Magnesium in PDB 4ylp: E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna
Enzymatic activity of E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna
All present enzymatic activity of E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna:
2.7.7.6;
Protein crystallography data
The structure of E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna, PDB code: 4ylp
was solved by
Y.Zuo,
T.A.Steitz,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
39.98 /
5.50
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
237.674,
204.988,
248.838,
90.00,
116.86,
90.00
|
R / Rfree (%)
|
23.1 /
31.3
|
Other elements in 4ylp:
The structure of E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna also contains other interesting chemical elements:
Magnesium Binding Sites:
The binding sites of Magnesium atom in the E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna
(pdb code 4ylp). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the
E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna, PDB code: 4ylp:
Jump to Magnesium binding site number:
1;
2;
3;
Magnesium binding site 1 out
of 3 in 4ylp
Go back to
Magnesium Binding Sites List in 4ylp
Magnesium binding site 1 out
of 3 in the E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mg1503
b:0.5
occ:1.00
|
O3'
|
3:U16
|
1.9
|
0.4
|
1.0
|
OP1
|
3:C17
|
2.2
|
0.2
|
1.0
|
OD1
|
D:ASP460
|
2.2
|
0.6
|
1.0
|
OD1
|
D:ASP464
|
2.2
|
0.7
|
1.0
|
OD1
|
D:ASP462
|
2.4
|
0.2
|
1.0
|
P
|
3:C17
|
2.5
|
0.0
|
1.0
|
OD2
|
D:ASP464
|
2.8
|
0.7
|
1.0
|
CG
|
D:ASP464
|
2.8
|
0.7
|
1.0
|
C3'
|
3:U16
|
3.0
|
0.3
|
1.0
|
C4'
|
3:U16
|
3.2
|
1.0
|
1.0
|
CG
|
D:ASP460
|
3.2
|
0.6
|
1.0
|
CG
|
D:ASP462
|
3.4
|
0.1
|
1.0
|
O
|
D:ASP460
|
3.5
|
0.2
|
1.0
|
O5'
|
3:C17
|
3.6
|
0.8
|
1.0
|
C5'
|
3:U16
|
3.6
|
0.9
|
1.0
|
OP2
|
3:C17
|
3.7
|
0.9
|
1.0
|
C5'
|
3:C17
|
3.7
|
0.3
|
1.0
|
O2'
|
3:U16
|
3.8
|
0.1
|
1.0
|
OD2
|
D:ASP462
|
3.8
|
0.4
|
1.0
|
OD2
|
D:ASP460
|
3.8
|
0.7
|
1.0
|
C2'
|
3:U16
|
4.0
|
0.8
|
1.0
|
C
|
D:ASP460
|
4.1
|
0.6
|
1.0
|
CB
|
D:ASP460
|
4.3
|
0.8
|
1.0
|
CB
|
D:ASP464
|
4.3
|
0.5
|
1.0
|
N
|
D:ASP460
|
4.4
|
0.2
|
1.0
|
CA
|
D:ASP460
|
4.4
|
0.6
|
1.0
|
O5'
|
3:U16
|
4.5
|
0.3
|
1.0
|
O4'
|
3:U16
|
4.6
|
0.9
|
1.0
|
NH2
|
D:ARG425
|
4.6
|
0.3
|
1.0
|
N
|
D:ASP462
|
4.6
|
0.0
|
1.0
|
CB
|
D:ASP462
|
4.7
|
0.8
|
1.0
|
O
|
D:ASP462
|
4.7
|
1.0
|
1.0
|
N
|
D:ASP464
|
4.7
|
0.2
|
1.0
|
C
|
D:ASP462
|
4.8
|
0.0
|
1.0
|
C4'
|
3:C17
|
4.9
|
0.4
|
1.0
|
O4'
|
3:C17
|
4.9
|
0.3
|
1.0
|
CA
|
D:ASP464
|
4.9
|
0.9
|
1.0
|
C1'
|
3:U16
|
5.0
|
0.3
|
