Magnesium in PDB 8e70: Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part
Other elements in 8e70:
The structure of Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part also contains other interesting chemical elements:
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part
(pdb code 8e70). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the
Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part, PDB code: 8e70:
Jump to Magnesium binding site number:
1;
2;
3;
4;
5;
6;
Magnesium binding site 1 out
of 6 in 8e70
Go back to
Magnesium Binding Sites List in 8e70
Magnesium binding site 1 out
of 6 in the Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
e:Mg1001
b:157.0
occ:1.00
|
F3
|
e:BEF1002
|
1.9
|
157.0
|
1.0
|
O2B
|
e:ADP1000
|
2.2
|
188.5
|
1.0
|
BE
|
e:BEF1002
|
3.2
|
164.8
|
1.0
|
PB
|
e:ADP1000
|
3.3
|
191.2
|
1.0
|
OG1
|
e:THR185
|
3.3
|
21.8
|
1.0
|
O1B
|
e:ADP1000
|
3.3
|
168.3
|
1.0
|
CE
|
e:LYS184
|
3.3
|
23.4
|
1.0
|
NZ
|
e:LYS184
|
3.5
|
23.4
|
1.0
|
F2
|
e:BEF1002
|
3.8
|
177.2
|
1.0
|
CB
|
e:LYS184
|
3.9
|
23.4
|
1.0
|
O1A
|
e:ADP1000
|
3.9
|
210.7
|
1.0
|
N
|
e:THR185
|
4.1
|
21.8
|
1.0
|
F1
|
e:BEF1002
|
4.3
|
172.7
|
1.0
|
OD2
|
e:ASP265
|
4.4
|
21.4
|
1.0
|
O3B
|
e:ADP1000
|
4.4
|
178.7
|
1.0
|
O3A
|
e:ADP1000
|
4.4
|
230.8
|
1.0
|
CD
|
e:LYS184
|
4.5
|
23.4
|
1.0
|
PA
|
e:ADP1000
|
4.5
|
227.2
|
1.0
|
NH2
|
f:ARG366
|
4.5
|
25.5
|
1.0
|
CB
|
e:THR185
|
4.5
|
21.8
|
1.0
|
C
|
e:LYS184
|
4.6
|
23.4
|
1.0
|
CA
|
e:THR185
|
4.6
|
21.8
|
1.0
|
CG
|
e:LYS184
|
4.7
|
23.4
|
1.0
|
CA
|
e:LYS184
|
4.7
|
23.4
|
1.0
|
O2A
|
e:ADP1000
|
4.8
|
197.9
|
1.0
|
OD1
|
e:ASP265
|
4.9
|
21.4
|
1.0
|
|
Magnesium binding site 2 out
of 6 in 8e70
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Magnesium Binding Sites List in 8e70
Magnesium binding site 2 out
of 6 in the Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
f:Mg1001
b:157.0
occ:1.00
|
F3
|
f:BEF1002
|
1.8
|
157.0
|
1.0
|
O2B
|
f:ADP1000
|
2.2
|
188.5
|
1.0
|
BE
|
f:BEF1002
|
3.2
|
164.8
|
1.0
|
PB
|
f:ADP1000
|
3.3
|
191.2
|
1.0
|
OG1
|
f:THR185
|
3.3
|
21.8
|
1.0
|
O1B
|
f:ADP1000
|
3.3
|
168.3
|
1.0
|
CE
|
f:LYS184
|
3.3
|
23.4
|
1.0
|
NZ
|
f:LYS184
|
3.5
|
23.4
|
1.0
|
F2
|
f:BEF1002
|
3.8
|
177.2
|
1.0
|
CB
|
f:LYS184
|
3.9
|
23.4
|
1.0
|
O1A
|
f:ADP1000
|
3.9
|
210.7
|
1.0
|
N
|
f:THR185
|
4.1
|
21.8
|
1.0
|
F1
|
f:BEF1002
|
4.3
|
172.7
|
1.0
|
OD2
|
f:ASP265
|
4.4
|
21.4
|
1.0
|
O3B
|
f:ADP1000
|
4.4
|
178.7
|
1.0
|
O3A
|
f:ADP1000
|
4.4
|
230.8
|
1.0
|
CD
|
f:LYS184
|
4.5
|
23.4
|
1.0
|
PA
|
f:ADP1000
|
4.5
|
227.2
|
1.0
|
CB
|
f:THR185
|
4.5
|
21.8
|
1.0
|
NH2
|
a:ARG366
|
4.6
|
25.5
|
1.0
|
C
|
f:LYS184
|
4.6
|
23.4
|
1.0
|
CA
|
f:THR185
|
4.6
|
21.8
|
1.0
|
CG
|
f:LYS184
|
4.7
|
23.4
|
1.0
|
CA
