Magnesium in PDB 7xjg: Cryo-Em Structure of E.Coli Retron-EC86 in Complex with Its Effector at 2.5 Angstrom

All present enzymatic activity of Cryo-Em Structure of E.Coli Retron-EC86 in Complex with Its Effector at 2.5 Angstrom:
2.7.7.49;

The binding sites of Magnesium atom in the Cryo-Em Structure of E.Coli Retron-EC86 in Complex with Its Effector at 2.5 Angstrom (pdb code 7xjg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cryo-Em Structure of E.Coli Retron-EC86 in Complex with Its Effector at 2.5 Angstrom, PDB code: 7xjg:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cryo-Em Structure of E.Coli Retron-EC86 in Complex with Its Effector at 2.5 Angstrom


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of E.Coli Retron-EC86 in Complex with Its Effector at 2.5 Angstrom within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:35.3 occ:1.00 OD2 A:ASP198 2.6 34.7 1.0 OP1 D:DG85 2.8 37.5 1.0 CG A:ASP198 3.5 34.7 1.0 OD1 A:ASP198 3.8 34.7 1.0 OD1 A:ASN117 4.0 35.2 1.0 P D:DG85 4.1 37.5 1.0 OD1 A:ASP119 4.3 39.8 1.0 OD2 A:ASP119 4.3 39.8 1.0 O5' D:DG85 4.3 37.5 1.0 C5' D:DG85 4.4 37.5 1.0 CG A:ASP119 4.7 39.8 1.0 CB A:ASP198 4.8 34.7 1.0 OP2 D:DG85 4.9 37.5 1.0

Magnesium binding site 2 out of 2 in the Cryo-Em Structure of E.Coli Retron-EC86 in Complex with Its Effector at 2.5 Angstrom


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of E.Coli Retron-EC86 in Complex with Its Effector at 2.5 Angstrom within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg401 b:40.3 occ:1.00 OD2 B:ASP198 2.6 35.0 1.0 OP1 G:DG85 2.7 43.5 1.0 CG B:ASP198 3.4 35.0 1.0 OD1 B:ASP198 3.5 35.0 1.0 P G:DG85 4.1 43.5 1.0 OD1 B:ASN117 4.4 32.5 1.0 O5' G:DG85 4.5 43.5 1.0 C5' G:DG85 4.6 43.5 1.0 ND2 B:ASN117 4.6 32.5 1.0 OD1 B:ASP119 4.7 38.0 1.0 CB B:ASP198 4.8 35.0 1.0 CG B:ASN117 4.9 32.5 1.0 OP2 G:DG85 5.0 43.5 1.0

Y.Wang, Z.Guan, C.Wang, Y.Nie, Y.Chen, Z.Qian, Y.Cui, H.Xu, Q.Wang, F.Zhao, D.Zhang, P.Tao, M.Sun, P.Yin, S.Jin, S.Wu, T.Zou. Cryo-Em Structures of Escherichia Coli EC86 Retron Complexes Reveal Architecture and Defence Mechanism. Nat Microbiol V. 7 1480 2022.
ISSN: ESSN 2058-5276
PubMed: 35982312
DOI: 10.1038/S41564-022-01197-7