.019, 93.405, 90.00, 111.47, 90.00
R / Rfree (%)	19.3 / 22.6

The binding sites of Magnesium atom in the Crystal Structure of Mutant Spinach Rna Aptamer in Complex with Fab BL3-6 (pdb code 6b3k). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of Mutant Spinach Rna Aptamer in Complex with Fab BL3-6, PDB code: 6b3k:

Magnesium binding site 1 out of 1 in the Crystal Structure of Mutant Spinach Rna Aptamer in Complex with Fab BL3-6


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Mutant Spinach Rna Aptamer in Complex with Fab BL3-6 within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg301 b:53.0 occ:1.00 OG L:SER177 3.0 49.7 1.0 N H:SER191 3.3 38.9 1.0 CB H:PHE178 3.5 39.7 1.0 OG H:SER191 3.5 49.6 1.0 CA H:LEU190 3.7 39.0 1.0 CB L:SER163 3.8 44.8 1.0 C H:LEU190 3.9 38.0 1.0 CG H:PHE178 4.0 38.2 1.0 CB H:SER191 4.1 43.6 1.0 CB L:SER177 4.1 40.0 1.0 CA H:SER191 4.2 42.7 1.0 O H:PRO179 4.2 39.2 1.0 OG L:SER163 4.3 45.2 1.0 CB H:LEU190 4.4 39.4 1.0 CD2 H:PHE178 4.4 41.7 1.0 O H:SER191 4.5 39.8 1.0 CA H:PHE178 4.7 39.4 1.0 OG1 L:THR179 4.8 45.7 1.0 N H:LEU190 4.8 36.6 1.0 C H:SER191 4.8 40.7 1.0 CD1 H:PHE178 4.9 38.0 1.0 O H:SER189 4.9 38.5 1.0 C H:PHE178 4.9 40.0 1.0 O H:PHE178 5.0 41.3 1.0 O H:LEU190 5.0 40.3 1.0

D.Koirala, S.A.Shelke, M.Dupont, S.Ruiz, S.Dasgupta, L.J.Bailey, S.A.Benner, J.A.Piccirilli. Affinity Maturation of A Portable Fab-Rna Module For Chaperone-Assisted Rna Crystallography. Nucleic Acids Res. V. 46 2624 2018.
ISSN: ESSN 1362-4962
PubMed: 29309709
DOI: 10.1093/NAR/GKX1292