Magnesium in PDB 6lx1: Potato D-Enzyme Complexed with Acarbose

All present enzymatic activity of Potato D-Enzyme Complexed with Acarbose:
2.4.1.25;

The structure of Potato D-Enzyme Complexed with Acarbose, PDB code: 6lx1 was solved by H.Unno, K.Imamura, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	59.96 / 2.03
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	69.171, 120.293, 174.698, 90.00, 90.00, 90.00
R / Rfree (%)	16.3 / 20.6

The structure of Potato D-Enzyme Complexed with Acarbose also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

The binding sites of Magnesium atom in the Potato D-Enzyme Complexed with Acarbose (pdb code 6lx1). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Potato D-Enzyme Complexed with Acarbose, PDB code: 6lx1:

Magnesium binding site 1 out of 1 in the Potato D-Enzyme Complexed with Acarbose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Potato D-Enzyme Complexed with Acarbose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg604 b:37.8 occ:0.50 NE2 A:HIS195 2.0 27.2 1.0 OE2 A:GLU189 2.1 29.8 1.0 CE1 A:HIS195 2.7 24.9 1.0 CD A:GLU189 2.8 35.0 1.0 CD2 A:HIS195 2.9 23.8 1.0 OE1 A:GLU189 2.9 42.9 1.0 ND1 A:HIS195 3.8 25.6 1.0 CG A:HIS195 3.9 22.9 1.0 CB A:ASN193 4.3 24.8 1.0 CG A:GLU189 4.3 34.4 1.0 ND2 A:ASN193 4.5 32.6 1.0 O A:GLU189 4.7 21.2 1.0 CG A:ASN193 5.0 27.2 1.0

K.Imamura, T.Matsuura, A.Nakagawa, S.Kitamura, M.Kusunoki, T.Takaha, H.Unno. Structural Analysis and Reaction Mechanism of the Disproportionating Enzyme (D-Enzyme) From Potato. Protein Sci. V. 29 2085 2020.
ISSN: ESSN 1469-896X
PubMed: 32808707
DOI: 10.1002/PRO.3932