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Magnesium in PDB 6dpn: Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C, PDB code: 6dpn was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.89 / 1.49
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.501, 37.581, 61.951, 90.00, 96.24, 90.00
R / Rfree (%) 15.9 / 17.9

Other elements in 6dpn:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C also contains other interesting chemical elements:

Potassium (K) 2 atoms
Iodine (I) 4 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C (pdb code 6dpn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C, PDB code: 6dpn:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dpn

Go back to Magnesium Binding Sites List in 6dpn
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:25.1
occ:1.00
O A:HOH339 1.9 26.1 1.0
OD2 A:ASP71 2.0 24.1 1.0
OP1 b:C5 2.0 25.7 1.0
OD1 A:ASP192 2.1 34.2 1.0
O A:HOH340 2.1 26.2 1.0
O A:HOH319 2.2 31.8 1.0
CG A:ASP71 3.0 24.8 1.0
CG A:ASP192 3.2 31.4 1.0
HA A:ASP192 3.3 32.3 1.0
P b:C5 3.3 26.2 1.0
OD1 A:ASP71 3.4 25.6 1.0
H A:TYR193 3.4 33.8 1.0
OP2 b:C5 3.8 28.6 1.0
MG A:MG202 3.8 23.8 1.0
K A:K203 3.9 35.2 0.3
CB A:ASP192 3.9 30.8 1.0
HB2 A:ASP192 4.0 36.9 1.0
CA A:ASP192 4.0 26.9 1.0
OP2 b:G6 4.1 28.0 1.0
OD2 A:ASP192 4.1 34.0 1.0
O A:VAL72 4.1 21.5 1.0
N A:TYR193 4.2 28.1 1.0
O5' b:C5 4.2 22.9 1.0
HG22 A:ILE189 4.2 29.5 1.0
O A:HOH313 4.3 23.6 0.3
CB A:ASP71 4.4 21.5 1.0
O3' B:U4 4.4 24.6 1.0
OD2 A:ASP132 4.4 25.1 1.0
O A:HOH432 4.5 35.6 1.0
HB3 A:ASP71 4.5 25.8 1.0
C5' b:C5 4.6 22.9 1.0
HB2 A:TYR193 4.6 44.4 1.0
HD1 A:TYR193 4.6 32.0 1.0
HB2 A:ASP71 4.7 25.8 1.0
C A:ASP192 4.7 30.0 1.0
HG21 A:ILE189 4.7 29.5 1.0
CG2 A:ILE189 4.8 24.6 1.0
HB3 A:ASP192 4.9 36.9 1.0
H A:VAL72 4.9 24.7 1.0
HG23 A:ILE189 5.0 29.5 1.0

Magnesium binding site 2 out of 2 in 6dpn

Go back to Magnesium Binding Sites List in 6dpn
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg202

b:23.8
occ:1.00
OD1 A:ASP71 2.1 25.6 1.0
OD1 A:ASP132 2.3 22.2 1.0
OE1 A:GLU109 2.3 24.7 1.0
OP1 b:C5 2.6 25.7 1.0
O3' B:U4 2.6 24.6 1.0
CG A:ASP132 3.0 19.9 1.0
OD2 A:ASP132 3.0 25.1 1.0
CD A:GLU109 3.1 25.4 1.0
CG A:ASP71 3.1 24.8 1.0
P b:C5 3.2 26.2 1.0
OE2 A:GLU109 3.2 22.9 1.0
O A:HOH346 3.2 21.8 1.0
OD2 A:ASP71 3.6 24.1 1.0
HG12 A:VAL72 3.6 25.4 1.0
H A:VAL72 3.7 24.7 1.0
O A:HOH340 3.7 26.2 1.0
MG A:MG201 3.8 25.1 1.0
C3' B:U4 3.9 25.7 1.0
HA A:ASP71 4.0 22.9 1.0
O2' B:U4 4.0 22.0 1.0
C5' b:C5 4.1 22.9 1.0
O5' b:C5 4.2 22.9 1.0
C4' B:U4 4.2 25.0 1.0
H A:ASP132 4.3 20.4 1.0
HB2 A:SER133 4.3 26.4 1.0
CB A:ASP71 4.4 21.5 1.0
HG21 A:ILE189 4.4 29.5 1.0
OP2 b:C5 4.5 28.6 1.0
CB A:ASP132 4.5 18.8 1.0
CG1 A:VAL72 4.5 21.1 1.0
N A:VAL72 4.5 20.6 1.0
C2' B:U4 4.6 18.8 1.0
CG A:GLU109 4.6 19.6 1.0
HB2 A:ASP71 4.6 25.8 1.0
CA A:ASP71 4.6 19.1 1.0
O A:HOH339 4.7 26.1 1.0
HG13 A:VAL72 4.8 25.4 1.0
HG3 A:GLU109 4.8 23.5 1.0
C5' B:U4 4.8 24.9 1.0
HG2 A:GLU109 4.9 23.5 1.0
HG11 A:VAL72 4.9 25.4 1.0
HB3 A:ASP132 4.9 22.6 1.0
O A:VAL72 5.0 21.5 1.0
HG22 A:ILE189 5.0 29.5 1.0
N A:ASP132 5.0 17.0 1.0
HB2 A:ASP132 5.0 22.6 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Sep 30 23:25:15 2024

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