Magnesium in PDB 8zbs: Cryo-Em Structure of Nanodisc-Reconstituted Wildtype Human MRP4 (in Complex with Vincristine)

All present enzymatic activity of Cryo-Em Structure of Nanodisc-Reconstituted Wildtype Human MRP4 (in Complex with Vincristine):
7.6.2.2; 7.6.2.3;

The binding sites of Magnesium atom in the Cryo-Em Structure of Nanodisc-Reconstituted Wildtype Human MRP4 (in Complex with Vincristine) (pdb code 8zbs). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cryo-Em Structure of Nanodisc-Reconstituted Wildtype Human MRP4 (in Complex with Vincristine), PDB code: 8zbs:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cryo-Em Structure of Nanodisc-Reconstituted Wildtype Human MRP4 (in Complex with Vincristine)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of Nanodisc-Reconstituted Wildtype Human MRP4 (in Complex with Vincristine) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1403 b:169.6 occ:1.00 O3G A:ANP1402 2.0 161.2 1.0 N3B A:ANP1402 2.1 162.7 1.0 O3A A:ANP1402 2.2 162.9 1.0 O2A A:ANP1402 2.2 162.9 1.0 PA A:ANP1402 2.4 163.6 1.0 CB A:SER1082 2.4 145.8 1.0 PB A:ANP1402 2.4 165.1 1.0 PG A:ANP1402 2.5 165.0 1.0 OG A:SER1082 2.6 146.7 1.0 O1A A:ANP1402 2.7 160.7 1.0 O1B A:ANP1402 3.0 164.5 1.0 N A:SER1082 3.3 148.0 1.0 CA A:SER1082 3.4 145.9 1.0 O1G A:ANP1402 3.5 162.1 1.0 O2G A:ANP1402 3.7 162.2 1.0 O2B A:ANP1402 3.9 159.3 1.0 O5' A:ANP1402 3.9 161.0 1.0 C A:SER1082 4.1 144.5 1.0 N A:SER1083 4.4 137.8 1.0 C A:LYS1081 4.6 154.8 1.0 N A:LYS1081 4.7 155.4 1.0 O A:SER1082 4.7 144.3 1.0 C5' A:ANP1402 5.0 161.3 1.0

Magnesium binding site 2 out of 2 in the Cryo-Em Structure of Nanodisc-Reconstituted Wildtype Human MRP4 (in Complex with Vincristine)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of Nanodisc-Reconstituted Wildtype Human MRP4 (in Complex with Vincristine) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1405 b:191.9 occ:1.00 OG A:SER452 2.0 187.2 1.0 O1G A:ANP1404 2.0 192.4 1.0 O1B A:ANP1404 2.1 193.2 1.0 N A:SER452 2.5 187.1 1.0 CB A:SER452 2.5 187.6 1.0 O3G A:ANP1404 2.7 193.5 1.0 PG A:ANP1404 2.8 193.3 1.0 CA A:SER452 3.0 186.7 1.0 O2A A:ANP1404 3.2 193.1 1.0 PB A:ANP1404 3.3 195.2 1.0 N3B A:ANP1404 3.6 193.4 1.0 C A:LYS451 3.6 193.4 1.0 O3A A:ANP1404 3.9 193.6 1.0 N A:LYS451 3.9 194.1 1.0 PA A:ANP1404 4.0 193.4 1.0 CB A:LYS451 4.0 194.2 1.0 CA A:LYS451 4.0 194.1 1.0 OD2 A:ASP559 4.2 197.6 1.0 O2G A:ANP1404 4.2 193.3 1.0 OD1 A:ASP559 4.2 197.8 1.0 C A:SER452 4.3 186.4 1.0 O1A A:ANP1404 4.3 193.9 1.0 N A:SER453 4.5 183.9 1.0 O2B A:ANP1404 4.5 193.4 1.0 CE A:LYS451 4.6 194.5 1.0 CG A:ASP559 4.6 197.4 1.0 O A:LYS451 4.7 192.8 1.0 C A:GLY450 4.7 193.3 1.0 CG A:LYS451 4.8 193.9 1.0

Z.Xie, J.Lv, W.Huang, Z.Wu, R.Zhu, Z.Deng, F.Long. Structural Basis For the Reversal of Human MRP4-Mediated Multidrug Resistance By Lapatinib. Cell Rep V. 44 15466 2025.
ISSN: ESSN 2211-1247
PubMed: 40138312
DOI: 10.1016/J.CELREP.2025.115466