|
f:LYS184
|
4.7
|
23.4
|
1.0
|
O2A
|
f:ADP1000
|
4.8
|
197.9
|
1.0
|
OD1
|
f:ASP265
|
4.9
|
21.4
|
1.0
|
|
Magnesium binding site 3 out
of 6 in 8e70
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Magnesium Binding Sites List in 8e70
Magnesium binding site 3 out
of 6 in the Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
c:Mg1001
b:157.0
occ:1.00
|
F3
|
c:BEF1002
|
1.8
|
157.0
|
1.0
|
O3B
|
c:ADP1000
|
2.2
|
188.5
|
1.0
|
BE
|
c:BEF1002
|
3.2
|
164.8
|
1.0
|
PB
|
c:ADP1000
|
3.3
|
191.2
|
1.0
|
OG1
|
c:THR185
|
3.3
|
21.8
|
1.0
|
O2B
|
c:ADP1000
|
3.3
|
168.3
|
1.0
|
CE
|
c:LYS184
|
3.3
|
23.4
|
1.0
|
NZ
|
c:LYS184
|
3.5
|
23.4
|
1.0
|
F2
|
c:BEF1002
|
3.8
|
177.2
|
1.0
|
CB
|
c:LYS184
|
3.9
|
23.4
|
1.0
|
O2A
|
c:ADP1000
|
3.9
|
210.7
|
1.0
|
N
|
c:THR185
|
4.1
|
21.8
|
1.0
|
F1
|
c:BEF1002
|
4.3
|
172.7
|
1.0
|
OD2
|
c:ASP265
|
4.4
|
21.4
|
1.0
|
O1B
|
c:ADP1000
|
4.4
|
178.7
|
1.0
|
O3A
|
c:ADP1000
|
4.4
|
230.8
|
1.0
|
CD
|
c:LYS184
|
4.5
|
23.4
|
1.0
|
PA
|
c:ADP1000
|
4.5
|
227.2
|
1.0
|
CB
|
c:THR185
|
4.5
|
21.8
|
1.0
|
NH2
|
d:ARG366
|
4.6
|
25.5
|
1.0
|
C
|
c:LYS184
|
4.6
|
23.4
|
1.0
|
CA
|
c:THR185
|
4.6
|
21.8
|
1.0
|
CG
|
c:LYS184
|
4.7
|
23.4
|
1.0
|
CA
|
c:LYS184
|
4.7
|
23.4
|
1.0
|
O1A
|
c:ADP1000
|
4.8
|
197.9
|
1.0
|
OD1
|
c:ASP265
|
4.9
|
21.4
|
1.0
|
|
Magnesium binding site 4 out
of 6 in 8e70
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Magnesium Binding Sites List in 8e70
Magnesium binding site 4 out
of 6 in the Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
b:Mg1001
b:157.0
occ:1.00
|
F3
|
b:BEF1002
|
1.8
|
157.0
|
1.0
|
O1B
|
b:ADP1000
|
2.2
|
188.5
|
1.0
|
BE
|
b:BEF1002
|
3.2
|
164.8
|
1.0
|
PB
|
b:ADP1000
|
3.3
|
191.2
|
1.0
|
OG1
|
b:THR185
|
3.3
|
21.8
|
1.0
|
O2B
|
b:ADP1000
|
3.3
|
168.3
|
1.0
|
CE
|
b:LYS184
|
3.3
|
23.4
|
1.0
|
NZ
|
b:LYS184
|
3.5
|
23.4
|
1.0
|
F2
|
b:BEF1002
|
3.8
|
177.2
|
1.0
|
CB
|
b:LYS184
|
3.9
|
23.4
|
1.0
|
O2A
|
b:ADP1000
|
3.9
|
210.7
|
1.0
|
N
|
b:THR185
|
4.1
|
21.8
|
1.0
|
F1
|
b:BEF1002
|
4.3
|
172.7
|
1.0
|
OD2
|
b:ASP265
|
4.4
|
21.4
|
1.0
|
O3B
|
b:ADP1000
|
4.4
|
178.7
|
1.0
|
O3A
|
b:ADP1000
|
4.4
|
230.8
|
1.0
|
OE2
|
b:GLU211
|
4.4
|
24.4
|
1.0
|
CD
|
b:LYS184
|
4.5
|
23.4
|
1.0
|
PA
|
b:ADP1000
|
4.5
|
227.2
|
1.0
|
CB
|
b:THR185
|
4.5
|
21.8
|
1.0
|
NH2
|
c:ARG366
|
4.6
|
25.5
|
1.0
|
C
|
b:LYS184
|
4.6
|
23.4
|
1.0
|
CA
|
b:THR185
|
4.6
|
21.8
|
1.0
|
CG
|
b:LYS184
|
4.7
|
23.4
|
1.0
|
CA
|
b:LYS184
|
4.7
|
23.4
|
1.0
|
O1A
|
b:ADP1000
|
4.8
|
197.9
|
1.0
|
OD1
|
b:ASP265
|
4.9
|
21.4
|
1.0
|
|
Magnesium binding site 5 out
of 6 in 8e70
Go back to
Magnesium Binding Sites List in 8e70
Magnesium binding site 5 out
of 6 in the Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 5 of Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
