Magnesium in PDB 4bl0: Crystal Structure of Yeast BUB3-BUB1 Bound to Phospho-SPC105

All present enzymatic activity of Crystal Structure of Yeast BUB3-BUB1 Bound to Phospho-SPC105:
2.7.11.1;

The structure of Crystal Structure of Yeast BUB3-BUB1 Bound to Phospho-SPC105, PDB code: 4bl0 was solved by I.Primorac, J.R.Weir, A.Musacchio, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.882 / 1.95
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	138.740, 57.900, 118.670, 90.00, 102.53, 90.00
R / Rfree (%)	17.94 / 19.22

The binding sites of Magnesium atom in the Crystal Structure of Yeast BUB3-BUB1 Bound to Phospho-SPC105 (pdb code 4bl0). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Yeast BUB3-BUB1 Bound to Phospho-SPC105, PDB code: 4bl0:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Yeast BUB3-BUB1 Bound to Phospho-SPC105


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Yeast BUB3-BUB1 Bound to Phospho-SPC105 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1341 b:22.2 occ:1.00 O A:ILE257 2.8 24.3 1.0 O A:HOH2071 2.8 43.2 1.0 O A:VAL199 2.8 24.6 1.0 O A:HOH2084 2.9 49.0 1.0 OD2 A:ASP198 2.9 24.3 1.0 OE2 A:GLU258 2.9 40.9 0.6 OD1 A:ASP198 3.4 26.1 1.0 CG A:ASP198 3.4 26.5 1.0 CD A:GLU258 3.6 40.6 1.0 O A:HOH2104 3.8 35.9 1.0 C A:VAL199 3.9 24.6 1.0 C A:ILE257 4.0 22.5 1.0 CB A:ALA200 4.0 24.5 1.0 OE1 A:GLU258 4.2 42.0 0.5 CG A:GLU258 4.4 34.8 1.0 CA A:ALA200 4.4 23.6 1.0 CG1 A:ILE257 4.5 21.8 1.0 N A:ILE257 4.5 18.5 1.0 N A:VAL199 4.6 21.5 1.0 N A:ALA200 4.6 21.9 1.0 CB A:ASP198 4.8 21.8 1.0 CA A:ILE257 4.8 19.8 1.0 N A:GLU258 4.9 22.1 1.0 CA A:VAL199 4.9 21.6 1.0 CA A:GLU258 5.0 26.6 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Yeast BUB3-BUB1 Bound to Phospho-SPC105


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Yeast BUB3-BUB1 Bound to Phospho-SPC105 within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1341 b:17.9 occ:1.00 O D:ILE257 2.8 21.8 1.0 O D:VAL199 2.8 19.8 0.4 O D:VAL199 2.8 19.8 0.6 O D:HOH2082 2.9 38.4 1.0 O D:HOH2067 2.9 41.3 1.0 OD2 D:ASP198 2.9 18.8 0.8 OE2 D:GLU258 2.9 31.9 0.6 OD1 D:ASP198 3.3 22.3 0.8 CG D:ASP198 3.4 21.6 1.0 CD D:GLU258 3.5 33.9 1.0 OE1 D:GLU258 3.8 34.1 0.5 C D:VAL199 3.9 19.6 0.4 C D:VAL199 3.9 19.7 0.6 C D:ILE257 4.0 21.1 1.0 O D:HOH2107 4.1 34.7 1.0 CB D:ALA200 4.1 19.0 1.0 CA D:ALA200 4.4 21.7 1.0 CG D:GLU258 4.5 30.3 1.0 N D:VAL199 4.5 16.5 0.6 N D:VAL199 4.5 16.6 0.4 CG1 D:ILE257 4.6 17.4 1.0 N D:ALA200 4.6 21.1 1.0 N D:ILE257 4.6 18.8 1.0 CB D:ASP198 4.7 17.1 1.0 CA D:VAL199 4.9 18.3 0.4 CA D:VAL199 4.9 18.2 0.6 CA D:ILE257 4.9 19.4 1.0 CG D:ASP157 4.9 23.6 1.0 N D:GLU258 5.0 20.4 1.0 CA D:GLU258 5.0 22.3 1.0

I.Primorac, J.R.Weir, E.Chiroli, F.Gross, I.Hoffmann, S.Van Gerwen, A.Ciliberto, A.Musacchio. BUB3 Reads Phosphorylated Melt Repeats to Promote Spindle Assembly Checkpoint Signaling Elife V. 2 01030 2013.
ISSN: ISSN 2050-084X
PubMed: 24066227
DOI: 10.7554/ELIFE.01030