Magnesium in PDB 1s77: T7 Rnap Product Pyrophosphate Elongation Complex

All present enzymatic activity of T7 Rnap Product Pyrophosphate Elongation Complex:
2.7.7.6;

The structure of T7 Rnap Product Pyrophosphate Elongation Complex, PDB code: 1s77 was solved by Y.W.Yin, T.A.Steitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.69
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	127.738, 141.843, 142.856, 90.00, 90.00, 90.00
R / Rfree (%)	25.1 / 28.5

The binding sites of Magnesium atom in the T7 Rnap Product Pyrophosphate Elongation Complex (pdb code 1s77). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the T7 Rnap Product Pyrophosphate Elongation Complex, PDB code: 1s77:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the T7 Rnap Product Pyrophosphate Elongation Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of T7 Rnap Product Pyrophosphate Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ R:Mg902 b:39.7 occ:1.00 O2P R:3DA1 1.9 83.2 1.0 C3' R:A2 2.2 81.8 1.0 P R:3DA1 2.4 80.0 1.0 C5' R:A2 2.4 90.0 1.0 O3' R:A2 2.4 80.5 1.0 C4' R:A2 2.7 84.3 1.0 O1P R:3DA1 2.9 81.9 1.0 O5' R:A2 3.2 98.8 1.0 C2' R:A2 3.7 79.3 1.0 O4' R:A2 3.8 81.0 1.0 O5' R:3DA1 3.9 77.5 1.0 C1' R:A2 4.2 75.9 1.0 N9 R:A2 4.5 69.9 1.0 O2' R:A2 4.6 80.8 1.0 C8 R:A2 4.6 67.6 1.0 C5' R:3DA1 4.6 70.6 1.0 P R:A2 4.7 95.1 1.0 C8 R:3DA1 4.8 43.4 1.0

Magnesium binding site 2 out of 2 in the T7 Rnap Product Pyrophosphate Elongation Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of T7 Rnap Product Pyrophosphate Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg903 b:62.0 occ:1.00 OD2 D:ASP812 2.2 56.1 1.0 OD2 D:ASP537 2.5 60.1 1.0 O D:HOH904 2.7 54.5 1.0 O1P R:3DA1 2.8 81.9 1.0 CG D:ASP812 3.1 53.5 1.0 O D:GLY538 3.2 44.7 1.0 CG D:ASP537 3.3 57.5 1.0 OD1 D:ASP537 3.3 57.8 1.0 OD1 D:ASP812 3.4 57.7 1.0 O4 D:POP901 3.4 0.2 1.0 C5' R:3DA1 3.5 70.6 1.0 O5' R:3DA1 3.6 77.5 1.0 P R:3DA1 3.6 80.0 1.0 O5 D:POP901 3.7 0.4 1.0 P2 D:POP901 4.1 0.6 1.0 O2P R:3DA1 4.2 83.2 1.0 C D:GLY538 4.4 47.3 1.0 CB D:ASP812 4.5 49.3 1.0 N D:CYS540 4.7 49.8 1.0 CB D:ASP537 4.7 51.5 1.0 O D:POP901 4.8 0.3 1.0 O3 D:POP901 4.8 0.3 1.0 N D:GLY538 4.9 47.5 1.0 C4' R:3DA1 4.9 65.4 1.0

Y.W.Yin, T.A.Steitz. The Structural Mechanism of Translocation and Helicase Activity in T7 Rna Polymerase. Cell(Cambridge,Mass.) V. 116 393 2004.
ISSN: ISSN 0092-8674
PubMed: 15016374
DOI: 10.1016/S0092-8674(04)00120-5