a:Mg1001
b:157.0
occ:1.00
|
F3
|
a:BEF1002
|
1.9
|
157.0
|
1.0
|
O1B
|
a:ADP1000
|
2.2
|
188.5
|
1.0
|
BE
|
a:BEF1002
|
3.2
|
164.8
|
1.0
|
PB
|
a:ADP1000
|
3.3
|
191.2
|
1.0
|
OG1
|
a:THR185
|
3.3
|
21.8
|
1.0
|
O2B
|
a:ADP1000
|
3.3
|
168.3
|
1.0
|
CE
|
a:LYS184
|
3.3
|
23.4
|
1.0
|
NZ
|
a:LYS184
|
3.5
|
23.4
|
1.0
|
F2
|
a:BEF1002
|
3.8
|
177.2
|
1.0
|
CB
|
a:LYS184
|
3.9
|
23.4
|
1.0
|
O2A
|
a:ADP1000
|
3.9
|
210.7
|
1.0
|
N
|
a:THR185
|
4.1
|
21.8
|
1.0
|
F1
|
a:BEF1002
|
4.3
|
172.7
|
1.0
|
OD2
|
a:ASP265
|
4.4
|
21.4
|
1.0
|
O3B
|
a:ADP1000
|
4.4
|
178.7
|
1.0
|
O3A
|
a:ADP1000
|
4.4
|
230.8
|
1.0
|
OE2
|
a:GLU211
|
4.4
|
24.4
|
1.0
|
CD
|
a:LYS184
|
4.5
|
23.4
|
1.0
|
PA
|
a:ADP1000
|
4.5
|
227.2
|
1.0
|
CB
|
a:THR185
|
4.5
|
21.8
|
1.0
|
NH2
|
b:ARG366
|
4.6
|
25.5
|
1.0
|
C
|
a:LYS184
|
4.6
|
23.4
|
1.0
|
CA
|
a:THR185
|
4.6
|
21.8
|
1.0
|
CG
|
a:LYS184
|
4.7
|
23.4
|
1.0
|
CA
|
a:LYS184
|
4.7
|
23.4
|
1.0
|
O1A
|
a:ADP1000
|
4.8
|
197.9
|
1.0
|
OD1
|
a:ASP265
|
4.9
|
21.4
|
1.0
|
|
Magnesium binding site 6 out
of 6 in 8e70
Go back to
Magnesium Binding Sites List in 8e70
Magnesium binding site 6 out
of 6 in the Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 6 of Escherichia Coli Rho-Dependent Transcription Pre-Termination Complex Containing 18 Nt Long Rna Spacer, DC75 Rut Mimic Rna, Mg-Adp-BEF3, and Nusg; Rho Hexamer Part within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
d:Mg1001
b:157.0
occ:1.00
|
F3
|
d:BEF1002
|
1.8
|
157.0
|
1.0
|
O3B
|
d:ADP1000
|
2.2
|
188.5
|
1.0
|
BE
|
d:BEF1002
|
3.2
|
164.8
|
1.0
|
PB
|
d:ADP1000
|
3.3
|
191.2
|
1.0
|
OG1
|
d:THR185
|
3.3
|
21.8
|
1.0
|
O2B
|
d:ADP1000
|
3.3
|
168.3
|
1.0
|
CE
|
d:LYS184
|
3.3
|
23.4
|
1.0
|
NZ
|
d:LYS184
|
3.5
|
23.4
|
1.0
|
F2
|
d:BEF1002
|
3.8
|
177.2
|
1.0
|
CB
|
d:LYS184
|
3.9
|
23.4
|
1.0
|
O1A
|
d:ADP1000
|
3.9
|
210.7
|
1.0
|
N
|
d:THR185
|
4.1
|
21.8
|
1.0
|
F1
|
d:BEF1002
|
4.3
|
172.7
|
1.0
|
OD2
|
d:ASP265
|
4.4
|
21.4
|
1.0
|
O1B
|
d:ADP1000
|
4.4
|
178.7
|
1.0
|
O3A
|
d:ADP1000
|
4.4
|
230.8
|
1.0
|
NH2
|
e:ARG366
|
4.4
|
25.5
|
1.0
|
OE2
|
d:GLU211
|
4.4
|
24.4
|
1.0
|
CD
|
d:LYS184
|
4.5
|
23.4
|
1.0
|
PA
|
d:ADP1000
|
4.5
|
227.2
|
1.0
|
CB
|
d:THR185
|
4.5
|
21.8
|
1.0
|
C
|
d:LYS184
|
4.6
|
23.4
|
1.0
|
CA
|
d:THR185
|
4.6
|
21.8
|
1.0
|
CG
|
d:LYS184
|
4.7
|
23.4
|
1.0
|
CA
|
d:LYS184
|
4.7
|
23.4
|
1.0
|
O2A
|
d:ADP1000
|
4.8
|
197.9
|
1.0
|
OD1
|
d:ASP265
|
4.9
|
21.4
|
1.0
|
|
Reference:
V.Molodtsov,
C.Wang,
E.Firlar,
J.T.Kaelber,
R.H.Ebright.
Structural Basis of Rho-Dependent Transcription Termination Biorxiv 2022.
DOI: 10.1101/2022.08.29.505764
Page generated: Fri Oct 4 01:03:22 2024
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