Magnesium in PDB 7xm0: Crystal Structure of SAU3AI-C and Dna Substrate Complex

All present enzymatic activity of Crystal Structure of SAU3AI-C and Dna Substrate Complex:
3.1.21.4;

The structure of Crystal Structure of SAU3AI-C and Dna Substrate Complex, PDB code: 7xm0 was solved by L.Yahui, Y.Feng, H.Jianhua, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.23 / 2.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	62.943, 98.391, 209.455, 90, 90, 90
R / Rfree (%)	18.8 / 23.6

The binding sites of Magnesium atom in the Crystal Structure of SAU3AI-C and Dna Substrate Complex (pdb code 7xm0). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure of SAU3AI-C and Dna Substrate Complex, PDB code: 7xm0:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystal Structure of SAU3AI-C and Dna Substrate Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of SAU3AI-C and Dna Substrate Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:41.0 occ:1.00 OD1 A:ASN418 2.0 64.3 1.0 OD1 A:ASP422 2.4 79.8 1.0 OP2 E:DG4 2.5 47.1 1.0 O A:ASN418 2.9 62.5 1.0 O A:PHE420 3.1 52.1 1.0 CG A:ASN418 3.2 50.6 1.0 P E:DG4 3.4 55.6 1.0 CG A:ASP422 3.4 57.1 1.0 OP1 E:DG4 3.4 64.3 1.0 OD2 A:ASP422 3.6 71.4 1.0 C A:ASN418 3.6 47.0 1.0 N A:PHE420 3.8 51.2 1.0 ND2 A:ASN418 3.9 64.1 1.0 C A:PHE420 3.9 54.0 1.0 CA A:PHE420 4.2 50.8 1.0 CB A:PHE420 4.3 52.7 1.0 CA A:ASN418 4.3 50.0 1.0 CB A:ASN418 4.3 53.9 1.0 N A:ASN418 4.4 55.0 1.0 C A:ASN419 4.4 51.0 1.0 N A:ASN419 4.4 46.1 1.0 O3' E:DT3 4.5 58.2 1.0 C3' E:DT3 4.5 44.0 1.0 O5' E:DG4 4.6 50.3 1.0 CA A:ASN419 4.6 46.6 1.0 O A:VAL421 4.7 61.3 1.0 CB A:ASP422 4.8 55.2 1.0 C A:VAL421 4.9 55.1 1.0

Magnesium binding site 2 out of 3 in the Crystal Structure of SAU3AI-C and Dna Substrate Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of SAU3AI-C and Dna Substrate Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg501 b:33.4 occ:1.00 OD1 B:ASN418 2.3 55.9 1.0 OP2 G:DG4 2.6 45.8 1.0 OD2 B:ASP422 2.7 85.4 1.0 O B:ASN418 2.8 42.1 1.0 O B:PHE420 2.9 49.5 1.0 CG B:ASN418 3.3 48.0 1.0 CG B:ASP422 3.5 54.7 1.0 P G:DG4 3.6 53.3 1.0 C B:ASN418 3.6 43.2 1.0 N B:PHE420 3.7 43.1 1.0 OD1 B:ASP422 3.7 73.8 1.0 C B:PHE420 3.7 53.9 1.0 OP1 G:DG4 3.8 60.1 1.0 O G:HOH103 4.0 46.3 1.0 ND2 B:ASN418 4.0 38.9 1.0 CA B:PHE420 4.0 43.0 1.0 CB B:PHE420 4.1 44.0 1.0 O B:VAL421 4.3 59.0 1.0 CA B:ASN418 4.4 51.0 1.0 N B:ASN418 4.4 45.3 1.0 C B:ASN419 4.4 47.0 1.0 CB B:ASN418 4.4 48.3 1.0 N B:ASN419 4.5 37.4 1.0 CA B:ASN419 4.7 38.9 1.0 C B:VAL421 4.7 61.6 1.0 O3' G:DT3 4.7 54.2 1.0 O5' G:DG4 4.7 62.4 1.0 C3' G:DT3 4.8 57.8 1.0 CB B:ASP422 4.9 63.2 1.0 N B:VAL421 4.9 54.1 1.0

Magnesium binding site 3 out of 3 in the Crystal Structure of SAU3AI-C and Dna Substrate Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of SAU3AI-C and Dna Substrate Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg501 b:46.5 occ:1.00 OD1 C:ASN418 1.9 90.4 1.0 OD1 C:ASP422 2.6 76.0 1.0 OP2 H:DG4 2.6 54.8 1.0 O C:ASN418 2.9 66.2 1.0 O C:PHE420 3.0 64.5 1.0 CG C:ASN418 3.1 73.8 1.0 CG C:ASP422 3.4 78.6 1.0 OP1 H:DG4 3.4 71.8 1.0 P H:DG4 3.5 65.8 1.0 OD2 C:ASP422 3.5 73.1 1.0 C C:ASN418 3.6 67.0 1.0 N C:PHE420 3.7 63.4 1.0 C C:PHE420 3.8 73.1 1.0 ND2 C:ASN418 3.8 72.8 1.0 CA C:PHE420 4.1 72.6 1.0 CB C:PHE420 4.2 77.9 1.0 CB C:ASN418 4.2 64.5 1.0 CA C:ASN418 4.3 66.0 1.0 C C:ASN419 4.3 69.4 1.0 N C:ASN418 4.4 60.2 1.0 N C:ASN419 4.4 65.0 1.0 O C:VAL421 4.6 68.6 1.0 O3' H:DT3 4.6 70.5 1.0 CA C:ASN419 4.6 63.4 1.0 O5' H:DG4 4.6 64.7 1.0 C3' H:DT3 4.7 61.3 1.0 CB C:ASP422 4.8 74.6 1.0 C C:VAL421 4.8 70.4 1.0 N C:VAL421 4.9 74.7 1.0

L.Yahui, Y.Feng, H.Jianhua. The Self-Activation Mechanism of Type Iie Restriction Endonuclease SAU3AI To Be Published.