Magnesium in PDB 1jub: The K136E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A

All present enzymatic activity of The K136E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A:
1.3.3.1;

The structure of The K136E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A, PDB code: 1jub was solved by S.Norager, S.Arent, O.Bjornberg, M.Ottosen, L.Lo Leggio, K.F.Jensen, S.Larsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	13.00 / 1.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	52.880, 108.250, 65.730, 90.00, 103.74, 90.00
R / Rfree (%)	19.1 / 19.7

The binding sites of Magnesium atom in the The K136E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A (pdb code 1jub). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The K136E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A, PDB code: 1jub:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The K136E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The K136E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg850 b:17.1 occ:1.00 O A:LYS33 2.3 14.5 1.0 O A:SER35 2.4 13.5 1.0 O A:HOH1262 2.4 27.0 1.0 O A:HOH1103 2.4 20.6 1.0 O A:HOH1162 2.5 13.8 1.0 O A:HOH1141 2.6 27.1 1.0 C A:LYS33 3.4 14.1 1.0 C A:SER35 3.6 13.7 1.0 O A:ALA37 4.0 12.1 1.0 C A:ALA34 4.0 14.7 1.0 O A:HOH1099 4.0 18.9 1.0 N A:SER35 4.1 14.3 1.0 O A:ALA34 4.2 15.5 1.0 O A:LEU32 4.2 12.3 1.0 N A:ALA34 4.3 14.2 1.0 CA A:LYS33 4.3 14.2 1.0 CA A:ALA34 4.4 14.5 1.0 O A:HOH1061 4.4 20.4 1.0 CA A:SER35 4.4 13.9 1.0 N A:GLN36 4.5 13.7 1.0 CA A:GLN36 4.6 14.0 1.0 C A:GLN36 4.7 13.5 1.0 N A:ALA37 4.8 12.9 1.0

Magnesium binding site 2 out of 2 in the The K136E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The K136E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg850 b:17.7 occ:1.00 O B:LYS33 2.3 14.9 1.0 O B:SER35 2.4 14.1 1.0 O B:HOH1882 2.4 19.8 1.0 O B:HOH2054 2.4 30.9 1.0 O B:HOH1996 2.5 15.7 1.0 C B:LYS33 3.5 14.5 1.0 C B:SER35 3.6 14.4 1.0 O B:HOH1955 3.9 19.4 1.0 O B:ALA37 4.0 13.2 1.0 C B:ALA34 4.0 15.3 1.0 N B:SER35 4.1 14.7 1.0 O B:ALA34 4.2 15.9 1.0 O B:LEU32 4.3 13.8 1.0 N B:ALA34 4.3 14.9 1.0 CA B:LYS33 4.3 14.6 1.0 O B:HOH1875 4.4 22.0 1.0 CA B:ALA34 4.4 15.2 1.0 CA B:SER35 4.5 14.4 1.0 N B:GLN36 4.5 14.4 1.0 CA B:GLN36 4.6 14.7 1.0 O B:HOH2033 4.6 32.9 1.0 C B:GLN36 4.7 14.2 1.0 N B:ALA37 4.8 13.9 1.0

S.Norager, S.Arent, O.Bjornberg, M.Ottosen, L.Lo Leggio, K.F.Jensen, S.Larsen. Lactococcus Lactis Dihydroorotate Dehydrogenase A Mutants Reveal Important Facets of the Enzymatic Function J.Biol.Chem. V. 278 28812 2003.
ISSN: ISSN 0021-9258
PubMed: 12732650
DOI: 10.1074/JBC.M303767200