Magnesium in PDB 1i6h: Rna Polymerase II Elongation Complex

All present enzymatic activity of Rna Polymerase II Elongation Complex:
2.7.7.6;

The structure of Rna Polymerase II Elongation Complex, PDB code: 1i6h was solved by A.L.Gnatt, P.Cramer, R.D.Kornberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 3.30
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	157.300, 220.700, 191.300, 90.00, 97.50, 90.00
R / Rfree (%)	25 / 29.8

The structure of Rna Polymerase II Elongation Complex also contains other interesting chemical elements:

Zinc

(Zn)

8 atoms

The binding sites of Magnesium atom in the Rna Polymerase II Elongation Complex (pdb code 1i6h). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Rna Polymerase II Elongation Complex, PDB code: 1i6h:

Magnesium binding site 1 out of 1 in the Rna Polymerase II Elongation Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Rna Polymerase II Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ R:Mg10 b:47.1 occ:1.00 O3' R:C8 2.1 90.0 1.0 OD2 A:ASP483 2.7 38.7 1.0 OD2 A:ASP481 2.7 50.4 1.0 OP1 R:A9 2.7 86.1 1.0 OD1 A:ASP485 2.8 37.5 1.0 P R:A9 2.9 84.0 1.0 C5' R:A9 2.9 92.1 1.0 OD1 A:ASP483 3.0 41.4 1.0 O2' R:C8 3.0 88.3 1.0 O5' R:A9 3.1 92.2 1.0 CG A:ASP483 3.2 36.5 1.0 OD1 A:ASP481 3.3 44.5 1.0 C3' R:C8 3.3 89.9 1.0 CG A:ASP481 3.3 41.1 1.0 OD2 A:ASP485 3.5 38.8 1.0 CG A:ASP485 3.6 34.6 1.0 C4' R:C8 3.7 85.9 1.0 C2' R:C8 3.7 86.4 1.0 C4' R:A9 4.3 90.0 1.0 OP2 R:A9 4.4 88.6 1.0 C5' R:C8 4.5 86.5 1.0 CB A:ASP483 4.6 27.4 1.0 O4' R:A9 4.7 90.0 1.0 CB A:ASP481 4.7 29.7 1.0 O4' R:C8 4.8 87.3 1.0 C1' R:C8 4.8 88.3 1.0 O A:ASP481 4.9 25.6 1.0

A.L.Gnatt, P.Cramer, J.Fu, D.A.Bushnell, R.D.Kornberg. Structural Basis of Transcription: An Rna Polymerase II Elongation Complex at 3.3 A Resolution. Science V. 292 1876 2001.
ISSN: ISSN 0036-8075
PubMed: 11313499
DOI: 10.1126/SCIENCE.1059495