1.0
|
CA
|
D:ASP462
|
5.0
|
0.2
|
1.0
|
|
Magnesium binding site 2 out
of 3 in 4ylp
Go back to
Magnesium Binding Sites List in 4ylp
Magnesium binding site 2 out
of 3 in the E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Mg1503
b:0.8
occ:1.00
|
OP1
|
6:C17
|
1.1
|
0.5
|
1.0
|
P
|
6:C17
|
2.2
|
0.1
|
1.0
|
OD1
|
J:ASP462
|
2.3
|
0.8
|
1.0
|
O3'
|
6:U16
|
2.5
|
0.6
|
1.0
|
OD1
|
J:ASP460
|
2.7
|
0.6
|
1.0
|
OD2
|
J:ASP460
|
2.8
|
0.9
|
1.0
|
CG
|
J:ASP462
|
2.9
|
0.0
|
1.0
|
OD2
|
J:ASP462
|
2.9
|
0.9
|
1.0
|
CG
|
J:ASP460
|
3.0
|
0.9
|
1.0
|
OP2
|
6:C17
|
3.1
|
0.9
|
1.0
|
NZ
|
I:LYS1073
|
3.4
|
0.2
|
1.0
|
O5'
|
6:C17
|
3.4
|
0.9
|
1.0
|
OD1
|
J:ASP464
|
3.7
|
0.5
|
1.0
|
C3'
|
6:U16
|
3.7
|
0.6
|
1.0
|
C5'
|
6:C17
|
3.9
|
0.5
|
1.0
|
C5'
|
6:U16
|
4.0
|
0.6
|
1.0
|
C4'
|
6:U16
|
4.1
|
0.9
|
1.0
|
CE
|
I:LYS1073
|
4.2
|
0.9
|
1.0
|
CB
|
J:ASP462
|
4.3
|
0.4
|
1.0
|
OD2
|
J:ASP464
|
4.3
|
0.8
|
1.0
|
CB
|
J:ASP460
|
4.3
|
0.7
|
1.0
|
CG
|
J:ASP464
|
4.4
|
0.2
|
1.0
|
O
|
J:ASP460
|
4.4
|
0.5
|
1.0
|
O5'
|
6:U16
|
4.7
|
0.8
|
1.0
|
NH2
|
I:ARG1106
|
4.7
|
0.8
|
1.0
|
O
|
J:ASP462
|
4.8
|
0.8
|
1.0
|
C
|
J:ASP460
|
4.8
|
0.1
|
1.0
|
N
|
J:ASP462
|
4.9
|
0.2
|
1.0
|
C2'
|
6:U16
|
5.0
|
0.1
|
1.0
|
CA
|
J:ASP462
|
5.0
|
0.7
|
1.0
|
|
Magnesium binding site 3 out
of 3 in 4ylp
Go back to
Magnesium Binding Sites List in 4ylp
Magnesium binding site 3 out
of 3 in the E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of E. Coli Transcription Initiation Complex - 16-Bp Spacer and 5-Nt Rna within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Mg1503
b:1.0
occ:1.00
|
OD2
|
P:ASP462
|
1.6
|
0.5
|
1.0
|
OD2
|
P:ASP460
|
2.4
|
0.5
|
1.0
|
CG
|
P:ASP462
|
2.6
|
0.7
|
1.0
|
OP1
|
9:C17
|
2.6
|
0.8
|
1.0
|
OD1
|
P:ASP462
|
3.0
|
0.3
|
1.0
|
CG
|
P:ASP460
|
3.2
|
0.5
|
1.0
|
NH2
|
O:ARG1106
|
3.2
|
0.4
|
1.0
|
OD1
|
P:ASP460
|
3.6
|
0.8
|
1.0
|
P
|
9:C17
|
3.9
|
0.9
|
1.0
|
CB
|
P:ASP462
|
3.9
|
0.4
|
1.0
|
CE
|
O:LYS1073
|
4.1
|
0.3
|
1.0
|
CZ
|
O:ARG1106
|
4.1
|
0.7
|
1.0
|
OD2
|
O:ASP814
|
4.3
|
1.0
|
1.0
|
CB
|
P:ASP460
|
4.3
|
0.7
|
1.0
|
O3'
|
9:U16
|
4.5
|
0.8
|
1.0
|
OP2
|
9:C17
|
4.5
|
0.6
|
1.0
|
NZ
|
O:LYS1073
|
4.7
|
0.0
|
1.0
|
NE
|
O:ARG1106
|
4.8
|
0.7
|
1.0
|
NH1
|
O:ARG1106
|
4.8
|
0.3
|
1.0
|
N
|
P:ASP462
|
4.9
|
0.0
|
1.0
|
|
Reference:
Y.Zuo,
T.A.Steitz.
Crystal Structures of the E. Coli Transcription Initiation Complexes with A Complete Bubble. Mol.Cell V. 58 534 2015.
ISSN: ISSN 1097-2765
PubMed: 25866247
DOI: 10.1016/J.MOLCEL.2015.03.010
Page generated: Sat Sep 28 23:22:23 2024
|